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Surface Enhanced Raman Spectroscopy for Biophysical Applications pp 57–83Cite as

Investigation on Nanoparticles and Their Molecular Functionalization

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Abstract

The essential building blocks of SERS-active systems we have studied are noble metal nanoparticles (Nps). They constitute the metallic substrate crucially contributing to SERS amplification thanks to the enhancement of the local electromagnetic field. Nowadays, Nps of various shape, size and material can be fabricated with a high degree of precision and their chemical properties can be tuned by employing different ligands. In this chapter, we will present the functionalization of noble metal Nps with a Raman reporter and their further conjugation with a biomolecule (folic acid). The characterization of the system with multiple techniques will be presented, and its SERS signature will be thoroughly discussed.

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Authors and Affiliations

  1. Dipartimento di Fisica e Geologia, Università degli Studi di Perugia, Perugia, Italy

    Claudia Fasolato

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  1. Claudia Fasolato
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Correspondence to Claudia Fasolato .

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Fasolato, C. (2018). Investigation on Nanoparticles and Their Molecular Functionalization. In: Surface Enhanced Raman Spectroscopy for Biophysical Applications. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-030-03556-3_3

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