Abstract
Within eukaryotic genomes, there are several types of small RNAs including sn, sno, si, and miRNAs. With respect to the Lemnaceae family, the vast majority of the research to date has been conducted in Spirodela polyrhiza, focused mainly on the miRNAs. This research consists of three small RNA-sequencing experiments in strains from China, Germany, and the USA, with each experiment identifying conserved miRNAs and predicting novel miRNAs and targets. While the novel miRNAs and recently discovered miRNAs fluctuated, the family size and expression of well-known miRNA families was consistent between the three experiments. While miRBase likely contains many incorrect annotations, these miRNAs were annotated according to strict criteria and analyzed for the miRBase high confidence list. They were further characterized through degradome sequencing, which confirmed half of the conserved miRNAs and a third of the novel. Finally, Spirodela polyrhiza has a surprisingly low abundance of 24nt sRNAs, which are required to suppress transposon proliferation.
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Acknowledgements
I would like to thank Professor Jie Tang of Chengdu University for providing data on his submitted and published work respectively and reviewing this manuscript to ensure its accuracy.
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Fourounjian, P. (2020). Small RNAs in Duckweeds. In: Cao, X., Fourounjian, P., Wang, W. (eds) The Duckweed Genomes. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-11045-1_16
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