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Role of the Unconventional Prefoldin Proteins URI and UXT in Transcription Regulation

  • Phillip A. Thomas
  • Paolo Mita
  • Susan Ha
  • Susan K. LoganEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1106)

Abstract

The Unconventional prefoldin RPB5 interacting protein (URI), also known as RPB5-Mediating Protein (RMP) has been shown to play several regulatory roles in different cellular compartments including the mitochondria, as a phosphatase binding protein; in the cytoplasm, as a chaperone-like protein; and in the nucleus, as a transcriptional regulator through binding to RPB5 and RNA polymerase II (polII). This chapter focuses on the role URI plays in transcriptional regulation in the prostate cell. In prostate cells, URI is tightly bound to another prefoldin-like protein called UXT, a known androgen receptor (AR) cofactor. Part of a multiprotein complex, URI and UXT act as transcriptional repressors, and URI regulates KAP1 through PP2A phosphatase activity. The discovery of the interaction of URI and UXT with KAP1, AR, and PP2A, as well as the numerous interactions between URI and components of the R2TP/prefoldin-like complex, RPB5, and nuclear proteins involved in DNA damage response, chromatin remodeling and gene transcription, reveal a pleiotropic effect of the URI/UXT complex on nuclear processes. The mechanisms by which URI/UXT affect transcription, chromatin structure and regulation, and genome stability, remain to be elucidated but will be of fundamental importance considering the many processes affected by alterations of URI/UXT and other prefoldins and prefoldin-like proteins.

Keywords

Unconventional prefoldin RPB5 interacting protein (URI) Ubiquitously Expressed Transcript (UXT) Androgen Receptor Trapped clone-27 (ART-27) Androgen Receptor (AR) Gene transcription, prostate cancer Proteomics KRAB-associated protein 1 (KAP1) Protein phosphatase PP2A Retroelements Long Interspersed Element-1 (LINE-1) 

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Phillip A. Thomas
    • 1
  • Paolo Mita
    • 2
  • Susan Ha
    • 1
  • Susan K. Logan
    • 1
    Email author
  1. 1.Departments of Urology, and Biochemistry and Molecular BiologyNew York University School of MedicineNew YorkUSA
  2. 2.Institute for Systems GeneticsNew York University School of MedicineNew YorkUSA

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