Abstract
The accumulations of chemical pesticides residues in soils represent one of the greatest risks for the environment and especially for plants. The copper bioviability in rhizosphere is the clearest aspect of this pollution process, causing high levels of metal stress, reducing crops yield by altering physiological and biochemical processes in plants. To face such risks, researchers have frequently studied the effect of plant growth promoting rhizobacteria (PGPR) as enhancer of the plant growth under abiotic stress. The goal of this study was firstly to know the level of broad bean tolerance to metal stress of copper, and its physiological effects in the plant, on the one hand, and the role of plant-Pseudomonas interaction in enhancing the plant tolerance level to metal stress, on the other. In this study, three Pseudomonas strains were isolated and screened by salinity and copper tolerance, then they were individually used as an inoculum in rhizosphere of broad bean Vicia faba (OTONO variety) in the presence of 0; 2.5; 10 and 20 mM.L-1 of CuSO4. According to the obtained results, under copper stress conditions with and without bacterial inoculation, the production of biomass and total chlorophyll content were significantly decreased. Copper treatments increased proline content in inoculated plants with P7 and P15 strains and in those which were not inoculated. However, this content was decreased in inoculated plants with P1 strain. The inoculation with P1, P7 and P15 strains motivated the production of fresh biomass and accumulation of proline, and otherwise decreased total chlorophyll content in plants.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Alia, M.P., Matysik, J.: Effect of proline on the production of singlet oxygen. Amino Acids 21, 191–203 (2001)
Andreazza, R., Pieniz, S., Okeke, B.C., Camargo, F.A.O.: Evaluation of copper resistant bacteria from vineyard soils and mining waste for copper biosorption. Braz. J. Microbiol. (BJM-1469) (2010)
Chen, W.N., Wu, C.H., James, E.K., Chang, J.S.: Metal biosorption capability of Cupriavidus taiwanensis and its effects on heavy metal removal by nodulated Mimosa pudica. J. Hazard. Mater. 151, 364–371 (2008)
Chen, C.T., Chen, L.M., Lin, C.C., Kao, C.H.: Regulation of proline accumulation in detached rice leaves exposed to excess copper. Plant Sci. 160, 283–290 (2001)
Chugh, L.K., Sawhney, S.K.: Photosynthetic activities of Pisum sativum seedlings grown in presence of cadmium. Plant Physiol. Biochem. 37, 297–303 (1999)
Cook, C.M., Kostidou, A., Vardaka, E., Lanaras, T.: Effects of copper on the growth, photosynthesis and nutrient concentrations of Phaseolus plants. Photosynthetica 34(2), 179–193 (1997)
Dell’Amico, E., Cavalca, L., Andreoni, V.: Improvement of Brassica napus growth under cadmium stress by cadmium resistant rhizobacteria. Soil Biol. Biochem. 40, 74–84 (2008)
Egamberdiyeva, D.: The effect of plant growth promoting bacteria on growth and utrient uptake of maize in two different soils. Appl. Soil. Ecol. 36, 184–189 (2007)
Ibrahim, A.S.S., El-Tayeb, M.A., Elbadawi, Y.B., Al-Salamah, A.A.: Bioreduction of Cr (vi) by potent novel chromate resistant alkaliphilic Bacillus sp. Strain KSUCr5 isolated from hypersaline soda lakes. Afric. J. Biotechnol. 10, 7207–7218 (2011)
Kavi, K.P.B., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu, K.R., Rao, K.R.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu, N.: Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr. Sci. 88, 424–438 (2005)
Kumar, K.V., Singh, N., Behl, H.M., Srivastava, S.: Influence of plant growth promoting bacteria and its mutant on heavy metal toxicity in Brassica juncea grown in fly ash amended soil. Chemosphere 72, 678–683 (2008)
Lewis, S., Donkin, M.E., Depledge, M.H.: Hsp70 expression in Enteromorpha intestinalis (Chlorophyta) exposed to environmental stressors. Aquat. Toxicol. 51, 277 (2001)
Li, Y.M., Chaney, R., Brewer, E., Rosenberg, R., Angle, S.J., Baker, A.J.M., Reeves, R.D.: Nelkin J Development of technology for commercial phytoextraction of nickel: economic and technical considerations. Plant Soil 249, 107–115 (2003)
Ma, Y., Rajkumar, M., Freitas, H.: Inoculation of plant growth promoting bacterium Achromobacter xylosoxidans strain Ax10 for the improvement of copper phytoextraction by Brassica juncea. J. Environ. Manage. 90, 831–837 (2009)
Miller, G., Stein, H., Honing, A., Kapulnik, Y., Zilberstein, A.: Responsive modes of Medicago sativa proline dehdrogenase genes during salt and recovery dictate free proline accumulation. Planta 222, 70–79 (2005)
Oliveira, T., Mucha, A.P., Reis, I., Rodrigues, P., Gomes, C.R., Almeida, C.M.R.: Copper phytoremediation by a salt marsh plant (Phragmites australis) enhanced by autochthonous bioaugmentation. Mar. Pollut. Bull. https://doi.org/10.1016/j.marpolbul.2014.08.038 (2014)
Oves, M., Khan, M.S., Zaidi., A.: Chromium reducing and plant growth promoting novel strain Pseudomonas aeruginosa OSG41 enhance chickpea growth in chromium amended soils. Eur. J. Soil Biol. 56:72–83 (2013)
Pätsikkä, E., Kairavuo, M., Sersen, F., Aro, E.-M., Tyystjärvi, E.: Excess copper predisposes photosystem II to photoinhibition in vivo by outcompeting iron and causing decrease in leaf chlorophyll. Plant Physiol. 129(3), 1359–1367 (2002)
Sheng, M., Tang, M., Chen, H., Yang, B., Zhang, F., Huang, Y.: Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18, 287–296 (2008)
Zarea, M.J., Hajinia, S., Karimi, N., Mohammadi, Goltapeh E., Rejali, F., Varma, A.: Effect of Piriformospora indica and Azospirillum strains from saline or non-saline soil on mitigation of the effects of NaCl. Soil Biol. Biochem. 45, 139–146 (2012)
Zengin, F.K., Kirbag, S.: Effects of copper on chlorophyll, proline, protein and abscisic acid level of sunflower (Helianthus Annuus L.) seedlings. J. Environ. Biol. 28(3), 561–566 (2007)
Zhang, L.P., Mehta, S.K., Liu, Z.P., Yang, A.Z.: Copper-induced proline synthesis is associated with nitric oxide generation in Chlamydomonas reinhardtii. Plant Cell Physiol. 49(3), 411–419 (2008)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this paper
Cite this paper
Idder, B., Djibaoui, R., Reguieg Yassaad El Hocine, A., Djoudi, A. (2019). Effects of Inoculation with Rhizospheric Pseudomonas on Physiological Responses in the Broad Bean (Vicia Faba) Grown Under Copper Stress. In: Chenchouni, H., Errami, E., Rocha, F., Sabato, L. (eds) Exploring the Nexus of Geoecology, Geography, Geoarcheology and Geotourism: Advances and Applications for Sustainable Development in Environmental Sciences and Agroforestry Research. CAJG 2018. Advances in Science, Technology & Innovation. Springer, Cham. https://doi.org/10.1007/978-3-030-01683-8_14
Download citation
DOI: https://doi.org/10.1007/978-3-030-01683-8_14
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-01682-1
Online ISBN: 978-3-030-01683-8
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)