One Cell, Two Phenotypes: Capturing Pluripotency for Corneal Regeneration
A major goal for corneal restoration and regeneration over the last decade has been the production of bioengineered corneas in vitro for transplant, with many research laboratories investigating a variety of scaffold materials aimed at preserving the physiological and optical properties of the cornea. However, advances in cell reprogramming and in gene therapy have made the possibility of in vivo corneal engineering a distinct possibility. Here we describe the methods by which we have been able to induce in vivo keratocytes to produce proteins normally associated with other cell phenotypes, which may prove to be simple and effective examples of in vivo corneal engineering.
KeywordsCorneal regeneration Keratocyte reprogramming Keratocyte pluripotency
Compliance with Ethical Requirements
Carol Greene, Colin Green and Trevor Sherwin are coinventors listed on patent PCT/NZ2016/050033, application date: 05 Mar 2015. Kushant Kapadia has no conflict of interest to declare. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study. All institutional and national guidelines for the care and use of laboratory animals were followed.
- 1.Chen Z, You J, Liu X, Cooper S, Hodge C, Sutton G, Crook JM, Wallace GG. Biomaterials for corneal bioengineering. Biomed Mater. Accepted Manuscript online 12 October 2017.Google Scholar
- 6.Hendrix MJ, Hay ED, von der Mark K, Linsenmayer TF. Immunohistochemical localization of collagen types I and II in the developing chick cornea and tibia by electron microscopy. Invest Ophthalmol Vis Sci. 1982;22(3):359–75.Google Scholar
- 14.Manificat H, Rovère MR, Galiacy SD, Malecaze F, Hulmes DJ, Moali C, Damour O. Development of ex vivo organ culture models to mimic human corneal scarring. Mol Vis. 2012;18:2896–908. Epub 2012 Dec 1.Google Scholar
- 28.Kapadia K. Safety of a novel in vivo corneal tissue regeneration therapy for Keratoconus. Master of Science in Biomedical Science Thesis; 2017.Google Scholar