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Pericytes Derived from Human Pluripotent Stem Cells

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Pericyte Biology - Novel Concepts

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1109))

Abstract

Pericytes wrap blood microvessels and are believed to play important roles in vascular morphogenesis, maturation, and stability. In addition, pericytes have emerged as candidates for targeting cancer growth and for wound healing. In order to model these processes and test new therapies, it is desirable to have a reliable, scalable source of pericytes. Human pluripotent stem cells (hPSCs), which possess the ability to differentiate into any cell type in the body, have been used to generate pericytes in vitro quickly, consistently, and with high yields. In this chapter, we consider the differentiation of pericytes from hPSCs. We compare the approaches taken by multiple groups and discuss characterization of hPSC-pericytes. Studying pericyte differentiation in vitro provides the opportunity to identify factors influencing pericyte development and to establish the ontogenic relationships between pericytes and similar cells. The development of highly specific, defined pericyte populations from hPSCs will enable downstream applications requiring large quantities of cells, including tissue engineered models and cell therapies.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Institute for NanoBioTechnology, and the Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD, USA

    John Jamieson, Bria Macklin & Sharon Gerecht

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  1. John Jamieson
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  2. Bria Macklin
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  3. Sharon Gerecht
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Correspondence to Sharon Gerecht .

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Editors and Affiliations

  1. Department of Radiology, Columbia University Medical Center, New York, NY, USA

    Alexander Birbrair

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Jamieson, J., Macklin, B., Gerecht, S. (2018). Pericytes Derived from Human Pluripotent Stem Cells. In: Birbrair, A. (eds) Pericyte Biology - Novel Concepts. Advances in Experimental Medicine and Biology, vol 1109. Springer, Cham. https://doi.org/10.1007/978-3-030-02601-1_9

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