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Prefoldins in Archaea

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Prefoldins: the new chaperones

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1106))

Abstract

Molecular chaperones promote the correct folding of proteins in aggregation-prone cellular environments by stabilizing nascent polypeptide chains and providing appropriate folding conditions. Prefoldins (PFDs) are molecular chaperones found in archaea and eukaryotes, generally characterized by a unique jellyfish-like hexameric structure consisting of a rigid beta-barrel backbone with protruding flexible coiled-coils. Unlike eukaryotic PFDs that mainly interact with cytoskeletal components, archaeal PFDs can stabilize a wide range of substrates; such versatility reflects PFD’s role as a key element in archaeal chaperone systems, which often lack general nascent-chain binding chaperone components such as Hsp70. While archaeal PFDs mainly exist as hexameric complexes, their structural diversity ranges from tetramers to filamentous oligomers. PFDs bind and stabilize nonnative proteins using varying numbers of coiled-coils, and subsequently transfer the substrate to a group II chaperonin (CPN) for refolding. The distinct structure and specific function of archaeal PFDs have been exploited for a broad range of applications in biotechnology; furthermore, a filament-forming variant of PFD has been used to fabricate nanoscale architectures of defined shapes, demonstrating archaeal PFDs’ potential applicability in nanotechnology.

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Acknowledgements

This work was supported by the Air Force Office of Scientific Research (FA9550-17-1-0451). S.L. was supported by a National Science Foundation Graduate Research Fellowship (DGE1106400, DGE1752814).

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, University of California, Berkeley, CA, USA

    Samuel Lim & Douglas S. Clark

  2. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia

    Dominic J. Glover

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  1. Samuel Lim
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  2. Dominic J. Glover
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  3. Douglas S. Clark
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Correspondence to Douglas S. Clark .

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Editors and Affiliations

  1. Centro Nacional de Investigaciones Oncológicas, CNIO, Madrid, Spain

    Nabil Djouder

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Lim, S., Glover, D.J., Clark, D.S. (2018). Prefoldins in Archaea. In: Djouder, N. (eds) Prefoldins: the new chaperones. Advances in Experimental Medicine and Biology, vol 1106. Springer, Cham. https://doi.org/10.1007/978-3-030-00737-9_2

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