One Cell, Two Phenotypes: Capturing Pluripotency for Corneal Regeneration

  • Trevor SherwinEmail author
  • Carol Ann Greene
  • Colin R. Green
  • Kushant R. Kapadia
Part of the Essentials in Ophthalmology book series (ESSENTIALS)


A major goal for corneal restoration and regeneration over the last decade has been the production of bioengineered corneas in vitro for transplant, with many research laboratories investigating a variety of scaffold materials aimed at preserving the physiological and optical properties of the cornea. However, advances in cell reprogramming and in gene therapy have made the possibility of in vivo corneal engineering a distinct possibility. Here we describe the methods by which we have been able to induce in vivo keratocytes to produce proteins normally associated with other cell phenotypes, which may prove to be simple and effective examples of in vivo corneal engineering.


Corneal regeneration Keratocyte reprogramming Keratocyte pluripotency 


Compliance with Ethical Requirements

Carol Greene, Colin Green and Trevor Sherwin are coinventors listed on patent PCT/NZ2016/050033, application date: 05 Mar 2015. Kushant Kapadia has no conflict of interest to declare. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study. All institutional and national guidelines for the care and use of laboratory animals were followed.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Trevor Sherwin
    • 1
    Email author
  • Carol Ann Greene
    • 1
  • Colin R. Green
    • 1
  • Kushant R. Kapadia
    • 1
  1. 1.Department of Ophthalmology, New Zealand National Eye Centre, Faculty of Medical and Health SciencesThe University of AucklandAucklandNew Zealand

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