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Genetic Engineering of Carrot

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The Carrot Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

Carrot (Daucus carota) is one of the model species used in research for in vitro plant cell and tissue culture. The development of these techniques has enabled efficient cell and tissue proliferation and somatic embryogenesis under in vitro conditions, thus favoring the use of carrot for elucidating the mechanisms of horizontal gene transfer and gene function. Deployment of genetic engineering techniques has led to the development of carrots with improved traits, enhancing plant production for human health. The first product derived from genetically modified (GM) carrot cells cultured in a bioreactor has been approved for the treatment of human metabolic disease and for commercialization. This chapter describes methods of carrot genetic transformation using both vector and non-vector methods. Furthermore, we present reports of basic research in which carrot was used as a model to elucidate the function of heterologous genes and promoters, revealing selected mechanisms of plant metabolism, including the phenomenon of bacteria to plant gene transfer. Separate sections exemplify modified characteristics of GM carrot, including resistance to pathogens and the biosynthesis of recombinant proteins.

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Acknowledgements

The support of the Ministry of Science and Higher Education of the Republic of Poland is gratefully acknowledged.

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Authors and Affiliations

  1. Faculty of Biotechnology and Horticulture, Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425, Krakow, Poland

    Rafal Baranski & Aneta Lukasiewicz

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  1. Rafal Baranski
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  2. Aneta Lukasiewicz
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Correspondence to Rafal Baranski .

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Editors and Affiliations

  1. Vegetable Crops Research Unit, USDA-ARS, Madison, WI, USA

    Philipp Simon

  2. Plants for Human Health Institute, North Carolina State University , Kannapolis, NC, USA

    Massimo Iorizzo

  3. University of Agriculture in Krakow, Krakow, Poland

    Dariusz Grzebelus

  4. Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Krakow, Poland

    Rafal Baranski

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Baranski, R., Lukasiewicz, A. (2019). Genetic Engineering of Carrot. In: Simon, P., Iorizzo, M., Grzebelus, D., Baranski, R. (eds) The Carrot Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-03389-7_10

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