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Gene-Editing Techniques

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Success in Academic Surgery: Basic Science

Part of the book series: Success in Academic Surgery ((SIAS))

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Abstract

This chapter will broadly cover the newest genome editing technology, clustered regularly interspaced short palindromic repeats (CRISPR), and CRISPR-associated system (Cas), as a critical tool for a modern research laboratory. Multiplex gene mutagenesis, tissue-specific gene disruption, DNA insertions, transcriptional activation, transcriptional repression, megabase-sized deletions, translocations, and genetic screens are all possible with CRISPR/Cas technology. How CRISPR-genome editing works, its applications, and considerations for best use will be discussed.

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Author information

Authors and Affiliations

  1. Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN, USA

    Kevin W. Freeman

Authors
  1. Kevin W. Freeman
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Corresponding author

Correspondence to Kevin W. Freeman .

Editor information

Editors and Affiliations

  1. Division of Gastrointestinal Surgery, University of Alabama, Birmingham, AL, USA

    Gregory Kennedy

  2. Le Bonheur Children’s Hospital, University of Tennessee Health Science Centre, Memphis, TN, USA

    Ankush Gosain

  3. UNC School of Medicine, University of North Carolina, Chapel Hill, NC, USA

    Melina Kibbe

  4. Baylor College of Medicine, Houston, TX, USA

    Scott A. LeMaire

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Freeman, K.W. (2019). Gene-Editing Techniques. In: Kennedy, G., Gosain, A., Kibbe, M., LeMaire, S. (eds) Success in Academic Surgery: Basic Science. Success in Academic Surgery. Springer, Cham. https://doi.org/10.1007/978-3-030-14644-3_11

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  • DOI: https://doi.org/10.1007/978-3-030-14644-3_11

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-14643-6

  • Online ISBN: 978-3-030-14644-3

  • eBook Packages: MedicineMedicine (R0)

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