Abstract
The aim of the research was to found grape breeding method that allows obtaining virus-free plants during breeding. It used the apical meristem for grapes breeding which is usually free from viruses. The conducted observations showed that a part of meristems was necrotic during the first stage of cultivation (2 weeks). The remaining meristems have evolved into micropiles of 2–2.5 mm which were re-transplanted. One month after planting, the surviving apical meristems were transplanted into a nutrient medium containing the same components. The transplantation was carried out in biological test tubes performed during 45–55 days for formation of regenerants with a size of 6–10 cm and obtaining microclones. The obtained material quality was measured according to growth regulators implemented such as 6-benzylaminopurine (6-BAP) and gibberellic acid (GA). It was found the microshoots grown on a medium with GA and 6-BAP concentration of 0.5 mg/l developed the most effective and virus free grape plants. 40% of the survival rate of the apical meristems was reached which allowed their further cultivation and propagation aiming at the production of virus-free planting material.
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References
Batukaev A.A.: Advances in technology of accelerated reproduction and improving the grape plant material by in vitro method, pp. 1–222. Publishing house of Moscow Timiryazev Agricultural Academy, Moscow (1998). (In Russian)
Burgutin A.B., Butenko R.G., Kataeva N.V., Golodriga P.Y.: Rapid clonal propagation of a grape plant. Agric. Biol. 7, 48–50 (1983). (In Russian)
Doroshenko, N.P.: Increase in the regenerative capacity of meristems in obtaining the virus-free grape material. Grapes Wine Russia 2, 6–9 (1997). (In Russian)
Garre, M., Martin-Tanguy, J., Mussillon, P.: La cultur de meristemes et la multilpication Végetative in Vitro au service de la pepiniere. Bull. Petits Fruit 14, 7–65 (1979)
Gamburg, K.Z., Rekoslavskaya, N.I., Shevtsov, S.G.: Auxins in Plant Tissue and Cell Culture, pp. 243. Novossibirsk, Nauka (1990). (In Russian)
Kulaeva, O.N.: Cytokinins, Their Structure and Functions, pp. 264. Moscow, Nauka (1973). (In Russian)
Vysotskii, V.A.: Biotechnological methods in modern gardening, vol. 26, pp. 3–10. Collection of research papers of All-Russian Research Institute of Horticultural Crops Selection, Moscow (2011). (In Russian)
Acknowledgements
The results were obtained in the framework of the RFBR no. 16-35-60051, Ministry of Education and Science of the Russian Federation No. 6.6222.2017/8.9, President of Russian Federation no. MК-3476.2017.5.
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Batukaev, A., Mukailov, M., Batukaev, M., Minkina, T., Sushkova, S. (2019). A Biotechnological Method for Breeding Grapes Using in Vitro Growth Stimulants. In: Chenchouni, H., Errami, E., Rocha, F., Sabato, L. (eds) Exploring the Nexus of Geoecology, Geography, Geoarcheology and Geotourism: Advances and Applications for Sustainable Development in Environmental Sciences and Agroforestry Research. CAJG 2018. Advances in Science, Technology & Innovation. Springer, Cham. https://doi.org/10.1007/978-3-030-01683-8_13
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DOI: https://doi.org/10.1007/978-3-030-01683-8_13
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