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Dysfunctional Corneal Endothelium: Delivery of Cell Therapy

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Corneal Regeneration

Part of the book series: Essentials in Ophthalmology ((ESSENTIALS))

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Abstract

Cellular therapies are an emerging alternative to allogeneic corneal transplantation for endothelial dysfunction. While allogeneic transplantation is limited by donor tissue supply, current culture systems are able to produce sufficient cells from a single donor cornea to treat multiple patients. A variety of delivery systems have been developed to harness the therapeutic potential of these cultured cells. This chapter outlines these delivery techniques, including use of single cell monolayers taken directly from culture plates, implantation of cell-seeded scaffolds, both biologic and synthetic, and injection of cells into the anterior chamber.

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References

  1. Tan DT, Dart JK, Holland EJ, et al. Corneal transplantation. Lancet. 2012;379(9827):1749–61.

    Article  Google Scholar 

  2. Ang M, Mehta JS, Lim F, et al. Endothelial cell loss and graft survival after Descemet’s stripping automated endothelial keratoplasty and penetrating keratoplasty. Ophthalmology. 2012;119(11):2239–44.

    Article  PubMed  Google Scholar 

  3. Price MO, Fairchild KM, Price DA, et al. Descemet’s stripping endothelial keratoplasty five-year graft survival and endothelial cell loss. Ophthalmology. 2011;118(4):725–9.

    Article  PubMed  Google Scholar 

  4. Guerra FP, Anshu A, Price MO, et al. Descemet’s membrane endothelial keratoplasty: prospective study of 1-year visual outcomes, graft survival, and endothelial cell loss. Ophthalmology. 2011;118(12):2368–73.

    Article  PubMed  Google Scholar 

  5. Ang M, Wilkins MR, Mehta JS, et al. Descemet membrane endothelial keratoplasty. Br J Ophthalmol. 2016b;100(1):15–21.

    Article  PubMed  Google Scholar 

  6. Eye Bank Association of America. 2010; 2009 Eye Banking Statistical Report.

    Google Scholar 

  7. Eye Bank Association of America. 2016; 2015 Eye Banking Statistical Report.

    Google Scholar 

  8. Gain P, Jullienne R, He Z, et al. Global survey of corneal transplantation and eye banking. JAMA Ophthalmol. 2016;134(2):167–73.

    Article  PubMed  Google Scholar 

  9. Baum JL, Niedra R, Davis C, et al. Mass culture of human corneal endothelial cells. Arch Ophthalmol. 1979;97(6):1136–40.

    Article  CAS  PubMed  Google Scholar 

  10. Peh GS, Beuerman RW, Colman A, et al. Human corneal endothelial cell expansion for corneal endothelium transplantation: an overview. Transplantation. 2011;91(8):811–9.

    Article  PubMed  Google Scholar 

  11. Honda N, Mimura T, Usui T, et al. Descemet stripping automated endothelial keratoplasty using cultured corneal endothelial cells in a rabbit model. Arch Ophthalmol. 2009;127(10):1321–6.

    PubMed  Google Scholar 

  12. Okumura N, Sakamoto Y, Fujii K, et al. Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction. Sci Rep. 2016b;6:26113.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Koizumi N, Sakamoto Y, Okumura N, et al. Cultivated corneal endothelial cell sheet transplantation in a primate model. Invest Ophthalmol Vis Sci. 2007;48(10):4519–26.

    Article  PubMed  Google Scholar 

  14. Koizumi N, Sakamoto Y, Okumura N, et al. Cultivated corneal endothelial transplantation in a primate: possible future clinical application in corneal endothelial regenerative medicine. Cornea. 2008;27(Suppl 1):S48–55.

    Article  PubMed  Google Scholar 

  15. Maurice DM, Giardini AA. Swelling of the cornea in vivo after the destruction of its limiting layers. Br J Ophthalmol. 1951;35(12):791–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Han SB, Ang H, Balehosur D, et al. A mouse model of corneal endothelial decompensation using cryoinjury. Mol Vis. 2013;19:1222–30.

    PubMed  PubMed Central  Google Scholar 

  17. Wu M, Hu ZL, Sun XM, et al. In vivo confocal microscopic evaluation of corneal endothelial dysfunction induced by phacoemulcification in rhesus monkey models. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2016;38(1):42–8.

    PubMed  Google Scholar 

  18. Zhang W, Hu Y, Lu L, et al. Rabbit model of corneal endothelial injury established using the Nd: YAG laser. Cornea. 2017;36(10):1274–81.

    PubMed  Google Scholar 

  19. Ang M, Konstantopoulos A, Goh G, et al. Evaluation of a micro-optical coherence tomography for the corneal endothelium in an animal model. Sci Rep. 2016a;6:29769.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Kim do K, Sim BR, Khang G. Nature-derived Aloe Vera gel blended silk fibroin film scaffolds for cornea endothelial cell regeneration and transplantation. ACS Appl Mater Interfaces. 2016;8(24):15160–8.

    Article  PubMed  CAS  Google Scholar 

  21. Mimura T, Shimomura N, Usui T, et al. Magnetic attraction of iron-endocytosed corneal endothelial cells to Descemet’s membrane. Exp Eye Res. 2003;76(6):745–51.

    Article  CAS  PubMed  Google Scholar 

  22. Mimura T, Yamagami S, Usui T, et al. Long-term outcome of iron-endocytosing cultured corneal endothelial cell transplantation with magnetic attraction. Exp Eye Res. 2005a;80(2):149–57.

    Article  CAS  PubMed  Google Scholar 

  23. Niu G, Choi JS, Wang Z, et al. Heparin-modified gelatin scaffolds for human corneal endothelial cell transplantation. Biomaterials. 2014;35(13):4005–14.

    Article  CAS  PubMed  Google Scholar 

  24. Okumura N, Koizumi N, Ueno M, et al. ROCK inhibitor converts corneal endothelial cells into a phenotype capable of regenerating in vivo endothelial tissue. Am J Pathol. 2012;181(1):268–77.

    Article  CAS  PubMed  Google Scholar 

  25. Zhou Y, Wu Z, Ge J, et al. Development and characterization of acellular porcine corneal matrix using sodium dodecylsulfate. Cornea. 2011;30(1):73–82.

    Article  PubMed  Google Scholar 

  26. Tuft SJ, Williams KA, Coster DJ. Endothelial repair in the rat cornea. Invest Ophthalmol Vis Sci. 1986;27(8):1199–204.

    CAS  PubMed  Google Scholar 

  27. Werner L, Chew J, Mamalis N. Experimental evaluation of ophthalmic devices and solutions using rabbit models. Vet Ophthalmol. 2006;9(5):281–91.

    Article  PubMed  Google Scholar 

  28. Van Horn DL, Sendele DD, Seideman S, et al. Regenerative capacity of the corneal endothelium in rabbit and cat. Invest Ophthalmol Vis Sci. 1977;16(7):597–613.

    PubMed  Google Scholar 

  29. Kimoto M, Shima N, Yamaguchi M, et al. Development of a bioengineered corneal endothelial cell sheet to fit the corneal curvature. Invest Ophthalmol Vis Sci. 2014;55(4):2337–43.

    Article  CAS  PubMed  Google Scholar 

  30. Bostan C, Theriault M, Forget KJ, et al. In vivo functionality of a corneal endothelium transplanted by cell-injection therapy in a feline model. Invest Ophthalmol Vis Sci. 2016;57(4):1620–34.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Van Horn DL, Hyndiuk RA. Endothelial wound repair in primate cornea. Exp Eye Res. 1975;21(2):113–24.

    Article  PubMed  Google Scholar 

  32. Hsiue GH, Lai JY, Chen KH, et al. A novel strategy for corneal endothelial reconstruction with a bioengineered cell sheet. Transplantation. 2006;81(3):473–6.

    Article  CAS  PubMed  Google Scholar 

  33. Sumide T, Nishida K, Yamato M, et al. Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces. FASEB J. 2006;20(2):392–4.

    Article  CAS  PubMed  Google Scholar 

  34. Madathil BK, Kumar PR, Kumary TV. N-isopropylacrylamide-co-glycidylmethacrylate as a thermoresponsive substrate for corneal endothelial cell sheet engineering. Biomed Res In. 2014;2014:450672.

    Article  CAS  Google Scholar 

  35. Teichmann J, Valtink M, Gramm S, et al. Human corneal endothelial cell sheets for transplantation: thermo-responsive cell culture carriers to meet cell-specific requirements. Acta Biomater. 2013;9(2):5031–9.

    Article  CAS  PubMed  Google Scholar 

  36. Tsaousis KT, Kopsachilis N, Tsinopoulos IT, et al. In vitro study of the deturgescence ability of cultivated human corneal endothelial cells. Cornea. 2016;35(5):669–72.

    Article  PubMed  Google Scholar 

  37. Lai JY, Lu PL, Chen KH, et al. Effect of charge and molecular weight on the functionality of gelatin carriers for corneal endothelial cell therapy. Biomacromolecules. 2006;7(6):1836–44.

    Article  CAS  PubMed  Google Scholar 

  38. Lai JY, Li YT. Functional assessment of cross-linked porous gelatin hydrogels for bioengineered cell sheet carriers. Biomacromolecules. 2010;11(5):1387–97.

    Article  CAS  PubMed  Google Scholar 

  39. Lai JY, Ma DH, Lai MH, et al. Characterization of cross-linked porous gelatin carriers and their interaction with corneal endothelium: biopolymer concentration effect. PLoS One. 2013;8(1):e54058.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Dua HS, Azuara-Blanco A. Amniotic membrane transplantation. Br J Ophthalmol. 1999;83(6):748–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Fan T, Ma X, Zhao J, et al. Transplantation of tissue-engineered human corneal endothelium in cat models. Mol Vis. 2013;19:400–7.

    CAS  PubMed  PubMed Central  Google Scholar 

  42. Ishino Y, Sano Y, Nakamura T, et al. Amniotic membrane as a carrier for cultivated human corneal endothelial cell transplantation. Invest Ophthalmol Vis Sci. 2004;45(3):800–6.

    Article  PubMed  Google Scholar 

  43. Zhang C, Nie X, Hu D, et al. Survival and integration of tissue-engineered corneal stroma in a model of corneal ulcer. Cell Tissue Res. 2007;329(2):249–57.

    Article  PubMed  Google Scholar 

  44. Bayyoud T, Thaler S, Hofmann J, et al. Decellularized bovine corneal posterior lamellae as carrier matrix for cultivated human corneal endothelial cells. Curr Eye Res. 2012;37(3):179–86.

    Article  CAS  PubMed  Google Scholar 

  45. Du L, Wu X, Pang K, et al. Histological evaluation and biomechanical characterisation of an acellular porcine cornea scaffold. Br J Ophthalmol. 2011;95(3):410–4.

    Article  PubMed  Google Scholar 

  46. Fu Y, Fan X, Chen P, et al. Reconstruction of a tissue-engineered cornea with porcine corneal acellular matrix as the scaffold. Cells Tissues Organs. 2010;191(3):193–202.

    Article  CAS  PubMed  Google Scholar 

  47. Ju C, Gao L, Wu X, et al. A human corneal endothelium equivalent constructed with acellular porcine corneal matrix. Indian J Med Res. 2012;135(6):887–94.

    CAS  PubMed  PubMed Central  Google Scholar 

  48. Yoeruek E, Bayyoud T, Maurus C, et al. Decellularization of porcine corneas and repopulation with human corneal cells for tissue-engineered xenografts. Acta Ophthalmol. 2012;90(2):e125–31.

    Article  PubMed  Google Scholar 

  49. Amano S, Shimomura N, Yokoo S, et al. Decellularizing corneal stroma using N2 gas. Mol Vis. 2008;14:878–82.

    CAS  PubMed  PubMed Central  Google Scholar 

  50. Sasaki S, Funamoto S, Hashimoto Y, et al. In vivo evaluation of a novel scaffold for artificial corneas prepared by using ultrahigh hydrostatic pressure to decellularize porcine corneas. Mol Vis. 2009;15:2022–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  51. Pang K. Differentiation of human embryonic stem cells to corneal epithelium and endothelium like cells for cornea replacement construction. Invest Ophth Vis Sci. 2015;56:5831.

    Article  CAS  Google Scholar 

  52. Bloomfield P, Wheatley DJ, Prescott RJ, et al. Twelve-year comparison of a Bjork-Shiley mechanical heart valve with porcine bioprostheses. N Engl J Med. 1991;324(9):573–9.

    Article  CAS  PubMed  Google Scholar 

  53. Booth C, Korossis SA, Wilcox HE, et al. Tissue engineering of cardiac valve prostheses I: development and histological characterization of an acellular porcine scaffold. J Heart Valve Dis. 2002;11(4):457–62.

    PubMed  Google Scholar 

  54. He Z, Forest F, Bernard A, et al. Cutting and decellularization of multiple corneal stromal lamellae for the bioengineering of endothelial grafts. Invest Ophthalmol Vis Sci. 2016;57(15):6639–51.

    Article  PubMed  Google Scholar 

  55. Peh GSL, Ang HP, Lwin CN, et al. Regulatory compliant tissue-engineered human corneal endothelial grafts restore corneal function of rabbits with bullous keratopathy. Sci Rep. 2017;7(1):14149.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  56. Jumblatt MM, Maurice DM, Schwartz BD. A gelatin membrane substrate for the transplantation of tissue cultured cells. Transplantation. 1980;29(6):498–9.

    Article  CAS  PubMed  Google Scholar 

  57. Mohay J, Lange TM, Soltau JB, et al. Transplantation of corneal endothelial cells using a cell carrier device. Cornea. 1994;13(2):173–82.

    Article  CAS  PubMed  Google Scholar 

  58. Lu PL, Lai JY, Ma DH, et al. Carbodiimide cross-linked hyaluronic acid hydrogels as cell sheet delivery vehicles: characterization and interaction with corneal endothelial cells. J Biomater Sci Polym Ed. 2008;19(1):1–18.

    Article  PubMed  Google Scholar 

  59. Watanabe R, Hayashi R, Kimura Y, et al. A novel gelatin hydrogel carrier sheet for corneal endothelial transplantation. Tissue Eng Part A. 2011;17(17–18):2213–9.

    Article  CAS  PubMed  Google Scholar 

  60. Kabosova A, Azar DT, Bannikov GA, et al. Compositional differences between infant and adult human corneal basement membranes. Invest Ophthalmol Vis Sci. 2007;48(11):4989–99.

    Article  PubMed  Google Scholar 

  61. Merrett K, Fagerholm P, McLaughlin CR, et al. Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: performance of type I versus type III collagen. Invest Ophthalmol Vis Sci. 2008;49(9):3887–94.

    Article  PubMed  Google Scholar 

  62. Fagerholm P, Lagali NS, Ong JA, et al. Stable corneal regeneration four years after implantation of a cell-free recombinant human collagen scaffold. Biomaterials. 2014;35(8):2420–7.

    Article  CAS  PubMed  Google Scholar 

  63. Levis HJ, Peh GS, Toh KP, et al. Plastic compressed collagen as a novel carrier for expanded human corneal endothelial cells for transplantation. PLoS One. 2012;7(11):e50993.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. Hayes S, Lewis P, Islam MM, et al. The structural and optical properties of type III human collagen biosynthetic corneal substitutes. Acta Biomater. 2015;25:121–30.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Kim EY, Tripathy N, Cho SA, et al. Bioengineered neo-corneal endothelium using collagen type-I coated silk fibroin film. Colloids Surf B Biointerfaces. 2015;136:394–401.

    Article  CAS  PubMed  Google Scholar 

  66. Madden PW, Lai JN, George KA, et al. Human corneal endothelial cell growth on a silk fibroin membrane. Biomaterials. 2011;32(17):4076–84.

    Article  CAS  PubMed  Google Scholar 

  67. Chen J, Yan C, Zhu M, et al. Electrospun nanofibrous SF/P(LLA-CL) membrane: a potential substratum for endothelial keratoplasty. Int J Nanomedicine. 2015;10:3337–50.

    CAS  PubMed  PubMed Central  Google Scholar 

  68. McLaughlin CR, Acosta MC, Luna C, et al. Regeneration of functional nerves within full thickness collagen-phosphorylcholine corneal substitute implants in guinea pigs. Biomaterials. 2010;31(10):2770–8.

    Article  CAS  PubMed  Google Scholar 

  69. Ozcelik B, Brown KD, Blencowe A, et al. Ultrathin chitosan-poly(ethylene glycol) hydrogel films for corneal tissue engineering. Acta Biomater. 2013;9(5):6594–605.

    Article  CAS  PubMed  Google Scholar 

  70. Wang TJ, Wang IJ, Chen S, et al. The phenotypic response of bovine corneal endothelial cells on chitosan/polycaprolactone blends. Colloids Surf B Biointerfaces. 2012a;90:236–43.

    Article  CAS  PubMed  Google Scholar 

  71. Wang TJ, Wang IJ, Lu JN, et al. Novel chitosan-polycaprolactone blends as potential scaffold and carrier for corneal endothelial transplantation. Mol Vis. 2012b;18:255–64.

    CAS  PubMed  PubMed Central  Google Scholar 

  72. Wang TJ, Wang IJ, Hu FR, et al. Applications of biomaterials in corneal endothelial tissue engineering. Cornea. 2016;35(Suppl 1):S25–30.

    Article  PubMed  Google Scholar 

  73. Salehi S, Bahners T, Gutmann J, et al. Characterization of structural, mechanical and nano-mechanical properties of electrospun PGS/PCL fibers. RSC Adv. 2014a;4(33):16951–7.

    Article  CAS  Google Scholar 

  74. Salehi S, Fathi M, Javanmard SH, et al. Generation of PGS/PCL blend nanofibrous scaffolds mimicking corneal stroma structure. Macromol Mater Eng. 2014b;299(4):455–69.

    Article  CAS  Google Scholar 

  75. Salehi S, Czugala M, Stafiej P, et al. Poly (glycerol sebacate)-poly (epsilon-caprolactone) blend nanofibrous scaffold as intrinsic bio- and immunocompatible system for corneal repair. Acta Biomater. 2017;50:370–80.

    Article  CAS  PubMed  Google Scholar 

  76. Rizwan M, Peh GS, Ang HP, et al. Sequentially-crosslinked bioactive hydrogels as nano-patterned substrates with customizable stiffness and degradation for corneal tissue engineering applications. Biomaterials. 2017;120:139–54.

    Article  CAS  PubMed  Google Scholar 

  77. McCulley JP, Maurice DM, Schwartz BD. Corneal endothelial transplantation. Ophthalmology. 1980;87(3):194–201.

    Article  CAS  PubMed  Google Scholar 

  78. Patel SV, Bachman LA, Hann CR, et al. Human corneal endothelial cell transplantation in a human ex vivo model. Invest Ophthalmol Vis Sci. 2009;50(5):2123–31.

    Article  PubMed  Google Scholar 

  79. Mimura T, Yokoo S, Araie M, et al. Treatment of rabbit bullous keratopathy with precursors derived from cultured human corneal endothelium. Invest Ophthalmol Vis Sci. 2005b;46(10):3637–44.

    Article  PubMed  Google Scholar 

  80. Mimura T, Yamagami S, Usui T, et al. Necessary prone position time for human corneal endothelial precursor transplantation in a rabbit endothelial deficiency model. Curr Eye Res. 2007;32(7–8):617–23.

    Article  PubMed  Google Scholar 

  81. Clement O, Siauve N, Cuenod CA, et al. Liver imaging with ferumoxides (Feridex): fundamentals, controversies, and practical aspects. Top Magn Reson Imaging. 1998;9(3):167–82.

    Article  CAS  PubMed  Google Scholar 

  82. Wang YX, Hussain SM, Krestin GP. Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging. Eur Radiol. 2001;11(11):2319–31.

    Article  CAS  PubMed  Google Scholar 

  83. Moysidis SN, Alvarez-Delfin K, Peschansky VJ, et al. Magnetic field-guided cell delivery with nanoparticle-loaded human corneal endothelial cells. Nanomedicine. 2015;11(3):499–509.

    Article  CAS  PubMed  Google Scholar 

  84. Bi YL, Wu MF, Lu LX, et al. Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles. Mol Med Rep. 2013;7(6):1767–72.

    Article  CAS  PubMed  Google Scholar 

  85. Okumura N, Ueno M, Koizumi N, et al. Enhancement on primate corneal endothelial cell survival in vitro by a ROCK inhibitor. Invest Ophthalmol Vis Sci. 2009;50(8):3680–7.

    Article  PubMed  Google Scholar 

  86. Garnock-Jones KP. Ripasudil: first global approval. Drugs. 2014;74(18):2211–5.

    Article  CAS  PubMed  Google Scholar 

  87. Okumura N, Kakutani K, Inoue R, et al. Generation and feasibility assessment of a new vehicle for cell-based therapy for treating corneal endothelial dysfunction. PLoS One. 2016a;11(6):e0158427.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  88. Adamis AP, Filatov V, Tripathi BJ, et al. Fuchs’ endothelial dystrophy of the cornea. Surv Ophthalmol. 1993;38(2):149–68.

    Article  CAS  PubMed  Google Scholar 

  89. Rizwan M, Peh GS, Adnan K, et al. In vitro topographical model of Fuchs dystrophy for evaluation of corneal endothelial cell monolayer formation. Adv Healthc Mater. 2016;5(22):2896–910.

    Article  CAS  PubMed  Google Scholar 

  90. Lange TM, Wood TO, McLaughlin BJ. Corneal endothelial cell transplantation using Descemet’s membrane as a carrier. J Cataract Refract Surg. 1993;19(2):232–5.

    Article  CAS  Google Scholar 

  91. Vazquez N, Rodriguez-Barrientos CA, Aznar-Cervantes SD, et al. Silk fibroin films for corneal endothelial regeneration: transplant in a rabbit Descemet membrane endothelial Keratoplasty. Invest Ophthalmol Vis Sci. 2017;58(9):3357–65.

    Article  CAS  PubMed  Google Scholar 

  92. Mimura T, Yamagami S, Yokoo S, et al. Cultured human corneal endothelial cell transplantation with a collagen sheet in a rabbit model. Invest Ophthalmol Vis Sci. 2004;45(9):2992–7.

    Article  PubMed  Google Scholar 

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Compliance Statements

J. S. Mehta, M. Lovatt, G. Peh, and S. Wahlig declare that they have no conflict of interest. All procedures performed by the authors followed and were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study. All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of Interest: No conflicting relationship exists for any author.

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Authors and Affiliations

  1. Tissue Engineering and Stem Cell Group, The Academia, Singapore Eye Research Institute, Singapore, Singapore

    Stephen Wahlig, Gary Swee-Lim Peh & Matthew Lovatt

  2. Singapore National Eye Centre, Department of Cornea and External Disease, Singapore, Singapore

    Jodhbir S. Mehta

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  1. Stephen Wahlig
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  2. Gary Swee-Lim Peh
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Editors and Affiliations

  1. Miguel Hernández University , Alicante, Spain

    Jorge L. Alió

  2. Vissum-Instituto Oftalmologico de Alica, University Miguel Hernandez, Alicante, Alicante, Spain

    Jorge L. Alió del Barrio

  3. Vissum Corporation, Madrid, Spain

    Francisco Arnalich-Montiel

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Wahlig, S., Peh, G.SL., Lovatt, M., Mehta, J.S. (2019). Dysfunctional Corneal Endothelium: Delivery of Cell Therapy. In: Alió, J., Alió del Barrio, J., Arnalich-Montiel, F. (eds) Corneal Regeneration . Essentials in Ophthalmology. Springer, Cham. https://doi.org/10.1007/978-3-030-01304-2_33

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