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Characterization of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids by Mass Spectrometry

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Book cover Bioactive Lipids in Health and Disease

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1127))

Abstract

Lipids containing unsaturated doubles bonds are oxidized by enzymatic and non-enzymatic mechanisms yielding hydroperoxides and hydroxides as primary products.

This process has been implicated in physiological and pathological mechanisms. Thus, precise characterization and quantification of lipid oxidation products in biological samples can provide important mechanistic insights. In this context, the use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to characterize the primary products of polyunsaturated fatty acids, like hydroxides and hydroperoxides, is a sensitive, specific and versatile tool. Here we will focus on the characterization and specific analysis of hydroxy and hydroperoxy regioisomers of linoleic acid, arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid by LC-MS/MS.

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Acknowledgments

The authors acknowledge the Brazilian research funding institutions Fundação de Amparo à Pesquisa do Estado de São Paulo [FAPESP, CEPID-Redoxoma 13/07937-8 and 10/50891-0]; Conselho Nacional de Desenvolvimento Científico e Tecnológico [CNPq, Universal 424094/2016-9]; NAP-Redoxoma; Pro-Reitoria de Pesquisa USP; and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [CAPES- Finance Code 001].

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  1. Priscilla Bento Matos Cruz Derogis

    Present address: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil

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  1. Departamento de Bioquímica, Instituto de Química – Universidade de São Paulo, São Paulo, SP, Brazil

    Priscilla Bento Matos Cruz Derogis, Adriano B. Chaves-Fillho & Sayuri Miyamoto

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  1. Priscilla Bento Matos Cruz Derogis
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Correspondence to Sayuri Miyamoto .

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  1. Departamento de Bioquímica and Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay

    Andres Trostchansky

  2. Departamento de Bioquímica and Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay

    Homero Rubbo

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Derogis, P.B.M.C., Chaves-Fillho, A.B., Miyamoto, S. (2019). Characterization of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids by Mass Spectrometry. In: Trostchansky, A., Rubbo, H. (eds) Bioactive Lipids in Health and Disease. Advances in Experimental Medicine and Biology, vol 1127. Springer, Cham. https://doi.org/10.1007/978-3-030-11488-6_2

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