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Real-Time PCR: Advanced Technologies and Applications

  • Güliz Uyar GüleçEmail author
  • Yaşar Barış Turgut
Chapter

Abstract

Fungal infections of central nervous system (CNS) are life-threatening diseases requiring early intervention. Fungi are ubiquitous in the environment, and known or emerging strains can be responsible for the infections. For rapid and reliable identification of fungal infections of the CNS, molecular methods are popular due to drawbacks of other diagnostic approaches. In particular, real-time polymerase chain reaction (PCR) has been used for identifying and quantifying of fungi. Different real-time PCR chemistries and technologies are in market. Species-specific and broad-range real-time PCR have been used successfully in clinical trials with high sensitivity. In conclusion, the most appropriate method should be chosen according to the clinical condition of patients and the facility of mycology laboratory.

Keywords

Central nervous system Diagnosis Fungal infection Real-time PCR 

Abbreviations

CNS

Central nervous system

CSF

Cerebrospinal fluid

dsDNA

Double-stranded DNA

FRET

Fluorescence resonance energy transfer

HIV

Human immunodeficiency virus

ITS

Internal transcribed spacer

PCR

Polymerase chain reaction

rDNA

Ribosomal DNA

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Deparment of Infectious Diseases and Clinical MicrobiologyAydın Adnan Menderes University School of MedicineAydınTurkey
  2. 2.Department of Internal MedicineMuğla Sıtkı Koçman University School of MedicineMuğlaTurkey

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