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Applications of Ion-Exchange Chromatography in Pharmaceutical Analysis

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Book cover Applications of Ion Exchange Materials in Biomedical Industries

Abstract

Ion-exchange chromatography represents a versatile way for the separation of highly polar (acidic and basic) compounds including pharmaceuticals, whereas the use of reversed-phase chromatography is not feasible. Symmetrical peaks with suitable retention times and exclusive selectivity were achieved using such technique. Compounds with strong UV absorption could be easily analyzed. However, for the analysis of substances lacking UV absorbance, several approaches have been followed to overcome the problem of using buffer salts (such as phosphate) which prevent the use of universal detectors. One of these approaches was the use of indirect UV detection. Alternatively, the mixed-mode column has been used in which volatile buffer solutions were applied; therefore, universal detectors can be applied. In addition to quantitative analysis, ion-exchange chromatography has been widely applied for sample purification or preconcentration steps prior to analysis.

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Abbreviations

DAD:

Diode array detector

ESI:

Electrospray ionization

HILIC:

Hydrophilic interaction liquid chromatography

MAX:

Mixed anion exchange

MCX:

Mixed cation exchange

MS:

Mass spectrometry

ODS:

Octadecylsilane

PBD-ZrO2:

Polybutadiene-coated zirconia

PGRP:

Positively charged reversed phase

RPLC:

Reversed-phase liquid chromatography

SAX:

Strong anion exchange

SCX:

Strong cation exchange

SPE:

Solid-phase extraction

UV:

Ultraviolet

WCX:

Weak cation exchange

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  1. Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt

    Sayed M. Derayea

  2. Pharmaceutical Analysis Department, Faculty of Pharmacy, Menoufia University, Shibin El Kom, Egypt

    Hytham M. Ahmed

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  1. Sayed M. Derayea
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  2. Hytham M. Ahmed
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Correspondence to Hytham M. Ahmed .

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  1. Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Inamuddin

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Derayea, S.M., Ahmed, H.M. (2019). Applications of Ion-Exchange Chromatography in Pharmaceutical Analysis. In: Inamuddin (eds) Applications of Ion Exchange Materials in Biomedical Industries. Springer, Cham. https://doi.org/10.1007/978-3-030-06082-4_5

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