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Glimpse on Genomics and Breeding in Bitter Gourd: A Crop of the Future for Food, Nutrition and Health Security

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Part of the Compendium of Plant Genomes book series (CPG)

Abstract

Bitter gourd, Momordica charantia L., family Cucurbitaceae, plausibly originated in eastern Asia, is traditionally cultivated as a vegetable and medicinal crop in tropical and subtropical areas in Asia, South America, East Africa, and the Caribbean. It has a simple genome with 2n = 22 chromosomes having a genome size of around 339 Mb. All parts of this plant, mainly the fruits and the seeds, contain more than 60 phytomedicines active against more than 30 diseases including cancer and diabetes. Single plant selection, mass selection, pedigree selection, and bulk population improvement are common methods are used widely in the bitter gourd improvement program. Recent discovery of gynoecious lines and their genetics will facilitate hybrid breeding. Ample genetic diversity has been found to exist in this crop as assessed by the use of molecular markers. Association mapping led to the detection of molecular markers linked to some fruit traits and content of a couple of phytomedicines. A few molecular genetic maps have been constructed and a number of agroeconomically important qualitative and quantitative fruit traits have been mapped. Recently, a draft genome sequence has also been reported and a few studies on genotyping by sequencing and RAD sequencing have been accomplished.

Keywords

Momordica charantia Phytomedicine Genetic diversity Molecular maps Gene mapping QTL Draft sequence GBS RAD-seq 

References

  1. Basch E, Garbardi S, Ulbricht C (2003) Bitter melon (Momordica charantia): a review of efficacy and safety. Amer J Health-Syst Pharm 60:356–359CrossRefGoogle Scholar
  2. Behera TK, Behera S, Bharathi LK, Joseph JK (2010) Bitter gourd: botany, horticulture and breeding. In: Janick J (ed) Horticulture reviews. Wiley, Blackwell, pp 101–141Google Scholar
  3. Behera TK, Dey SS, Munshi AD, Gaikwad AB, Pal A, Singh I (2009) Sex inheritance and development of gynoecious hybrids in bitter gourd (Momordica charantia L.). Sci Hort 120:130–133CrossRefGoogle Scholar
  4. Behera TK, Dey SS, Sirohi PS (2006) DBGy-201 and DBGy-202: two gynoecious lines in bitter gourd (Momordica charantia L.) isolated from indigenous source. Indian J Genet 66:61–62Google Scholar
  5. Chakravarty HL (1990) Cucurbits of India and their role in the development of vegetable crops. In: Bates DM, Robinson RW, Jeffrey C (eds) Biology and utilization of cucurbitaceae. Cornell University Press, Ithaca, NY, pp 325–334Google Scholar
  6. Cui J, Luo S, Niu Y, Huang R, Wen Q, Su J et al (2018) A RAD-based genetic map for anchoring scaffold sequences and identifying QTLs in bitter gourd (Momordica charantia). Front Plant Sci 9:477.  https://doi.org/10.3389/fpls.2018.00477CrossRefPubMedPubMedCentralGoogle Scholar
  7. Decker-Walters DS (1999) Cucurbits, sanskrit, and the Indo-Aryas. Econ Bot 53:98–112CrossRefGoogle Scholar
  8. Dey SS, Singh AK, Chandel D, Behera TK (2006) Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits. Sci Hort 109:21–28CrossRefGoogle Scholar
  9. Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES et al (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6:e19379.  https://doi.org/10.1371/journal.pone.0019379CrossRefPubMedPubMedCentralGoogle Scholar
  10. Gaikwad AB, Behera TK, Singh AK, Chandel D, Karihaloo JL, Staub JE (2008) AFLP analysis provides strategies for improvement of bitter gourd (Momordica charantia L.). HortScience 43:127–133CrossRefGoogle Scholar
  11. Gangadhara Rao P, Behera TK, Gaikwad AB, Munshi AD, Jat GS, Boopalakrishnan G (2018) Mapping and QTL analysis of gynoecy and earliness in bitter gourd (Momordica charantia L.) using genotyping-by-sequencing (GBS) technology. Front Plant Sci 9:1555.  https://doi.org/10.3389/fpls.2018.01555CrossRefPubMedPubMedCentralGoogle Scholar
  12. Grover JK, Yadav SP (2004) Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol 93:123–132CrossRefGoogle Scholar
  13. Grover JK, Rathi SS, Vats V (2002) Amelioration of experimental diabetic neuropathy and gastropathy in rats following oral administration of plant (Eugenia jambolana, Mucuna pruriens and Tinospora cordifolia) extracts. Indian J Exp Biol 40:273–276PubMedGoogle Scholar
  14. He J, Zhao X, Laroche A, Lu ZX, Liu H, Li Z (2014) Geno typing by—sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding. Front Plant Sci 5:484.  https://doi.org/10.3389/fpls.2014.00484CrossRefPubMedPubMedCentralGoogle Scholar
  15. Heiser CB (1979) The gourd book. University of Oklahoma Press, Norman, OKGoogle Scholar
  16. Iwamoto E, Ishida T (2005) Bisexual flower induction by the application of silver nitrate in gynoecious balsam pear (Momordica charantia L.). Hort Res (Japan) 4:391–395CrossRefGoogle Scholar
  17. Jha UC, Roy RP (1989) Hermaphrodite flowers in dioecious Momordica dioica Roxb. Curr Sci 58:1249–1250Google Scholar
  18. Kajale MD (1991) Current status of Indian palaeoethnobotany: introduced and indigenous food plants with a discussion of the historical and evolutionary development of India agriculture and agricultural systems in general. In: Renfrew JM (ed) New light on early farming. Edinburgh University Press, Edinburgh, UK, Recent Developments in Palaeoethnobotany, pp 155–189Google Scholar
  19. Kole C, Bode AO, Kole P, Rao VK, Bajpai A, Backiyarani S (2012) The first genetic map and positions of major fruit trait loci of bitter melon (Momordica charantia). J Plant Sci Mol Breed 1:1–6.  https://doi.org/10.7243/2050-2389-1-1CrossRefGoogle Scholar
  20. Kole C, Kole P, Randunu KM, Choudhary P, Podila R, Ke PC, Rao AM, Marcus RK (2013) Nanobiotechnology can boost crop production and quality: first evidence from increased plant biomass, fruit yield and phytomedicine content in bitter melon (Momordica charantia). BMC Biotechnol 26(13):37.  https://doi.org/10.1186/1472-6750-13-37CrossRefGoogle Scholar
  21. Marr KL, Xia YM, Bhattarai NK (2004) Allozyme, morphological and nutritional analysis bearing on the domestication of Momordica charantia L. (Cucurbitaceae). Econ Bot 58:435–455CrossRefGoogle Scholar
  22. Matsumura H, Miyagi N, Taniai N, Fukushima M, Tarora K, Shudo A (2014) Mapping of the gynoecy in bitter gourd (Momordica charantia) using RAD-Seq analysis. PLoS ONE 9:e87138.  https://doi.org/10.1371/journal.pone.0087138CrossRefPubMedPubMedCentralGoogle Scholar
  23. Miniraj N, Prasanna KP, Peter KV (1993) Bitter gourd (Momordica spp.). In: Kalloo G, Bergh BO (eds) Genetic improvement of vegetable crops. Pergamon Press, Oxford, pp 239–246Google Scholar
  24. Ram D, Kumar S, Banerjee MK, Kalloo G (2002) Occurrence, identification and preliminary characterization of gynoecism in bitter gourd (Momordica charantia L.). Indian J Agri Sci 72:348–349Google Scholar
  25. Raman A, Lau C (1996) Anti-diabetic properties and phytochemistry of Momordica charantia L. (Cucurbitaceae). Phytomedicine 2:349–362CrossRefGoogle Scholar
  26. Reyes MEC, Gildemacher BH, Jansen GJ (1994) Momordica L. In: Siemonsma JS, Piluek K (eds) Plant resources of South-East Asia: vegetables. Pudoc Scientific Publishers, Wageningen, the Netherlands, pp 206–210Google Scholar
  27. Roy SK (1973) A simple and rapid method for estimation of total carotenoids pigments in mango. J Food Sci Technol 10:45Google Scholar
  28. Sathishsekar D, Subramanian S (2005) Antioxidant properties of Momordica Charantia (bitter gourd) seeds on streptozotocin induced diabetic rats. Asia Pac J Clin Nutr 14(2):153–158PubMedGoogle Scholar
  29. Semiz A, Sen A (2007) Antioxidant and chemoprotective properties of Momordica charantia L. (bitter melon) fruit extract. Afr J Biotechnol 6(3):273–277Google Scholar
  30. Singh AK, Behera TK, Chandel D, Sharma P, Singh NK (2007) Assessing genetic relationships among bitter gourd (Momordica charantia L.) accessions using inter simple sequence repeat (ISSR) markers. J Hort Sci Biotechnol 82:217–222CrossRefGoogle Scholar
  31. Sirohi PS (1997) Improvement in cucurbit vegetables. Indian Hort 42:64–67Google Scholar
  32. Tindall HD (1983) Vegetables in the tropics. Macmillan, LondonCrossRefGoogle Scholar
  33. Urasaki N, Takagi H, Natsume S, Uemura A, Taniai N, Miyagi N et al (2017) Draft genome sequence of bitter gourd (Momordica charantia), a vegetable and medicinal plant in tropical and subtropical regions. DNA Res 24(1):51–58.  https://doi.org/10.1093/dnares/dsw047CrossRefGoogle Scholar
  34. Walters TW, Decker-Walters DS (1988) Balsam-pear (Momordica charantia, Cucurbita- ceae). Econ Bot 42(2):286–292CrossRefGoogle Scholar
  35. Wang QM, Zeng GW (1997) Hormonal regulation of sex differentiation on Momordica charantia L. J Zhejiang Agri Univ 23:551–556Google Scholar
  36. Wang Z, Xiang C (2013) Genetic mapping of QTLs for horticulture traits in a F2-3 population of bitter gourd (Momordica charantia L.). Euphytica 193:235–250.  https://doi.org/10.1007/s10681-013-0932-0CrossRefGoogle Scholar
  37. Yang SL, Walters TW (1992) Ethnobotany and the economic role of the Cucurbitaceae of China. Econ Bot 46:349–367CrossRefGoogle Scholar
  38. Yonemori S, Fujieda K (1985) Sex expression in Momordica charantia L. Sci Bull Coll Agri, Univ Ryukyus, Okinawa 32:183–187Google Scholar

Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.Division of Vegetable ScienceICAR-Indian Agricultural Research InstitutePusaIndia
  2. 2.Gene Research Center, Shinshu UniversityUeda, NaganoJapan
  3. 3.ICAR-National Institute for Plant BiotechnologyPusaIndia

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