Genetic Transformation of Pineapple

  • Ming-Li Wang
  • Robert E. PaullEmail author
Part of the Plant Genetics and Genomics: Crops and Models book series (PGG, volume 22)


Transgenic pineapple plants have been produced for the purpose of improving various economically important traits. The efforts have focused on traits such as natural flowering control, herbicide tolerance for weed control, disease and nematode control, and fruit quality traits. The fruit quality traits include increased iron and zinc levels, chilling-induced fruit browning, and increased carotenoids. Numerous constructs and approaches have been used in this normally vegetatively propagated crop. The major bottleneck for high-throughput gene functional analyses of pineapple is nucleic acid delivery and tissue culture/regeneration system. Though tissue culture is widely used commercially to increase planting material, this is slow, and the development of a plantlet following transformation culture has shown significant varietal differences and somaclonal variation. In this chapter, we focus on reviewing the technical aspects of the published transformation processes with the intention to provide practical information for planning pineapple transformation experiments.


Diseases Fruit quality Nematodes Regeneration Transformation Screening 



This research report here by the authors was partially support by the USDA NIFA Hatch Funding (HAW00862) and a Cooperative Agreement (#58-5320-3-014) between USDA ARS and HARC. The authors especially want to thank the expert professional and technical expertise of Dr. Chifumi Nagai and Ms. Gail Uruu who were involved in all the steps in the creation of transgenic pineapple in Hawaii.


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Hawaii Agriculture Research CenterKuniaUSA
  2. 2.Department of Tropical Plants and Soil Sciences, College of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluUSA

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