Effective Cell Culture
Cell culture is a powerful tool for investigation. For the surgeon scientist, it may offer a shorter turnaround time in test results, a cheaper model than other in vivo methods (e.g., large animal models), and can offer the specificity that comes with isolating a cellular process. Suffice it to say that it is an invaluable skill to have when starting a lab. In this chapter we introduce why cell cultures make sense for the surgeon scientist, how to go about thinking about cell cultures, and tips and tricks to getting started with this technology. Many theoretical examples are used to solidify certain concepts, and procedural protocols are included to get you started. These are certainly not a comprehensive analysis of everything that is needed in the lab; however, it is a solid background for those wishing to begin their career as a surgeon scientist.
KeywordsCell culture Passage Subculture Cell line
We would like to take the opportunity to thank the previous authors, Edward S. Moreira, Ph.D., and Nick D. Tsihlis, Ph.D., for their contributions.
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