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Imaging Techniques for Probing Nanoparticles in Cells and Skin

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Biological Responses to Nanoscale Particles

Part of the book series: NanoScience and Technology ((NANO))

Abstract

Imaging techniques for probing the interactions of nanoparticles with cells and skin are essential for a qualitative and quantitative understanding of uptake and penetration processes. A variety of important visualization techniques is reviewed for providing an overview on established and recently developed techniques. This includes optical microscopy, fluorescence microscopy, electron microscopy, Raman microscopy, optical near-field microscopy, X-ray microscopy, as well as recent and emerging developments in the field of spectromicroscopy.

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Acknowledgements

We are grateful for the contributions of our collaborators contributing to some of the results shown. Specifically, we are indebted to Dr. F. Rancan, Dr. A. Klossek, K. Yamamoto, and Dr. R. Flesch. We thankfully acknowledge the Deutsche Forschungsgemeinschaft for the support of the Priority Program SPP 1313 “Biological Responses to Nanoscale Particles”, project RU420/12-1, and SFB 1112.

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Authors and Affiliations

  1. Department of Chemistry and Biotechnology, University of Applied Sciences Darmstadt, Stephanstrasse 7, 64295, Darmstadt, Germany

    Christina Graf

  2. Physical Chemistry, Institute of Chemistry and Biochemistry, Free University of Berlin, Takustrasse 3, 14195, Berlin, Germany

    Eckart Rühl

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  1. Christina Graf
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  2. Eckart Rühl
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Correspondence to Christina Graf or Eckart Rühl .

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  1. Institute of Anatomy, University of Bern, Bern, Switzerland

    Peter Gehr

  2. Institute of Physical Chemistry, University of Duisburg-Essen, Essen, Germany

    Reinhard Zellner

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Graf, C., Rühl, E. (2019). Imaging Techniques for Probing Nanoparticles in Cells and Skin. In: Gehr, P., Zellner, R. (eds) Biological Responses to Nanoscale Particles. NanoScience and Technology. Springer, Cham. https://doi.org/10.1007/978-3-030-12461-8_9

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