Abstract
Rapid urbanization has resulted in an increase of municipal sewage discharge, which, in turn, has added load and cost to the conventional water treatment processes. The composition of municipal sewage mostly contains natural inorganic and organic minerals as well as synthetic compounds. Microalgae utilize these wastes as nutritional sources and hence could be used as an interesting step to improve the quality of sewage. Though there are some natural algal flora existing in sewage, few selective and efficient strains could be used in this purpose. They are non-pathogenic and have the potential to eliminate pathogens by competitive growth. Moreover, they could reduce biological and chemical oxygen demand of water as well as remove heavy metals by algal metabolism. Unlike conventional methods, it requires low operational and maintenance cost and no use of hazardous chemicals for water treatment. Additionally, the biomass could be utilized to generate value-added products such as bioenergy, pharmaceuticals, nutraceuticals, etc. However, land requirement, difficulties in the growth of pure strains, variation in environmental factors, eutrophication, self-shading and difficulties in the harvesting of biomass are some of the bottlenecks of this process. With recent advances in scientific knowledge, sophisticated techniques and environmental awareness, microalgae could offer a sustainable, environment-friendly solution to treat wastewater which could be further enhanced by the addition of other organisms and aquatic plants.
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References
Abdel-Raouf N, Al-Homaidan AA, Ibraheem IBM (2012) Microalgae and wastewater treatment. Saudi J Biol Sci 19(3):257–275
Afkar E, Ababna H, Fathi AA (2010) Toxicological response of the green alga Chlorella vulgaris, to some heavy metals. Am J Environ Sci 6(3):230–237
Anastopoulos I, Kyzas GZ (2015) Progress in batch biosorption of heavy metals onto algae. J Mol Liq 209:77–86
Anonymous (2008) MOEF, Government of India. National Urban Sanitation Policy
Ansari AA, Khoja AH, Nawar A et al (2017) Wastewater treatment by local microalgae strains for CO2 sequestration and biofuel production. Appl Water Sci 7(7):4151–4158
Arceivala SJ, Asolekar SR (2010) Wastewater treatment for pollution control and reuse. Tata McGraw Hill Education Pvt Ltd, New Delhi
Azov Y, Shelef G (1987) The effect of pH on the performance of high-rate oxidation ponds. Water Sci Technol 19(12):381–383
Barbosa RA, Sant’Anna GL Jr (1989) Treatment of raw domestic sewage in an UASB reactor. Water Res 23(12):1483–1490
Berger MR, Schmähl D, Edler L (1990) Implications of the carcinogenic hazard of low doses of three hepatocarcinogenic N- Nitrosamines. Cancer Sci 81(6–7):598–606
Bishnoi NR, Kumar R, Kumar S, Rani S (2007) Biosorption of Cr (III) from aqueous solution using algal biomass Spirogyra spp. J Hazard Mater 145(1–2):142–147
Bishop WM, Zubeck HM (2012) Evaluation of microalgae for use as nutraceuticals and nutritional supplements. J Nutr Food Sci 2(5):1–6
Borowitzka MA, Borowitzka LJ (1988) Micro-algal biotechnology. Cambridge University Press, Cambridge
Bosman J, Hendricks F (1980) The development of an algal pond system for the removal of nitrogen from an inorganic industrial; effluent. In: Proceedings of international symposium on aquaculture in wastewater NIWP. CSIR, Pretoria, pp 26–35
Chevalier P, de la Noue J (1985) Wastewater nutrient removal with microalgae immobilized in carrageenan. Enzym Microb Technol 7(12):621–624
Choksi KN, Sheth MA, Mehta D (2015) To evaluate the performance of sewage treatment plant: a case study of Surat city. Int J Eng Technol 2(8):1076–1080
Colak O, Kaya Z (1988) A study on the possibilities of biological wastewater treatment using algae. Doga Biyoloji Serisi 12:18–29
Danquah MK, Gladman B, Moheimani N, Forde GM (2009) Microalgal growth characteristics and subsequent influence on dewatering efficiency. Chem Eng J 151(1–3):73–78
Dasgupta CN (2015) Algae as a source of phycocyanin and other industrially important pigments. In: Algal Biorefinery: An Integrated Approach. Springer, Cham, pp 253–276
Dasgupta CN, Suseela MR, Mandotra SK et al (2015) Dual uses of microalgal biomass: an integrative approach for biohydrogen and biodiesel production. Appl Energy 146:202–208
de la Noue J, Basseres A (1989) Biotreatment of anaerobically digested swine manure with microalgae. Biol Wastes 29(1):17–31
de la Noue J, de Pauw N (1988) The potential of microalgal biotechnology: a review of production and uses of microalgae. Biotechnol Adv 6(4):725–770
de la Noue J, Proulx D (1988) Biological tertiary treatment of urban wastewaters with chitosan-immobilized Phormidium. Appl Microbiol Biotechnol 29(2–3):292–297
de la Noüe J, Laliberté G, Proulx D (1992) Algae and waste water. J Appl Phycol 4(3):247–254
de Pauw N, Van Vaerenbergh E (1983) Microalgal wastewater treatment systems: potentials and limits. In: Internation convention on Phytodepurization and the use of the produced biomass, Parma, pp 211–287
Demirbas MF (2011) Biofuels from algae for sustainable development. Appl Energy 88(10):3473–3480
Drizo AFCA, Frost CA, Smith KA et al (1997) Phosphate and ammonium removal by constructed wetlands with horizontal subsurface flow, using shale as a substrate. Water Sci Technol 35(5):95–102
El-Enany AE, Issa AA (2000) Cyanobacteria as a biosorbent of heavy metals in sewage water. Environ Toxicol Pharmacol 8(2):95–101
Ergashev AE, Tajiev SH (1986) Seasonal variations of phytoplankton in a series of waste treatment lagoons (Chimkent, Central Asia) Part 2: Distribution of phytoplankton numbers and biomass. CLEAN–Soil, Air, Water 14(6):613–625
Fierro S, del Pilar Sánchez-Saavedra M, Copalcua C (2008) Nitrate and phosphate removal by chitosan immobilized Scenedesmus. Bioresour Technol 99(5): 1274–1279
Fogg GE (1975) Primary productivity. Chem Oceanogr 2:385–453
Fytili D, Zabaniotou A (2008) Utilization of sewage sludge in EU application of old and new methods - a review. Renew Sustain Energy Rev 12(1):116–140
Garbisu C, Hall DO, Serra JL (1993) Removal of phosphate by foam immobilized Phormidium laminosum in batch and continuous flow bioreactors. J Chem Technol Biotechnol 57(2):181–189
Garbisu C, Hall DO, Llama MJ et al (1994) Inorganic nitrogen and phosphate removal from water by free-living and polyvinyl-immobilized Phormidium laminosum in batch and continuous-flow bioreactors. Enzym Microb Technol 16(5):395–401
Gehm HW (1945) Characteristics of Sewage. Sewage Work J 17(5):984–987
Gentili FG, Fick J (2017) Algal cultivation in urban wastewater: an efficient way to reduce pharmaceutical pollutants. J Appl Phycol 29(1):255–262
González LE, Cañizares RO, Baena S (1997) Efficiency of ammonia and phosphorus removal from a Colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus. Bioresour Technol 60(3):259–262
Gray BE (1989) A primer on California water transfer law. Ariz L Rev 31:745–781
Grobbelaar JU (2004) Algal nutrition - mineral nutrition. In: Richmond A (ed) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Publishing Ltd, Ames, pp 95–115
Habib MAB, Parvin M, Huntington TC et al (2008) A review on culture, production and use of Spirulina as food for humans and feeds for domestic animals and fish. Food and agriculture organization of the United Nations. J Clean Prod 91:1–11
Hallegraeff GM (1993) A review of harmful algal blooms and their apparent global increase. Phycologia 32(2):79–99
Hameed MSA (2002) Effect of immobilization on growth and photosynthesis of the green alga Chlorella vulgaris and its efficiency in heavy metals removal. Bull Fac Sci Assiut Univ 31(1-D):233–240
Hamner S, Tripathi A, Mishra RK et al (2006) The role of water use patterns and sewage pollution in incidence of water-borne enteric diseases along the Ganges River in Varanasi, India. Int J Environ Health Res 16(2):113–132
Hattab MA, Ghaly A, Hammoud A et al (2015) Microalgae harvesting methods for industrial production of biodiesel: critical review and comparative analysis. J Fundam Renew Energy Appl 5(154):10–4172
He J, Chen JP (2014) A comprehensive review on biosorption of heavy metals by algal biomass: materials, performances, chemistry, and modeling simulation tools. Bioresour Technol 160:67–78
Horan NJ (1989) Biological wastewater treatment systems: theory and operation. Wiley, New York
Hvitved-Jacobsen T (1982) The impact of combined sewer overflows on the dissolved oxygen concentration of a river. Water Res 16(7):1099–1105
Jensen A (1993) Present and future needs for algae and algal products. In: Fourteenth International Seaweed Symposium. Springer, Dordrecht, pp 15–23
Kaur R, Wani SP, Singh AK et al (2012) Wastewater production, treatment and use in India. In National Report presented at the 2nd regional workshop on Safe Use of Wastewater in Agriculture
Kiran B, Rani N, Kaushik A (2008) Chromium (VI) tolerance in two halotolerant strains of Nostoc. J Environ Biol 29(2):155–158
Kshirsagar AD (2013) Bioremediation of wastewater by using microalgae: an experimental study. Int J Life Sci Biotechnol Pharm 2(3):339–346
Kumar D, Gaur JP (2011) Chemical reaction and particle diffusion based kinetic modeling of metal biosorption by a Phormidium sp. dominated cyanobacterial mat. Bioresour Technol 102(2):633–640
Lau PS, Tam NFY, Wong YS (1996) Wastewater nutrients removal by Chlorella vulgaris: optimization through acclimation. Environ Technol 17(2):183–189
Lazarevic D, Aoustin E, Buclet N et al (2010) Plastic waste management in the context of a European recycling society: comparing results and uncertainties in a life cycle perspective. Resour Conserv Recycl 55(2):246–259
Li Y, Chen YF, Chen P et al (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour Technol 102(8):5138–5144
Lima SA, Raposo MFJ, Castro PM et al (2004) Biodegradation of p-chlorophenol by a microalgae consortium. Water Res 38(1):97–102
Mahapatra DM, Chanakya HN, Ramachandra TV (2014) Bioremediation and lipid synthesis through mixotrophic algal consortia in municipal wastewater. Bioresour Technol 168:142–150
Malina JF, Yousef YA (1964) The fate of coliform organisms in waste stabilization ponds. J Water Pollut Control Fed 36:1432–1442
Mallick N, Rai LC (1993) Influence of culture density, pH, organic acids and divalent cations on the removal of nutrients and metals by immobilized Anabaena doliolum and Chlorella vulgaris. World J Microbiol Biotechnol 9(2):196–201
Mane PC, Bhosle AB (2012) Bioremoval of some metals by living algae Spirogyra sp. and Spirullina sp. from aqueous solution. Int J Environ Res 6(2):571–576
Mehta SK, Gaur JP (2005) Use of algae for removing heavy metal ions from wastewater: progress and prospects. Crit Rev Biotechnol 25(3):113–152
Meron A, Rebhun M, Sless B (1965) Quality changes as a function of detention time in wastewater stabilization ponds. J Water Pollut Control 37(12):1657–1670
Miranda J, Krishnakumar G, Gonsalves R (2012) Cr 6+ bioremediation efficiency of Oscillatoria laetevirens (Crouan & Crouan) Gomont and Oscillatoria trichoides Szafer: kinetics and equilibrium study. J Appl Phycol 24(6):1439–1454
Moawad SK (1968) Inhibition of coliform bacteria by algal population in microoxidation ponds. Environ Health 10(2):106–112
Mohn FH (1980) Experiences and strategies in the recovery of biomass from mass cultures of microalgae biomass; production and use. In: Shelef G, Soeder CJ (eds) National Council for Research and Development, Israel and the Gesellschaft fur Strahlen-und Umweltforschung (GSF), Munich, Germany
Mohn FH (1988) Harvesting of micro-algal biomass. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge U.P., Cambridge, UK, pp 395–414
Monteiro CM, Castro PM, Malcata FX (2009) Use of the microalga Scenedesmus obliquus to remove cadmium cations from aqueous solutions. World J Microbiol Biotechnol 25(9):1573–1578
Monteiro CM, Fonseca SC, Castro PM et al (2011) Toxicity of cadmium and zinc on two microalgae, Scenedesmus obliquus and Desmodesmus pleiomorphus, from Northern Portugal. J Appl Phycol 23(1):97–103
Moss B (1973) The influence of environmental factors on the distribution of freshwater algae: an experimental study: II. The role of pH and the carbon dioxide-bicarbonate system. J Ecol 61(1):157–177
Munir N, Imtiaz A, Sharif N, Naz S (2015) Optimization of growth conditions of different algal strains and determination of their lipid contents. J Anim Plant Sci 25(2):546–553
Orhon D, Artan N, Ateş E (1994) A description of three methods for the determination of the initial inert particulate chemical oxygen demand of wastewater. J Chem Technol Biotechnol 61(1):73–80
Oswald WJ, Gotaas HB (1957) Photosynthesis in sewage treatment. Trans Am Soc Civ Eng 122(1):73–105
Palmer CM (1969) A composite rating of algae tolerating organic pollution. J Phycol 5(1):78–82
Palmer CM (1974) Algae in American sewage stabilization’s ponds. Rev Microbiol 5:75–80
Parhad NM, Rao NU (1976) Decrease of bacterial content in different types of stabilization ponds. Indian J Environ Health 18(1):33–46
Patil PN, Sawant DV, Deshmukh RN (2012) Physico-chemical parameters for testing of water – A review. Int J Environ Sci 3(3):1194–1207
Phang SM (1990) Algal production from agro-industrial and agricultural wastes in Malaysia. Ambio 19:415–418
Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102(1):17–25
Przytocka-Jusiak M, Duszota M, Matusiak K et al (1984) Intensive culture of Chlorella vulgaris as the second stage of biological purification of nitrogen industry wastewaters. Water Res 18(1):1–7
Ras M, Lardon L, Bruno S et al (2011) Experimental study on a coupled process of production and anaerobic digestion of Chlorella vulgaris. Bioresour Technol 102:200–206
Renaud SM, Thinh LV, Lambrinidis G et al (2002) Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures. Aquaculture 211(1–4):195–214
Rhoades JD (1996) Salinity: electrical conductivity and total dissolved solids. In: Methods of soil analysis part 3, chemical methods. Soil Science Society of America and American Society of Agronomy, USA, pp 417–435
Rogers HR (1996) Sources, behavior and fate of organic contaminants during sewage treatment and in sewage sledges. Sci Total Environ 185(1–3):3–26
Romera E, González F, Ballester A et al (2007) Comparative study of biosorption of heavy metals using different types of algae. Bioresour Technol 98(17):3344–3353
Rudolfs W, Falk L, Ragotzkie RA (1950) Literature review on the occurrence and survival of enteric, pathogenic, and relative organisms in soil, water, sewage, and sludges, and on vegetation: I. Bacterial and virus diseases. Sewage and Industrial Wastes, Water Environment Federation, New Jersey, pp 1261–1281
Sawayama S, Rao KK, Hall DO (1998) Nitrate and phosphate ion removal from water by Phormidium laminosum immobilized on hollow fibres in a photobioreactor. Appl Microbiol Biotechnol 49(4):463–468
Schwartz RE, Hirsch CF, Sesin DF et al (1990) Pharmaceuticals from cultured algae. J Ind Microbiol 5(2–3):113–123
Sebastian S, Nair KVK (1984) Total removal of coliforms and E. coli from domestic sewage by high-rate pond mass culture of Scenedesmus obliquus. Environ Pollut A - Ecol Biol 34(3):197–206
Sengar RMS, Singh KK, Singh S (2011) Application of phycoremediation technology in the treatment of sewage water to reduce pollution load. Ind J Sci Res 2(4):33–39
Sheehan J, Dunahay T, Benemann J et al (1998) Look back at the US department of energy’s aquatic species program: biodiesel from algae; close-out report (No. NREL/TP-580-24190). National Renewable Energy Lab., Golden, CO. (US)
Shelef G, Moraine R, Meydan A, Sandbank E (1977) Combined algae production-wastewater treatment and reclamation systems. In: Microbial energy conversion. American Society for Microbiology, USA, pp 427–442
Shi J, Podola B, Melkonian M (2007) Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study. J Appl Phycol 19(5):417–423
Singh SK, Bansal A, Jha MK et al (2012) An integrated approach to remove Cr (VI) using immobilized Chlorella minutissima grown in nutrient rich sewage wastewater. Bioresour Technol 104:257–265
Singh S, Ghosh NC, Krishan G et al (2015) Development of an overall water quality index (OWQI) for surface water in Indian context. Curr World Environ 10(3):813–822
Sivasubramanian V (2006) Phycoremediation of industrial effluents. http://phycoremediation.in/projects.html
Sivasubramanian V, Subramanian VV, Muthukumaran M et al (2012) Algal technology for effective reduction of total hardness in wastewater and industrial effluents. Phykos 42(1):51–58
Swetha C, Sirisha K, Swaminathan D, Sivasubramanian V (2016) Study on the treatment of dairy effluent using Chlorella vulgaris and production of biofuel (Algal treatment of dairy effluent). Biotechnol Ind J 21(1):12–17
Sydney ED, Da Silva TE, Tokarski A et al (2011) Screening of microalgae with potential for biodiesel production and nutrient removal from treated domestic sewage. Appl Energy 88(10):3291–3294
Talbot P, de la Noue J (1993) Tertiary treatment of wastewater with Phormidium bohneri (Schmidle) under various light and temperature conditions. Water Res 27(1):153–159
Tam NFY, Wong YS (1989) Wastewater nutrient removal by Chlorella pyrenoidosa and Scenedesmus sp. Environ Pollut 58(1):19–34
Tarlan E, Dilek FB, Yetis U (2002) Effectiveness of algae in the treatment of a wood-based pulp and paper industry wastewater. Bioresour Technol 84(1):1–5
Townsend SA, Boland KT, Wrigley TJ (1992) Factors contributing to a fish kill in the Australian wet/dry tropics. Water Res 26(8):1039–1044
Tubea B, Hawxby K, Mehta R (1981) The effects of nutrient, pH and herbicide levels on algal growth. Hydrobiol 79(3):221–227
Vignesh MS, Shivsankar R, Rao PR et al (2006) Phycoremediation of effluent from tannery and pharmaceutical industries – a lab study. Ind Hydrobiol 9(1):51–60
Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol 13(3):218–227
Wang B, Li Y, Wu N, Lan CQ (2008) CO2 bio-mitigation using microalgae. Appl Microbiol Biotechnol 79(5):707–718
Wang L, Min M, Li Y et al (2010) Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Appl Biochem Biotechnol 162(4):1174–1186
Wang HMD, Chen CC, Huynh P et al (2015) Exploring the potential of using algae in cosmetics. Bioresour Technol 184:355–362
Webber J (1972) Effects of toxic metals in sewage on crops. Water Pollut Control 71(4):404–413
Westholm LJ (2006) Substrates for phosphorus removal – potential benefits for on-site wastewater treatment. Water Res 40(1):23–36
Woertz I, Feffer A, Lundquist T et al (2009) Algae grown on dairy and municipal wastewater for simultaneous nutrient removal and lipid production for biofuel feedstock. J Environ Eng 135(11):1115–1122
Zhou GJ, Peng FQ, Zhang LJ et al (2012) Biosorption of zinc and copper from aqueous solutions by two freshwater green microalgae Chlorella pyrenoidosa and Scenedesmus obliquus. Environ Sci Pollut Res 19(7):2918–2929
Zhu Z, Liu J (2008) Remote monitoring system of urban sewage treatment based on Internet. In: Automation and logistics, 2008. ICAL 2008. IEEE International Conference on IEEE, pp 1151–1155
Acknowledgements
We thank the Director of CSIR-NBRI for providing laboratory facilities to Indo-US Science and Technology Forum, New Delhi, for the financial assistance under i-CRAFT project; to CSIR-Scientist’s Pool Scheme; to authorities of 345 MLD Bharwara Sewage Treatment Plant, Lucknow; and to Mr. Gurubachan and other members of Algology Laboratory for their cooperation during the study.
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Dasgupta, C.N., Toppo, K., Nayaka, S., Singh, A.K. (2019). Bioremediation of Municipal Sewage Using Potential Microalgae. In: Gupta, S.K., Bux, F. (eds) Application of Microalgae in Wastewater Treatment. Springer, Cham. https://doi.org/10.1007/978-3-030-13913-1_7
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