Abstract
Adult mesenchymal stem cells for regenerative purposes can be isolated from different human tissues. However, those derived from adipose tissue (ASC) are currently more attractive from the clinical point of view, due to their low immunogenicity and the ease obtaining by means of minimally invasive surgical techniques together with a simple process of cellular isolation. In order to obtain a purified primary culture of ASC from the isolated stromal vascular fraction (SVF) mixture, in vitro cell cluture protocols have been developed to select and amplify the adherent cells using specific conditions. This chapter includes a description of cell expansion procedures and how donor characteristics influence the process and final ASC yield. Besides, the great therapeutic advantages of ASC compared to SVF are also exposed, as the low immunogenic character of these ASCs confers them the capacity to be used in allogeneic cell therapy.
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Diana, MR., Itxaso, G., BegoƱa, C. (2019). Regenerative Medicine Procedures for Aesthetic Physicians. In: Pinto, H., Fontdevila, J. (eds) Regenerative Medicine Procedures for Aesthetic Physicians. Springer, Cham. https://doi.org/10.1007/978-3-030-15458-5_19
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