Abstract
To print scaffolds , the selected biomaterials and associated biological elements, such as living cells and biomolecules, should be prepared in the form of a liquid or solution. More specifically, the biomaterial solutions or bioink must be prepared to have appropriate flow behaviors such that they can be extruded to form scaffolds with a 3D structure. As such, the preparation of biomaterial solutions is a critical step in printing tissue scaffolds with desired structures and functional properties. This chapter presents the preparation of solutions from various biomaterials for bioprinting and the methods/techniques used to characterize the flow behavior of prepared solutions.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
A. Skardal, A. Atala, Biomaterials for integration with 3-D bioprinting. Ann. Biomed. Eng. 43, 730–746 (2015)
A. Rajaram, D. Schreyer, D. Chen, Bioplotting alginate/hyaluronic acid hydrogel scaffolds with structural integrity and preserved schwann cell viability. 3D Printing Addit. Manuf. 1:194–203 (2014)
Z. Izadifar, T. Chang, W.M. Kulyk, D. Chen, B.F. Eames, Analyzing biological performance of 3D-printed, cell-impregnated hybrid constructs for cartilage tissue engineering. Tissue Eng. (2015)
S.V. Murphy, A. Atala, 3D bioprinting of tissues and organs. Nat. Biotechnol. 32, 773–785 (2014)
H.-W. Kang, S.J. Lee, I.K. Ko, C. Kengla, J.J. Yoo, A. Atala, A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat. Biotechnol. 34, 312–319 (2016)
F. Pati, J. Jang, D.-H. Ha, S.W. Kim, J.-W. Rhie, J.-H. Shim, et al., Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat. Commun. 5 (2014)
R. Landers, R. Mülhaupt, Desktop manufacturing of complex objects, prototypes and biomedical scaffolds by means of computer-assisted design combined with computer-guided 3D plotting of polymers and reactive oligomers. Macromol. Mater. Eng. 282, 17–21 (2000)
Y. Tabata, Tissue regeneration based on growth factor release. Tissue Eng. 9, 5–15 (2003)
W. Schuurman, V. Khristov, M. Pot, P. Van Weeren, W. Dhert, J. Malda, Bioprinting of hybrid tissue constructs with tailorable mechanical properties. Biofabrication. 3, 021001 (2011)
R.P. Chhabra, J.F. Richardson, Non-Newtonian flow in the process industries: fundamentals and engineering applications. Butterworth-Heinemann, UK (1999)
A. Rao, Rheology of fluid and semisolid foods: principles and applications. Springer Science & Business Media, Germany (2010)
P.J. Carreau, Rheological equations from molecular network theories. Trans. Soc. Rheology. 16, 99–127 (1972)
X. Chen, Dispensed-based bio-manufacturing scaffolds for tissue engineering applications. Int. J. Eng. Appl. (IREA). 2, 10–19 (2014)
X. Chen, Time-dependent rheological behavior of fluids for electronics packaging. J. Electron. Packag. 127, 370–374 (2005)
J. Aho, S. Syrjala, Determination of the entrance pressure drop in capillary rheometry using Bagley correction and zero-length capillary. Annu. Trans.-Nord. Rheol. Soc. 14, 143 (2006)
T. Billiet, E. Gevaert, T. De Schryver, M. Cornelissen, P. Dubruel, The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials 35, 49–62 (2014)
P. Vlasak, Z. Chara, Effect of particle size distribution and concentration on flow behavior of dense slurries. Part. Sci. Technol. 29, 53–65 (2011)
L, Ning, Y. Xu, X. Chen, D.J. Schreyer, Influence of mechanical properties of alginate-based substrates on the performance of Schwann cells in culture. J. Biomater. Sci. Polym. Ed. 1–33 (2016)
X. Tian, M. Li, N. Cao, J. Li, X. Chen, Characterization of the flow behavior of alginate/hydroxyapatite mixtures for tissue scaffold fabrication. Biofabrication 1, 045005 (2009)
H, Hamaker, The London—van der Waals attraction between spherical particles. physica. 4, 1058–1072 (1937)
G. Dimitreli, A.S. Thomareis, Effect of temperature and chemical composition on processed cheese apparent viscosity. J. Food Eng. 64, 265–271 (2004)
M.G. Li, X.Y. Tian, X. Chen, Temperature effect on the shear-induced cell damage in biofabrication. Artif. Organs 35, 741–746 (2011)
B.J. Alberts, A. Lewis, J. Raff, Molecular biology of the cell (2008)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Chen, D.X.B. (2019). Preparation of Scaffold Solutions and Characterization of Their Flow Behavior. In: Extrusion Bioprinting of Scaffolds for Tissue Engineering Applications. Springer, Cham. https://doi.org/10.1007/978-3-030-03460-3_5
Download citation
DOI: https://doi.org/10.1007/978-3-030-03460-3_5
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-03459-7
Online ISBN: 978-3-030-03460-3
eBook Packages: EngineeringEngineering (R0)