Cell-based Therapy Using Induced Plutipotent Stem Cell

  • Ricardo Pedro Casaroli-MaranoEmail author
Part of the Essentials in Ophthalmology book series (ESSENTIALS)


Advanced cell-based therapy has seen important advances over the last decade. An emerging alternative source of cells for the treatment of ocular disease is adult tissue progenitor cells. Despite certain limitations about complete terminal differentiation, they benefit from high proliferative potential, lower immunogenicity, and being easy to obtain by minimally invasive techniques. Induced pluripotent stem cells (IPSCs) are an additional alternative that have unlimited translational potential in organ and tissue regeneration. From the first investigations into cell reprogramming, which induced a full state of cellular pluripotency, to the most recent approaches for direct reprogramming through non-integrative methodology, advances in the understanding of events related to cellular proliferation and differentiation for clinical purposes has improved considerably. Several research groups are working on innovative methods and techniques to obtain different cellular types of ocular tissue, including epithelial and endothelial cells of the cornea, neurosensory retina, and pigmentary epithelium, using cell differentiation from IPSC lines or cells in partial states of induced pluripotency. However, the large-scale application of IPSC lines is not yet possible because drawbacks related to their clinical applicability need to be resolved, such as the tolerability and safety of receptor tissue and the need to develop refined clinical grade protocols for their production and differentiation. This chapter is intended to present some of the main innovations and future perspectives concerning the clinical applicability of IPSCs in ocular pathology, focusing on the ocular surface pathology.


Regenerative medicine Cell reprogramming Differentiation Limbal stem cells Limbal stem cell deficiency Adult tissue stem cells Advanced therapy Cell-based therapy Corneal epithelial cells 



The author acknowledges support from Fundació Marató TV3 (120630-31) and Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III (FIS14-PI00196 and FIS18-PI00355), from the European Regional Development Fund (FEDER) of European Union.

Compliance with ethical requirements: Ricardo Pedro Casaroli-Marano declares that he has no conflict of interest. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study. No animal studies were carried out by the authors for this article.


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Authors and Affiliations

  1. 1.Department of Surgery, School of Medicine and Hospital Clínic de BarcelonaUniversity of BarcelonaBarcelonaSpain
  2. 2.Institute of Biomedical Research (IIB-Sant Pau) and Barcelona Tissue BankBanc de Sang i TeixitsBarcelonaSpain

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