Effective Cell Culture

  • Patrick B. Schwartz
  • Sean M. Ronnekleiv-KellyEmail author
Part of the Success in Academic Surgery book series (SIAS)


Cell culture is a powerful tool for investigation. For the surgeon scientist, it may offer a shorter turnaround time in test results, a cheaper model than other in vivo methods (e.g., large animal models), and can offer the specificity that comes with isolating a cellular process. Suffice it to say that it is an invaluable skill to have when starting a lab. In this chapter we introduce why cell cultures make sense for the surgeon scientist, how to go about thinking about cell cultures, and tips and tricks to getting started with this technology. Many theoretical examples are used to solidify certain concepts, and procedural protocols are included to get you started. These are certainly not a comprehensive analysis of everything that is needed in the lab; however, it is a solid background for those wishing to begin their career as a surgeon scientist.


Cell culture Passage Subculture Cell line 



We would like to take the opportunity to thank the previous authors, Edward S. Moreira, Ph.D., and Nick D. Tsihlis, Ph.D., for their contributions.


  1. 1.
    Hotamisligil GS. The role of TNFalpha and TNF receptors in obesity and insulin resistance. J Intern Med. 1999;245(6):621–5.CrossRefGoogle Scholar
  2. 2.
    Knight DM, Trinh H, Le J, Siegel S, Shealy D, McDonough M, et al. Construction and initial characterization of a mouse-human chimeric anti-TNF antibody. Mol Immunol. 1993;30(16):1443–53.CrossRefGoogle Scholar
  3. 3.
    Bernard GR, Vincent JL, Laterre PF, LaRosa SP, Dhainaut JF, Lopez-Rodriguez A, et al. Efficacy and safety of recombinant human activated protein C for severe sepsis. N Engl J Med. 2001;344(10):699–709. Scholar
  4. 4.
    Davis JM, editor. Basic cell culture. New York, NY: Oxford Press; 2002.Google Scholar
  5. 5.
    Davis JM, editor. Animal cell culture: essential methods. Hoboken, NJ: John Wiley & Sons; 2011.Google Scholar
  6. 6.
    Helgason CD, Miller CL, editors. Basic cell culture protocols. New York, NY: Humana; 2005.Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Patrick B. Schwartz
    • 1
  • Sean M. Ronnekleiv-Kelly
    • 2
    Email author
  1. 1.Department of SurgeryUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA
  2. 2.Division of Surgical Oncology, Department of General SurgeryUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA

Personalised recommendations