Cell Therapy Using Ex Vivo Cultured Limbal Cells: CLET and Equivalent

  • Paolo RamaEmail author
  • Giulio Ferrari
Part of the Essentials in Ophthalmology book series (ESSENTIALS)


Cell therapy for treatment of corneal lesions, with associated corneal (limbal) stem cell deficiency due to ocular burns, has recently gained regulatory approval from the European Medicines Agency (EMA), and it is the first choice to restore corneal surface integrity when corneal epithelial stem cells have been lost. LSCD can be due to a number of genetic, inflammatory, or traumatic disorders and frequently results in mono- or binocular blindness. In this chapter, we will review different limbal stem cell therapies for LSCD. We will do so by considering the epidemiology of LSCD and different types of cultivated limbal stem cell treatments. Alternative treatments will also be briefly reviewed. Potential advantages, and pitfalls, of autologous versus allogenic limbal stem cell transplantation will be considered.


Corneal stem cell Stem cell deficiency Advanced therapies Ocular surface burns Corneal epithelium 


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.San Raffaele Hospital, Department of Ophthalmology—Cornea and Ocular SurfaceMilanItaly

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