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Neuronal Cultures and Nanomaterials

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In Vitro Neuronal Networks

Part of the book series: Advances in Neurobiology ((NEUROBIOL,volume 22))

Abstract

In recent years, the scientific community has witnessed an exponential increase in the use of nanomaterials for biomedical applications. In particular, the interest of graphene and graphene-based materials has rapidly risen in the neuroscience field due to the properties of this material, such as high conductivity, transparency and flexibility. As for any new material that aims to play a role in the biomedical area, a fundamental aspect is the evaluation of its toxicity, which strongly depends on material composition, chemical functionalization and dimensions. Furthermore, a wide variety of three-dimensional scaffolds have also started to be exploited as a substrate for tissue engineering. In this application, the topography is probably the most relevant amongst the various properties of the different materials, as it may allow to instruct and interrogate neural networks, as well as to drive neural growth and differentiation.

This chapter discusses the in vitro approaches, ranging from microscopy analysis to physiology measurements, to investigate the interaction of graphene with the central nervous system. Moreover, the in vitro use of three-dimensional scaffolds is described and commented.

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Author notes

  1. Fabio Benfenati and Fabrizia Cesca are the senior authors

Authors and Affiliations

  1. Center for Synaptic Neuroscience and Technology (NSYN@UniGe), Istituto Italiano di Tecnologia, Genova, Italy

    Mattia Bramini, Anna Rocchi, Fabio Benfenati & Fabrizia Cesca

  2. Graphene Labs, Istituto Italiano di Tecnologia, Genova, Italy

    Mattia Bramini, Fabio Benfenati & Fabrizia Cesca

  3. Department of Experimental Medicine, University of Genova, Genova, Italy

    Fabio Benfenati

Authors
  1. Mattia Bramini
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  2. Anna Rocchi
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  3. Fabio Benfenati
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  4. Fabrizia Cesca
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Corresponding authors

Correspondence to Mattia Bramini or Fabrizia Cesca .

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Editors and Affiliations

  1. Rehab Technologies IIT-INAIL Joint Lab, Istituto Italiano di Tecnologia, Genova, Italy

    Michela Chiappalone

  2. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy

    Valentina Pasquale

  3. Department of Clinical Neurophysiology, University of Twente, Enschede, The Netherlands

    Monica Frega

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Bramini, M., Rocchi, A., Benfenati, F., Cesca, F. (2019). Neuronal Cultures and Nanomaterials. In: Chiappalone, M., Pasquale, V., Frega, M. (eds) In Vitro Neuronal Networks. Advances in Neurobiology, vol 22. Springer, Cham. https://doi.org/10.1007/978-3-030-11135-9_3

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