Skip to main content
SpringerLink
Log in

Separation and Purification of Amino Acids

  • Chapter
  • First Online:
Book cover Applications of Ion Exchange Materials in Biomedical Industries

Abstract

The most prevalent type of liquid chromatography is ion exchange chromatography. In recent years, the acceptance of ion exchange chromatography has been increased because of its application in agricultural, pharmaceutical, environmental and biotechnology industries. Amino acids are principal components of proteins and their separation from protein hydrolysate or from a mixture of amino acids, is always in focus and ion exchange chromatography finds its application in separation of amino acids. Separation of amino acids by ion exchange chromatography has widespread applications because it has high capacity and it is easy to design. This chapter covers modern applications and basic principles of Ion exchange chromatography in the separation of amino acids.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Moustafa YM, Morsi RE (2013) Ion exchange chromatography-An overview. In: Column Chromatography. InTech

    Google Scholar 

  2. Singh C, Sharma C, Kamble P (2014) Amino acid analysis using ion-exchange chromatography: a review

    Google Scholar 

  3. Khan A, Faiz F (2008) Amino acid analysis using ion exchange resins. J Nat Sci Math 48(1–2):1–17

    CAS  Google Scholar 

  4. Yu H, Ding Y-S, Mou S-F, Jandik P, Cheng J (2002) Simultaneous determination of amino acids and carbohydrates by anion-exchange chromatography with integrated pulsed amperometric detection. J Chromatogr A 966(1):89–97

    Article  CAS  Google Scholar 

  5. Bhattacharyya L, Rohrer JS (2012) Applications of ion chromatography in the analysis of pharmaceutical and biological products. Wiley, London

    Google Scholar 

  6. Cascaval D, Oniscu C, Galaction A-I (2001) Selective separation of amino acids by reactive extraction. Biochem Eng J 7(3):171–176

    Article  CAS  Google Scholar 

  7. Casella IG, Contursi M (2003) Isocratic ion chromatographic determination of underivatized amino acids by electrochemical detection. Anal Chim Acta 478(2):179–189

    Article  CAS  Google Scholar 

  8. Casella IG, Gatta M, Cataldi TR (2000) Amperometric determination of underivatized amino acids at a nickel-modified gold electrode by anion-exchange chromatography. J Chromatogra A 878(1):57–67

    Article  CAS  Google Scholar 

  9. Ding Y, Yu H, Mou S (2002) Direct determination of free amino acids and sugars in green tea by anion-exchange chromatography with integrated pulsed amperometric detection. J Chromatogra A 982(2):237–244

    Article  CAS  Google Scholar 

  10. Petritis K, Elfakir C, Dreux M (2002) A comparative study of commercial liquid chromatographic detectors for the analysis of underivatized amino acids. J Chromatogra A 961(1):9–21

    Article  CAS  Google Scholar 

  11. Small H, Stevens TS, Bauman WC (1975) Novel ion exchange chromatographic method using conductimetric detection. Anal Chem 47(11):1801–1809

    Article  CAS  Google Scholar 

  12. Wiesel A, Schmidt-Traub H, Lenz J, Strube J (2003) Modelling gradient elution of bioactive multicomponent systems in non-linear ion-exchange chromatography. J Chromatogra A 1006(1–2):101–120

    Article  CAS  Google Scholar 

  13. Cummins PM, Dowling O, O’Connor BF (2011) Ion-exchange chromatography: basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase. In: Protein chromatography. Springer, Berlin, pp 215–228

    Google Scholar 

  14. Acikara OzB (2013) Ion-exchange chromatography and its applications. In: Column chromatography. InTech

    Google Scholar 

  15. Levison PR (2003) Large-scale ion-exchange column chromatography of proteins: comparison of different formats. J Chromatogra B 790(1–2):17–33

    Article  CAS  Google Scholar 

  16. Csapo J, Albert C, Loki K, Csapo-Kiss Z (2008) Separation and determination of the amino acids by ion exchange column chromatography applying postcolumn derivatization. Acta Universitatis Sapientiae Alimentaria 1:5–29

    Google Scholar 

  17. Csomos E, Simon-Sarkadi L (2002) Characterisation of Tokaj wines based on free amino acids and biogenic amines using ion-exchange chromatography. Chromatographia 56(1):S185–S188

    Article  CAS  Google Scholar 

  18. Yu H, Mou S-F (2006) Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography. J Chromatogra A 1118(1):118–124

    Article  CAS  Google Scholar 

  19. Hong SU, Bruening ML (2006) Separation of amino acid mixtures using multilayer polyelectrolyte nanofiltration membranes. J Membr Sci 280(1–2):1–5

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan

    Khush Bakhat Alia, Habibullah Nadeem, Ijaz Rasul, Farrukh Azeem & Muhammad Hussnain Siddique

  2. Department of Environmental Sciences and Engineering, Government College University, Faisalabad, Pakistan

    Sabir Hussain

  3. Department of Microbiology, Government College University, Faisalabad, Pakistan

    Saima Muzammil

  4. Department of Food Sciences, University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

    Muhammad Riaz

  5. Department of Zoology, Government College University, Faisalabad, Pakistan

    Shabab Nasir

Authors
  1. Khush Bakhat Alia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Habibullah Nadeem
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ijaz Rasul
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Farrukh Azeem
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sabir Hussain
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhammad Hussnain Siddique
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Saima Muzammil
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Muhammad Riaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shabab Nasir
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Habibullah Nadeem .

Editor information

Editors and Affiliations

  1. Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Inamuddin

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Alia, K.B. et al. (2019). Separation and Purification of Amino Acids. In: Inamuddin (eds) Applications of Ion Exchange Materials in Biomedical Industries. Springer, Cham. https://doi.org/10.1007/978-3-030-06082-4_1

Download citation

Publish with us

Policies and ethics

Search

Navigation