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Fungal Infections of the Central Nervous System pp 463–469Cite as

Real-Time PCR: Advanced Technologies and Applications

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Abstract

Fungal infections of central nervous system (CNS) are life-threatening diseases requiring early intervention. Fungi are ubiquitous in the environment, and known or emerging strains can be responsible for the infections. For rapid and reliable identification of fungal infections of the CNS, molecular methods are popular due to drawbacks of other diagnostic approaches. In particular, real-time polymerase chain reaction (PCR) has been used for identifying and quantifying of fungi. Different real-time PCR chemistries and technologies are in market. Species-specific and broad-range real-time PCR have been used successfully in clinical trials with high sensitivity. In conclusion, the most appropriate method should be chosen according to the clinical condition of patients and the facility of mycology laboratory.

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Abbreviations

CNS:

Central nervous system

CSF:

Cerebrospinal fluid

dsDNA:

Double-stranded DNA

FRET:

Fluorescence resonance energy transfer

HIV:

Human immunodeficiency virus

ITS:

Internal transcribed spacer

PCR:

Polymerase chain reaction

rDNA:

Ribosomal DNA

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Author information

Authors and Affiliations

  1. Deparment of Infectious Diseases and Clinical Microbiology, Aydın Adnan Menderes University School of Medicine, Aydın, Turkey

    Güliz Uyar Güleç

  2. Department of Internal Medicine, Muğla Sıtkı Koçman University School of Medicine, Muğla, Turkey

    Yaşar Barış Turgut

Authors
  1. Güliz Uyar Güleç
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  2. Yaşar Barış Turgut
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Corresponding author

Correspondence to Güliz Uyar Güleç .

Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Aydın Adnan Menderes University, School of Medicine, Aydın, Turkey

    Mehmet Turgut

  2. Department of Pathology, Basavatarakam Indo-American Hospital & Research Institute, Hyderabad, Telangana, India

    Sundaram Challa

  3. Department of Neurosurgery, Avicenne Military Hospital, Mohammed V University in Rabat, Marrakech, Morocco

    Ali Akhaddar

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Güleç, G.U., Turgut, Y.B. (2019). Real-Time PCR: Advanced Technologies and Applications. In: Turgut, M., Challa, S., Akhaddar, A. (eds) Fungal Infections of the Central Nervous System. Springer, Cham. https://doi.org/10.1007/978-3-030-06088-6_38

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  • DOI: https://doi.org/10.1007/978-3-030-06088-6_38

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-06087-9

  • Online ISBN: 978-3-030-06088-6

  • eBook Packages: MedicineMedicine (R0)

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