Abstract
Bioprinting is the deposition of living cells , biomaterials , and biological molecules or factors in predesigned positions for the development of 3D bioengineering constructs. This chapter overviews 3D bioprinting techniques for the fabrication of scaffolds for tissue engineering application. Among the bioprinting techniques developed to date, extrusion-based bioprinting, which is based on a pneumatic or other mechanism to extrude or dispense materials and other biological molecules, has been widely used in the development of scaffolds . The basic principles of extrusion-based bioprinting are presented, the influence of bioprinting process parameters on scaffold fabrication is discussed, and methods for characterizing scaffold structures, including mechanical properties and porosities, are reviewed. Notably, cells incorporated in the bioprinting process can be injured due to process-induced mechanical forces , which may lead to cell damage and reduced cell viability and functioning after bioprinting. As such, this chapter also discusses the factors that cause cell damage as well as approaches that can be used to represent the percent cell damage.
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Chen, D.X.B. (2019). Extrusion Bioprinting of Scaffolds. In: Extrusion Bioprinting of Scaffolds for Tissue Engineering Applications. Springer, Cham. https://doi.org/10.1007/978-3-030-03460-3_6
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DOI: https://doi.org/10.1007/978-3-030-03460-3_6
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