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Staining of Urinary Sediment Constituents

  • Josefine NeuendorfEmail author
Chapter
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Abstract

In the absence of a phase-contrast microscope, urine sediment can alternatively be stained using specific staining techniques. This also permits better identification of morphological differences.

Keywords

Staining techniques Sternheimer-Malbin solution Fat staining Papanicolaou stain Urine cytology and erythrocyte morphology 

8.1 Staining Techniques

8.1.1 From the KOVA® System: Staining Solution (Sternheimer-Malbin Solution) (Fig. 8.1)

  • Staining solution: Ethanol, crystal violet, safranin O, and ammonium oxalate.

  • Shelf-life: Room temperature, no filtration needed.

  • Performance: Between 1 and 2 drops of staining solution are added to 1 ml of urine sediment and carefully mixed; microscopy analysis can be performed immediately.

  • Staining result: See product information leaflet.

Fig. 8.1

KOVA® stain

8.1.2 Fat Staining (Fig. 8.2)

  • Staining solution: Sudan IV.

  • Shelf-life: Room temperature.

  • Performance: Between 1 and 2 drops of filtered staining solution are added to 1 ml of urine sediment and carefully mixed; microscopy analysis can be performed immediately.

  • Staining result: Fat stains yellowish-red.

Fig. 8.2

Fat staining with Sudan IV

8.1.3 Papanicolaou Stain (Complex Stain) (Fig. 8.3)

  • For urine cytology and erythrocyte morphology.

Fig. 8.3

Papanicolaou staining

Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.Neuendorf Labordiagnostik WieslochGermany

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