Skip to main content
SpringerLink
Log in

An Insight to Micropropagation of Freshwater Aquatic Medicinal Plants

  • Chapter
  • First Online:
Plant and Human Health, Volume 2

  • 1295 Accesses

Abstract

Freshwater aquatic plants include medicinal and ornamental species: have limited demand and rarely micropropagated. Most of them are found in Southeast Asian environments as amphibians or fully submerged or floating in lakes, streams, and watercourses that are ignored as weeds. These plants have never been central focuses and were not looked for characteristic proliferation. It is difficult to multiply these plants using traditional techniques through seeds or natural proliferation of rhizomes or cuttings by maintaining quality. Rare and fragmented but important information about micropropagation of these neglected plants has been discussed in this chapter. There are certain aquatic or semiaquatic medicinal plants for which no effort has been made to propagate in vitro. Contrarily, plants like water hyssop have extensive in vitro regeneration protocols due to its high demand. This study compares methodologies used by researchers to micropropagate these plants. It is concluded that this study will help in understanding and establishing systems for scientific propagation of these plants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Aasim M, Karataş M, Khawar KM, Dogan M (2013) Optimization of sterilization and micropropagation of water lettuce (Pistia stratiotes L.). J Appl Biol Sci 7:71–74

    CAS  Google Scholar 

  • Aasim M, Doğan M, Karataş M, Khawar KM (2017) In vitro whole plant regeneration of water lettuce (Pistia stratiotes L.) using Thidiazuron. J Glob Innov Agric Soc Sci 5:1–4

    Google Scholar 

  • Anu A, Nirmal Babu K, John CZ, Peter KV (2001) In vitro clonal multiplication of Acorus calamus L. J Plant Biochem Biotechnol 10:53–55

    Article  CAS  Google Scholar 

  • Bagadeka AN, Jayaraj M (2011) In vitro rhizogenesis from leaf and stem callus of Heliotropium indicum, L.-medicinal herb. IJPAES 1:1–5

    Google Scholar 

  • Barathi KK, Agastian P (2015) In vitro regeneration of a rare antidiabetic plant Epaltes divaricata L. South Indian J Biol Sci 1:52–59

    Article  Google Scholar 

  • Baskaran P, Jayabalan N (2005) An efficient micropropagation system for Eclipta alba, a valuable medicinal herb. In Vitro Cell Dev Biol Plant 41:532–539

    Article  CAS  Google Scholar 

  • Begum T, Mathur M (2014) In vitro regeneration of catharanthus roseus and Bacopa monnieri and their survey around Jaipur District. Int J Pure Appl Biosci 2:210–221

    Google Scholar 

  • Behera M, Mishra RR, Panigrahi J, Rath SP (2010) Micro-propagation of Astercantha longifolia (L.) Nees—an ethno-medicinal herb. Int J Genet Eng Biotechnol 1:141–148

    Google Scholar 

  • Behera S, Nayak N, Hota S, Barik DP, Naik SK (2015) An efficient micropropagation protocol of Bacopa monnieri (L.) Pennell through two-stage culture of nodal segments and ex vitro acclimatization. J Appl Biol Biotechnol 3:16–21

    Google Scholar 

  • Bhusari S, Wanjari R, Khobragade P (2013) Cost effective in vitro clonal propagation of Bacopa monnieri L. Pennel. Int J Indig Med Plant 46:1239–1244

    Google Scholar 

  • Bibi Y, Zia M, Nisa S, Habib D, Waheed A, Chaudhary FM (2011) Regeneration of Centella asiatica plants from non-embryogenic cell lines and evaluation of antibacterial and antifungal properties of regenerated calli and plants. J Biol Eng 5:13

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bisht S, Bisht NS, Bhandari S (2012) In vitro plant regeneration from seedling explants of Hedychium coronarium J. Koenig. J Med Plant Res 6:5546–5551

    Article  CAS  Google Scholar 

  • Chang X, Peng J, Di K, Na X, Yang JC (2011) Study on tissue cultivation and clone establishment of Centipeda minima. Special Wild Economic Animal and Plant Research. http://en.cnki.com.cn/Article_en/CJFDTOTAL-TCYA201104007.htm

  • Çinar A, Karataş M, Aasim M (2013) High frequency plant regeneration of dwarf hygro (Hygrophila polysperma [Roxb.] T. Anderson) on liquid culture. J Appl Biol Sci 7:75–78

    Google Scholar 

  • Dar RA, Koshy EP, Thomas G (2016) In vitro floral morphogenesis in Eclipta prostrata (L.). Asian J Biol Sci 2:49–51

    Article  Google Scholar 

  • Das D, Borua PK (2014) In vitro propagation of Alternanthera sessilis L. from internode explant. Br Biotechnol J 4:74–80

    Article  Google Scholar 

  • Das R, Hasan MF, Hossain MS, Rahman M (2008) Micropropagation of Centella asiatica L. an important medicinal herb. Prog Agric 19:51–56

    Google Scholar 

  • Dhaka N, Kothari SL (2005) Micropropagation of Eclipta alba, (L.) Hassk—an important medicinal plant. In Vitro Cell Dev Biol 41:658–661

    Article  Google Scholar 

  • Dixit H, Thakur A (2017) In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants. IJRPB 5:1–4

    Google Scholar 

  • Dixit V, Purshottam DK, Agnihotri P, Husain T, Misra P (2014) A highly efficient shoot organogenesis system of Acorus calamus L.—a threatened medicinal plant of Indian Himalaya. The Experiment 21:1453–1461

    Google Scholar 

  • Dogan M, Karataş M, Aasim M (2015) An efficient in vitro plantlet regeneration of Ceratophyllum demersum L., an important medicinal aquatic plant. Fresen Environ Bull 24(10):3499–3504

    CAS  Google Scholar 

  • Emsen B, Doğan M, Aasim M, Yildirim E (2016) Insecticidal activity of in vitro propagated aquatic plant Ceratophyllum demersum against granary weevil Sitophilus granarius L. (Coleoptera: Curcolionidae). Egypt J Biol Pest Control 26(3):619–624

    Google Scholar 

  • Gao M, Jiang W, Wei S, Lin Z, Cai B, Yang L, Luo C, He X, Tan J, Chen L (2015) High-efficiency propagation of Chinese water chestnut [Eleocharis dulcis (Burm.f.) Trin. ex Hensch] using a temporary immersion bioreactor system. Plant Cell Tissue Organ Cult 121:761–772

    Article  CAS  Google Scholar 

  • Gawde AJ, Paratkar GT (2004) Micropropagation of Eclipta alba Hassk: an approach to shorten the protocol. Indian J Biotechnol 3:128–132

    Google Scholar 

  • Ghasolia B, Shandilya D, Maheshwari R (2013) Multiple shoot regeneration of Bacopa monnieri (L.) using cyanobacterial media—a novel approach and effect of phytoregulators on in vitro micropropagation. Pelagia Res Lab 1:27–33

    Google Scholar 

  • Gnanaraj WE, Antonisamy JMA, Subramanian KM, Nallyan S (2011) Micropropagation of Alternanthera sessilis (L.) using shoot tip and nodal segments. Iran J Biotechnol 9:206–212

    CAS  Google Scholar 

  • Gonsalves J (2010) Economic botany and ethnobotany. Mittal Publications, New Delhi

    Google Scholar 

  • Gurnani C, Kumar V, Mukhija S, Dhingra A, Rajpurohit S, Narula P (2012) In vitro regeneration of brahmi (Bacopa monnieri (L.) Penn.)—a threatened medicinal plant. Kathmandu Univ J Sci Eng Technol 8:97–99

    Article  Google Scholar 

  • Harathi K, Naidu CV (2016) Influence of ethylene inhibitor silver nitrate on direct shoot regeneration from in vitro raised shoot tip explants of Sphaeranthus indicus Linn.—an important antijaundice medicinal plant. Am J Plant Sci 7:64894

    Article  CAS  Google Scholar 

  • Harathi K, Geetha G, Naidu CV (2016) Effect of silver nitrate and different carbon sources on in vitro shoot multiplication of Sphaeranthus indicus (Linn.)—an important antijaundice medicinal plant. Int J Pharm Biol Sci 6:185–192

    CAS  Google Scholar 

  • Hasan MF, Sikdar B (2010) In vitro propagation of Polygonum hydropiper L. from shoot tips. Plant Tissue Cult Biotechnol 20:73–79

    Article  Google Scholar 

  • Hassan AKMS, Begum N, Jahan MAA, Khatun R (2010) In vitro mass propagation of Heliotropium indicum L., using apical and axillary bud explants. Bangladesh J Sci Ind Res 45:69–74

    Article  CAS  Google Scholar 

  • Hettiarchchi A, Fernando KKS, Jayasuriya AHM (1997) In vitro propagation of Wadakha (Acorus calamus). J Natl Sci Council Sri Lanka 25:151–157

    Google Scholar 

  • Hoque A, Arima S (2004) Various color illumination effect on in vitro multiple shoot induction in water chestnut (Trapa japonica). Plant Tissue Cult 14:161–166

    Google Scholar 

  • Hoque A, Rahman SM, Arima S, Takagi Y (2001) Efficient in vitro germination and shoot proliferation of chilling treated water chestnut (Trapa japonica Flerov.) embryonal explants. In Vitro Cell Dev Biol 37:369–374

    Article  CAS  Google Scholar 

  • Hoque A, Nahar A, Razvy MA, Biswas MK, Kabir AH (2006) Micropropagation of water chestnut (Trapa sp.) through local varieties of Rajshahi Division. Asian J Plant Sci 5:409–413

    Article  Google Scholar 

  • Husain MK, Anis M (2006) Rapid in vitro propagation of Eclipta alba (L.) Hassk, through high frequency axillary shoot proliferation. Acta Physiol Plant 28:325–330

    Article  CAS  Google Scholar 

  • Jahirhussain G, Palanivel S, Tamilselvan V, Muniappan V, Deepa K, Veerappan R (2016) In vitro rapid multiplication of Coldenia procumbens L. from shoot tip explants. J Adv Appl Scient Res 1:18–28

    CAS  Google Scholar 

  • Jain A, Pandey K, Benjamin D, Kumar MA, Singh RK (2014) In vitro approach of medicinal herb: Bacopa monnieri. Int J Innov Res Sci Eng Technol 3:12088–12093

    Google Scholar 

  • Jenks MA, Kane ME, McConnell DB (2000) Shoot organogenesis from petiole explants in the aquatic plant Nymphoides indica. Plant Cell Tissue Organ Cult 63:1–8

    Article  CAS  Google Scholar 

  • Joshi AG, Ashutosh R, Pathak SAM, Singh S (2010) High frequency of shoot regeneration on leaf explants of Bacopa monnieri. Environ Exp Biol 8:81–84

    Google Scholar 

  • Joshi K, Chaturvedi P, Shubhpriya (2013) Efficient in vitro regeneration protocol of Centella asiatica (L.) urban: an endemic and underutilized nutraceutical herb. Afr J Biotechnol 12:5164–5172

    Article  CAS  Google Scholar 

  • Jun X et al (2011) Study on tissue culture and rapid propagation of Eleocharis dulcis. http://en.cnki.com.cn/Article_en/CJFDTOTAL-GNZL201106009.htm

  • Kane ME, Mcconnel DB, Sheehan TJ, Dehgan B (1988) A laboratory exercise to demonstrate adventitious shoot formation using stem internodes of parrot-feather. Hort Sci 23:408

    Google Scholar 

  • Kane ME, Gilman EF, Jenks MA, Sheehan TJ (1990) Micropropagation of the aquatic plant Cryptocoryne lucens. Hort Sci 25:687–689

    Google Scholar 

  • Karataş M, Aasim M (2014) Efficient adventitious shoot regeneration of medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell). Pak J Agric Sci 51:665–670

    Google Scholar 

  • Karataş M, Aasim M (2015a) In vitro plantlet regeneration from nodal segments of creeping jenny (Lysimachia nummularia L.)—a medicinal aquatic plant. Fresen Environ Bull 24:1263–1268

    Google Scholar 

  • Karataş M, Aasim M (2015b) In vitro whole plant regeneration of medicinal aquatic plant-Limnophila aromatica. Fresen Environ Bull 24:2747–2750

    Google Scholar 

  • Karataş M, Aasim M, Çinar A, Dogan M (2013a) Adventitious shoot regeneration from leaf explant of dwarf hygro (Hygrophila polysperma (Roxb.) T. Anderson). Scient World J 2013:680425. https://doi.org/10.1155/2013/680425

    Article  CAS  Google Scholar 

  • Karataş M, Aasim M, Dogan M, Khawar KM (2013b) Adventitious shoot regeneration of the medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell) using different internodes. Arch Biol Sci 65:297–303

    Article  Google Scholar 

  • Karataş M, Aasim M, Dogan M (2014a) Multiple shoot regeneration of Ceratophyllum demersum L. on agar solidified and liquid mediums. Fresen Environ Bull 24:3–9

    Google Scholar 

  • Karataş M, Aasim M, Çinar A (2014b) Adventitious shoot regeneration of dwarf hygro (Hygrophila Polysperma) under in vitro conditions. Fresen Environ Bull 23:2190–2194

    Google Scholar 

  • Karataş M, Aasim M, Çiftçioğlu M (2014c) Adventitious shoot regeneration of Roundleaf toothcup-Rotala rotundifolia [(Buch-Ham. ex Roxb) Koehne]. The JAPS 24:838–842

    Google Scholar 

  • Karataş M, Dogan M, Emsen B, Aasim M (2015) Determination of in vitro free radical scavenging activities of various extracts from in vitro propagated Ceratophyllum demersum L. Fresen Environ Bull 24:2946–2952

    Google Scholar 

  • Karataş M, Aasim M, Dogan M (2016a) Efficacy of in vitro propagated coontail (Ceratophyllum demersum L.) on quality of different water samples. Fresen Environ Bull 25:5113–5511

    Google Scholar 

  • Karataş M, Aasim M, Dazkirli M (2016b) Influence of light emitting diodes and benzylaminopurine on adventitious shoot regeneration of water hyssop (Bacopa monnieri L. Pennel.) in vitro. Arch Biol Sci 68:501–508

    Article  Google Scholar 

  • Kasselmann C (1999) Piante d’acquario. Primaris, Rozzano

    Google Scholar 

  • Katiyar SK, Chandel G (1998) High frequency somatic embryogenesis from immature inflorescence of Coix aquatica Roxb. Plant Tissue Cult 8:131–137

    Google Scholar 

  • Kirdmanee C, Phaephun W, Teerakathiti T, Suriyan Cha-Um S, Takagi M (2006) An effective in-vitro selection of water spinach (Ipomoea aquatica Forsk.) for NaCI-, KH2PO4- and temperature-stresses. Environ Control Biol 44:265–277

    Article  Google Scholar 

  • Koul A, Sharma A, Gupta S, Mallubhotla S (2014) Cost effective protocol for micropropagation of Bacopa monnieri using leaf explants. IJSR 3:210–212

    Google Scholar 

  • Kumar MS, Nandi SC (2015) High frequency plant regeneration with histological analysis of organogenic callus from internode explants of Asteracantha longifolia Nees. J Genet Eng Biotechnol 13:31–37

    Article  Google Scholar 

  • Kumar MS, Rao MV (2007) In vitro micropropagation of Heliotropium indicum Linn.—an Ayurvedic herb. Indian J Plant Biotechnol 6:245–249

    CAS  Google Scholar 

  • Marc JM, Hagendoorn MJM, Geelen TAM, Beek TAV, Jamar DCL, Tetteroo FAA, van der Pla LHW (1994) Occurrence of polygodial in plant organs and tissue culture of Polygonum hydropiper. Physiol Plant 92:595–600

    Article  Google Scholar 

  • Masanori F, Atsuhiko N, Kazuya Y (1997) Methods for introducing foreign genes into tropical aquatica plant Ipomoea aquatica and regenerating the plant. Patent CA section 3

    Google Scholar 

  • Mehta J, Kumar V, Syedy M, Upadhyay D, Ansari R, Bisht V, Naaz H, Soni P (2012) In vitro shoot regeneration of Bacopa monnieri (L.) using cyanobacterial media—a novel approach and effect of phytoregulators on in vitro micropropagation. Asian J Plant Sci Res 2:699–706

    CAS  Google Scholar 

  • Micheli M, De Gasperis A, Prosperi F, Standardi A (2006) Micropropagation of three species of aquatic plants. Agric Med 1–6. https://www.researchgate.net/publication/236655811_Micropropagation_of_three_species_of_aquatic_plants

  • Mishra RR, Behera M, Kumar DR, Panigrahi J (2006) High frequency regeneration of plantlets form seedling explants of Astercantha longifolia (L.) Nees. J Plant Biotechnol 8:27–35

    Google Scholar 

  • Mochida K, Tsujimoto H (2001) Production of wheat doubled haploids by pollination with Job's tears (Coix lacryma-Jobi L.). J Hered 92:81–83

    Article  CAS  PubMed  Google Scholar 

  • Mohanta YK, Sahoo S (2014) In vitro culture of highly valuable medicinal plant Bacopa monnieri (L.) Penn. for rapid and mass multiplication. Int J Pharm Sci Invent 3:41–45

    CAS  Google Scholar 

  • Mohanty P, Behera S, Swain SS, Barik DP, Naik SK (2013) Micropropagation of Hedychium coronarium J. Koenig through rhizome bud. Physiol Mol Biol Plants 19:605–610

    Article  PubMed  PubMed Central  Google Scholar 

  • Mohapatra H, Barik DP, Rath SP (2008) In vitro regeneration of medicinal plant Centella asiatica. Biol Plant 52:339

    Article  CAS  Google Scholar 

  • Naik PM, Patil BR, Kotagi KS, Kazi AM, Lokesh H, Kamplikoppa SG (2014) Rapid one step protocol for in vitro regeneration of Bacopa monnieri (L.). J Cell Tissue Res 14:4293–4296

    CAS  Google Scholar 

  • Pal PP, Arefin MB, Banerjee N (2014) In vitro regeneration of Hygrophila schulli (Buch. Ham) M. R. & S.M. Almeida through callus mediated somatic embryogenesis. Int J Curr Res 6:5997–6002

    Google Scholar 

  • Pandiyan P, Selvaraj T (2012) In vitro multiplication of Bacopa monnieri (L.) Pennell from shoot tip and nodal explants. J Agric Technol 8:1099–1108

    CAS  Google Scholar 

  • Panigrahi J, Mishra RR, Behera M (2006) In vitro multiplication of Astercantha longifolia (L.) Nees—a medicinal herb. Indian J Biotechnol 5:562–564

    CAS  Google Scholar 

  • Panthalu CS, Mahadev MD, Naidu CV (2014) High efficiency adventitious indirect organogenesis and plant regeneration from callus of Centella asiatica (L.)—an important Antijaundice medicinal plant. IJAR 2:1027–1036

    Google Scholar 

  • Parida R, Mohanty S, Nayak S (2013) In vitro propagation of Hedychium coronarium Koen. through axillary bud proliferation. Plant Biosyst 147:905–912

    Article  Google Scholar 

  • Pierik RLM, Ruibing MA (1997) Developments in the micropropagation industry in the Netherlands. Plant Tissue Cult Biotechnol 3:152–153

    Google Scholar 

  • Prasad NK, Prasad MS, Aradhya SM, Shivamurthy GR (2006) Callus induction from Ipomoea aquatica Forsk. Leaf and its antioxidant activity. Indian J Biotechnol 5:107–111

    CAS  Google Scholar 

  • Priyadarshini M, Kumari R, Anjali K, Arunimal, Prasad S, Shukla LN (2014a) Tissue culture studies of Heliotropium indicum an important medicinal herb for callus induction and micro propagation. In: Third world conference on applied sciences, engineering & technology 27–29 September 2014, Kathmandu, Nepal

    Google Scholar 

  • Priyadarshini M, Kumari R, Shukla LN (2014b) Development of protocol for efficient callusing in Heliotropium indicum L. an important medicinal herb. GJABHS 2:37–42

    Google Scholar 

  • Ravi S, Muthukumar B, Natarajan E, Ramesh Kannan N, Vijayalatha KR (2012) Plant regeneration and in vitro flowering from nodal explants of Hydrolea zeylanica—a rare medicinal plant. Plant Cell Biol Mol Biol 13:147–150

    Google Scholar 

  • Ravipaul SCB, Jawahar M, Asleelan MJ (2008) Micropropagation of Sphaeranthus amaranthoides Burm. F.—a multipurpose medicinal herb. Int J Biol Chem Sci 2:579–586

    Google Scholar 

  • Rout JR, Sahoo SL, Ray SS, Sethi BK, Das R (2011) Standardization of an efficient protocol for in vitro clonal propagation of Bacopa monnieri L.—an important medicinal plant. J Agric Technol 7:289–299

    Google Scholar 

  • Roy A, Kundu K, Saxena G, Kumar L, Bharadvaja N (2016) Effect of different media and growth hormones on shoot multiplication of in vitro grown Centella asiatica accessions. Adv Tech Biol Med 4:172

    Google Scholar 

  • Sen MK, Hassan MM, Nasrin S, Jamal MAHM, Mamun-Or-Rashid AMN, Biswas N (2013) An efficient plant regeneration protocol for Achyranthes aspera L. IRJOB 4:94–100

    Google Scholar 

  • Shahidullah AKM (2007) The role of medicinal plants in livelihood improvement and ecological sustainability in Bangladesh, Thesis, Manitoba University

    Google Scholar 

  • Sharan AK, Dubey SR, Kumar R, Chandra V, Singh BP, Kumar G, Kumari S (2014) A novel protocol for propagation of Eclipta alba (L.) Hassak. Int Pure Appl Biosci 2:137–141

    Google Scholar 

  • Sharma A, Bhansali S, Kumar A (2013) In vitro callus induction and shoot regeneration in Eclipta alba (L.) HASSK. Int J Life Sci Pharma Res 3:43–46

    CAS  Google Scholar 

  • Sharma N, Singh R, Pandey R (2016) In vitro propagation and conservation of Bacopa monnieri L. Methods Mol Biol 1391:153–171

    Article  CAS  PubMed  Google Scholar 

  • Shekhawat MS, Manokari M (2015) Direct organogenesis from rhizome explants in Marsilea quadrifolia L.: a threatened fern species. Adv Biol 2015:639678

    Article  CAS  Google Scholar 

  • Shekhawat MS, Manokari M, Revathi J (2017) In vitro propagation, micromorphological studies and ex vitro rooting of Alternanthera philoxeroides (Mart.) Griseb.: an important aquatic plant. Aquacult Int 25:423

    Article  Google Scholar 

  • Singh A, Kandasamy T, Odhav B (2009) In vitro propagation of Alternanthera sessilis (sessile joyweed), a famine food plant. Afr J Biotechnol 8:5691–5695

    Article  CAS  Google Scholar 

  • Singh SK, Rai MK, Sahoo L (2012) An improved and efficient micropropagation of Eclipta alba through transverse thin cell layer culture and assessment of clonal fidelity using RAPD analysis. Ind Crop Prod 37:328–333

    Article  CAS  Google Scholar 

  • Sivakumar G, Alagumanian S, Rao MV (2006) High frequency in vitro multiplication of Centella asiatica: an important industrial medicinal herb. Eng Life Sci 6:597–601

    Article  CAS  Google Scholar 

  • Staritski G (1977) Die vitrokultur von Cryptocoryne. Aqua Planta 1:3–6

    Google Scholar 

  • Stodola J (1980) Le piante d’acquario. Olimpia Ed, Firenze

    Google Scholar 

  • Subashri B, Koilpillai YJ (2013) High frequency regeneration of Bacopa monnieri plant callus derived from internode. Int J Pharm Bio Sci 4(1):263–266

    CAS  Google Scholar 

  • Tiwari C, Bakshi M, Vichitra A (2013) A rapid two step protocol of in vitro propagation of an important medicinal herb Centella asiatica Linn. Afr J Biotechnol 12:1084–1090

    CAS  Google Scholar 

  • Verma M, Bansal YK (2013) Effect of additives on plant regeneration in Hedychium coronarium J. Koenig an endangered aromatic and medicinal herb. Int J Pharm Sci Rev Res 23:105–110

    CAS  Google Scholar 

  • Verma M, Bansal YK (2014a) Effect of a potent cytokinin Thidiazuron (TDZ) on in vitro regeneration of Hedychium coronarium J. Koenig—a valuable medicinal plant. Int J Rec Biotechnol 2:38–44

    Google Scholar 

  • Verma M, Bansal YK (2014b) Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig. Indian J Exp Biol 50:904–909

    Google Scholar 

  • Verma S, Singh N (2012) In vitro mass multiplication of Acorus calamus L.—an endangered medicinal plant. Am Eurasian J Agric Environ Sci 12:1514–1521

    CAS  Google Scholar 

  • Vijay R, Shukla J, Rajesh Saxena R (2016) Propagation of Bacopa monnieri (BRAHMI): important medicinal plant. CIB Tech J Biotechnol 5:17–23

    Google Scholar 

  • Vijayakumar M, Vijayakumar R, Stephen R (2010) In vitro propagation of Bacopa monnieri L.—a multipurpose plant. Indian J Sci Technol 3:781–786

    CAS  Google Scholar 

  • Wangdi K, Sarethy IP (2016) Evaluation of micropropagation system of Bacopa monnieri L. in liquid culture and its effect on antioxidant properties. J Herb Spice Med Plant 22:69–80

    Article  CAS  Google Scholar 

  • Yarra R, Aileni M, Vemunoori AK, Kokkirala VR, Umate P, Abbagani S (2010) Direct shoot regeneration from mature leaf explants of Sphaeranthus indicus L., a multipurpose medicinal plant. J Phytol 2:05–11

    Google Scholar 

  • Yesmin S, Hashem A, Islam MS (2015) Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk. Jahangirnagar Univ J Biol Sci 4:61–69

    Article  Google Scholar 

  • Zhang Y, Wang Y, Yang B, Chen S (2008) In vitro regeneration and propagation of Pistia stratiotes: an ideal aquatic plant for biomanufacturing and bioremediation. Chin J Appl Environ Biol 14:445–449

    Google Scholar 

Download references

Acknowledgment

Authors are thankful to Ms. Ecenur Korkmaz and Ms. Areeza Emman for their kind efforts and help to improve the quality of work.

Author information

Authors and Affiliations

  1. Faculty of Science, Department of Biotechnology, Necmettin Erbakan University, Konya, Turkey

    Muhammad Aasim & Mehmet Karataş

  2. Faculty of Agriculture, Department of Field Crops, Ankara University, Ankara, Turkey

    Khalid Mahmood Khawar

  3. Faculty of Agricultural and Natural Science, Department of Field Crops, Abant Izzet Baysal University, Bolu, Turkey

    Faheem Shahzad Bloch

  4. Faculty of Agricultural Sciences and Technologies, Department of Agricultural Genetic Engineering, Nigde University, Nigde, Turkey

    Allah Bakhsh

Authors
  1. Muhammad Aasim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Khalid Mahmood Khawar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mehmet Karataş
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Faheem Shahzad Bloch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Allah Bakhsh
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Botany and Centre for Environmental Studies Ege University, Vice President of the Islamic World Academy of Sciences Amann, Jordan, Izmir, Izmir, Turkey

    Munir Ozturk

  2. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Khalid Rehman Hakeem

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Aasim, M., Khawar, K.M., Karataş, M., Bloch, F.S., Bakhsh, A. (2019). An Insight to Micropropagation of Freshwater Aquatic Medicinal Plants. In: Ozturk, M., Hakeem, K. (eds) Plant and Human Health, Volume 2. Springer, Cham. https://doi.org/10.1007/978-3-030-03344-6_19

Download citation

Publish with us

Policies and ethics

Search

Navigation