Heterogeneity in Epiblast Stem Cells

  • Alice JouneauEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1123)


Epiblast stem cells (EpiSCs) are pluripotent cells that are derived from mouse embryos at gastrulation stages. They represent the primed state of pluripotency, in which cells are on the verge of differentiation and already express markers of the three primary lineages (mesoderm, endoderm, neurectoderm). EpiSCs display some heterogeneity intra- and inter-cell lines in the expression of some of these lineage markers. We relate this heterogeneity to signalling pathways that are active in EpiSCs, either due to addition of growth factors (FGF2 and activin) in the culture medium, or endogenously active (FGF, Nodal, and Wnt). By modulating Wnt or activin/nodal pathways, cell lines close to EpiSCs but with different properties can be obtained. These signalling pathways are all at work in vivo to pattern the pluripotent epiblast and specify cellular fates.


Epiblast Primed Pluripotency Heterogeneity Wnt Activin/Nodal EpiSC Differentiation Fate Signalling pathways Patterning 


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.UMR BDR, INRA, ENVA, Université Paris SaclayJouy en JosasFrance

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