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Genetic Transformation of Pineapple

  • Ming-Li Wang
  • Robert E. PaullEmail author
Chapter
Part of the Plant Genetics and Genomics: Crops and Models book series (PGG, volume 22)

Abstract

Transgenic pineapple plants have been produced for the purpose of improving various economically important traits. The efforts have focused on traits such as natural flowering control, herbicide tolerance for weed control, disease and nematode control, and fruit quality traits. The fruit quality traits include increased iron and zinc levels, chilling-induced fruit browning, and increased carotenoids. Numerous constructs and approaches have been used in this normally vegetatively propagated crop. The major bottleneck for high-throughput gene functional analyses of pineapple is nucleic acid delivery and tissue culture/regeneration system. Though tissue culture is widely used commercially to increase planting material, this is slow, and the development of a plantlet following transformation culture has shown significant varietal differences and somaclonal variation. In this chapter, we focus on reviewing the technical aspects of the published transformation processes with the intention to provide practical information for planning pineapple transformation experiments.

Keywords

Diseases Fruit quality Nematodes Regeneration Transformation Screening 

Notes

Acknowledgments

This research report here by the authors was partially support by the USDA NIFA Hatch Funding (HAW00862) and a Cooperative Agreement (#58-5320-3-014) between USDA ARS and HARC. The authors especially want to thank the expert professional and technical expertise of Dr. Chifumi Nagai and Ms. Gail Uruu who were involved in all the steps in the creation of transgenic pineapple in Hawaii.

References

  1. Alan AR, Earle ED (2002) Sensitivity of bacterial and fungal pathogens to the lytic peptides, MSI-99, Magainin II and Cecropin B. Mol Plant-Microbe Interact 15:701–708CrossRefGoogle Scholar
  2. Bairu MW, Aremu AO, Van Staten J (2011) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173CrossRefGoogle Scholar
  3. Baltes NJ, Voytas DF (2015) Enabling plant synthetic biology through genome engineering. Trends Biotechnol 33:120–131CrossRefGoogle Scholar
  4. Bartholomew DP, Hawkins RA, Lopez JA (2012) Hawaii pineapple: the rise and fall of an industry. Hortscience 47:1390–1398Google Scholar
  5. Burg SP, Burg EA (1966) Auxin-induced ethylene formation: its relation to flowering in the pineapple. Science 152:1269–1269CrossRefGoogle Scholar
  6. Christensen AH, Quail PH (1996) Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res 5:213–218CrossRefGoogle Scholar
  7. Crochon M, Tisseau R, Teisson C, Huet R (1981) Effect of Ethrel aspersions before the crop on the flavouring quality of the pineapple. Fruits 36:409–415Google Scholar
  8. Daquinta R, Benegas M (1997) Brief review of pineapple tissue culture. Pineapple Newsletter 3:7–9Google Scholar
  9. Dillen W, De Clercq J, Kapila J, Zambre M, Van Motagu M, Angenon G (1997) The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants. Plant J 12:1459–1463CrossRefGoogle Scholar
  10. Dull GG, Young RE, Biale JB (1967) Respiratory patterns in fruit of pineapple, Ananas comosus, detached at different stages of development. Physiol Plant 20:1059–1065CrossRefGoogle Scholar
  11. Espinosa P, Lorenzo J, Iglesias A, Yabor L, Menéndez E, Borroto J, Hernánd L, Arencibia A (2002) Production of pineapple transgenic plants assisted by temporary immersion bioreactors. Plant Cell Rep 21:136–140CrossRefGoogle Scholar
  12. Fan C, Pu N, Wang X, Wang Y, Fang L, Xu W, Zhang J (2008) Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata. Plant Cell Tissue Organ Cult 92:197–206CrossRefGoogle Scholar
  13. Finer JJ, Vain P, Jones MW, McMullen MD (1992) Development of the particle inflow gun for DNA delivery to plant cells. Plant Cell Rep 11:323–328CrossRefGoogle Scholar
  14. Firoozabady E (2011) Organogenic transformation and regeneration. US Patent Number US8049067 B2Google Scholar
  15. Firoozabady E, Gutterson N (2003) Cost-effective in vitro propagation methods for pineapple. Plant Cell Rep 21:844–850PubMedGoogle Scholar
  16. Firoozabady E, Heckert M, Gutterson N (2006) Transformation and regeneration of pineapple. Plant Cell Tissue Organ Cult 84:1–16CrossRefGoogle Scholar
  17. Firoozabady E, Moy Y (2004) Regeneration of pineapple plants via somatic embryogenesis and organogenesis. In Vitro Cell Dev Biol Plant 40(1):67–74CrossRefGoogle Scholar
  18. Firoozabady E, Young TR (2013) Pineapple plant named Rosé (EF2-114). US Patent Number US20130326768 P1Google Scholar
  19. Fullner KJ, Nester EW (1996) Temperature affects the T-DNA transfer machineries of Agrobacterium tumefaciens. J Bacteriol 178:1498–1504CrossRefGoogle Scholar
  20. Gangopadhyay G, Bandyopadhyay T, Poddar R, Gangopadhyay SB, Mukherjee KK (2005) Encapsulation of pineapple micro shoots in alginate beads for temporary storage. Curr Sci 88:972–977Google Scholar
  21. Gangopadhyay G, Roy SK, Gangopadhyay SB, Mukherjee KK (2009) Agrobacterium-mediated genetic transformation of pineapple var. Queen using a novel encapsulation-based antibiotic selection technique. Plant Cell Tissue Organ Cult 97:295–302CrossRefGoogle Scholar
  22. Graham M, Ko L, Hardy V, Robinson S, Sawyer B, O'Hare T, Jobin M, Dahler J, Underhill S, Smith M (1999) The development of blackheart resistant pineapples through genetic engineering. Acta Hortic 529:133–138Google Scholar
  23. Green J, Nelson S (2015) Heart and root rots of pineapple. Plant Disease PD-106 published by College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Cooperative Extension ServicesGoogle Scholar
  24. Ko HL, Campbell PR, Jabin-Décor MP, Eccleston KL, Graham MW, Smith MK (2006) The introduction of transgenes to control blackheart in pineapple (Ananas comosus L.) cv. Smooth Cayenne by microprojectile bombardment. Euphytica 150:387–395CrossRefGoogle Scholar
  25. Ko L, Eccleston K, O’Hare T, Wong L, Giles J, Smith M (2013) Field evaluation of transgenic pineapple (Ananas comosus (L.) Merr.) cv. ‘Smooth Cayenne’ for resistance to blackheart under subtropical conditions. Sci Hortic 159:103–108CrossRefGoogle Scholar
  26. Ko L, Hardy V, Jabin-Décor M, Campbell P, Eccleston K, Graham M, Smith M (2005) The introduction of transgenes to control blackheart in pineapple: biolistics vs Agrobacterium transformation. In. Contributing to a Sustainable Future. International Association for Plant Tissue Culture and Biotechnology (IAPTC) Conference (Australian Branch- 7th National Meeting), 21–24 September, 2005, Perth, AustraliaGoogle Scholar
  27. Kohli A, Twyman RM, Abranches R, Wegel E, Stoger E, Christou P (2003) Transgene integration, organization, and interaction in plants. Plant Mol Biol 52:247–258CrossRefGoogle Scholar
  28. Li T, Liu B, Spalding MH, Weeks DP, Yang B (2012) High-efficiency TALEN-based gene editing produces disease-resistant rice. Nat Biotechnol 30:390–392CrossRefGoogle Scholar
  29. Lilley CJ, Urwin PE, Johnston KA, Atkinson HJ (2004) Preferential expression of a plant cystatin at nematode feeding sites confers resistance to Meloidogyne incognita and Globodera pallida. Plant Biotechnol J 2:3–12CrossRefGoogle Scholar
  30. Ma J, He Y, Wu C, Liu H, Hu Z, Sun G (2012) Effective Agrobacterium-mediated transformation of pineapple with CYC1A1 by kanamycin selection technique. Afr J Biotechnol 11:2555–2562CrossRefGoogle Scholar
  31. Mhatre M (2013) Chapter 33. Agrobacterium-mediated genetic transformation of pineapple (Ananas comosus L., Merr.). In protocols for micropropagation of selected economically-important horticultural plants. Methods Mol Biol 994:435–453CrossRefGoogle Scholar
  32. Mhatre M, Nagi L, Ganapathi TR (2009) Agrobacterium-mediated transformation of pineapple (Ananas comosus L. Merr.) leaf bases with MSI-99, a magainin analogue. Int J Fruit Sci 9:106–114CrossRefGoogle Scholar
  33. Min XJ, Bartholomew DP (1996) Effect of plant growth regulators on ethylene production, 1-aminocyclopropane-1-carboxylic acid oxidase activity, and initiation of inflorescence development of pineapple. J Plant Growth Regul 15:121–128CrossRefGoogle Scholar
  34. Ming R, VanBuren R, Wai CM, Tang H, Schatz MC, Bowers JE, Lyons E, Wang M-L, Chen J, Biggers E, Zhang J, Huang L, Zhan L, Miao W, Zhang J, Ye Z, Miao C, Lin Z, Wang H, Zhou H, Yim WC, Priest HD, Zheng C, Woodhouse M, Edger PP, Guyot R, Guo H-B, Guo H, Zheng G, Singh R, Sharma A, Min X, Zheng Y, Lee H, Gurtowski J, Sedlazeck FJ, Harkess A, McKain MR, Liao Z, Fang J, Liu J, Zhang X, Zhang Q, Hu W, Qin Y, Wang K, Chen L-Y, Shirley N, Lin Y-R, Liu L-Y, Hernandez AG, Wright CL, Bulone V, Tuskan GA, Heath K, Zee F, Moore PH, Sunkar R, Leebens-Mack JH, Mockler T, Bennetzen JL, Freeling M, Sankoff D, Paterson AH, Zhu X, Yang X, Smith JAC, Cushman JC, Paull RE, Yu Q (2015) The pineapple genome and the evolution of CAM, photosynthesis. Nat Genet 45:1435–1442CrossRefGoogle Scholar
  35. Morton ER, Fuqua C (2012) Phenotypic analyses of Agrobacterium. Curr Protoc Microbiol 3D:3Google Scholar
  36. Nagai C, Rosal L (1995) Pineapple micropropagation. Hawaii Sugar Planters’ Association Annual Report. pp 34–35Google Scholar
  37. Nan GL, Nagai C (1998) Genetic transformation of pineapple (Ananas comosus [L.] Merr.) via particle bombardment. In Vitro Cell Dev Biol Anim 34:1052Google Scholar
  38. Nan GL, Nagai C, Moore PH, Sun SSM, Sipes BS, Paull RE, Rohrbach K (1996) Tissue culture and genetic transformation studies on pineapple. In Vitro Cell Dev Biol Plant 32:102AGoogle Scholar
  39. Ni M, Cui D, Einstein J, Narasimhulu S, Vergara CE, Gelvin SB (1995) Strength and tissue specificity of chimeric promoters derived from the octopine and mannose synthase genes. Plant J 7:661–676CrossRefGoogle Scholar
  40. Norris SR, Meyer SE, Callis J (1993) The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression. Plant Mol Biol 21:895–906CrossRefGoogle Scholar
  41. Paull RE, Bartholomew DP, Chen C-C (2017) Pineapple breeding and production practices. In: Lobo MG, Paull RE (eds) Handbook of pineapple technology. Production, postharvest science, processing and nutrition. Wiley Blackwell, West Sussex, pp 16–38Google Scholar
  42. Radonic LM, Lewi DM, López NE, Hopp HE, Escandón AS, Bilbao ML (2015) Sunflower (Helianthus annuus L.). Methods Mol Biol 1224:47–55CrossRefGoogle Scholar
  43. Rose AB, Beliakoff JA (2000) Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing. Plant Physiol 122:535–542CrossRefGoogle Scholar
  44. Sander JD, Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Nat Biotechnol 32:347–355CrossRefGoogle Scholar
  45. Schünmann PHD, Llewellyn DJ, Surin B, Boevink P, De Feyter RC, Waterhouse PM (2003) A suite of novel promoters and terminators for plant biotechnology. Funct Plant Biol 30:443–452CrossRefGoogle Scholar
  46. Soneji JR, Rao MN (2009) Genetic engineering of pineapple. Transgenic Plant J 3(Special Issue 1):47–56Google Scholar
  47. Soneji JR, Rao MN, Mhatre M (2002) Somaclonal variation in micropropagated dominant axillary buds of pineapple (Ananas comosus L. Merr.). J Hortic Sci Biotechnol 77:28–32CrossRefGoogle Scholar
  48. Sripaoraya S, Marchant R, Power JB, Davey MR (2001) Herbicide-tolerant transgenic pineapple (Ananas comosus) produced by microprojectile bombardment. Ann Bot 88:597–603CrossRefGoogle Scholar
  49. Stewart RJ, Sawyer BJB, Bucheli CJ, Robinson SP (2001) Polyphenol oxidase is induced by chilling and wounding in pineapple. Aust J Plant Physiol 28:181–191Google Scholar
  50. Su G, Park S, Lee S, Murai N (2012) Low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after Agrobacterium tumefaciens-mediated transformation of tobacco leaf discs. Am J Plant Sci 3:537–545CrossRefGoogle Scholar
  51. Trusov Y, Botella JR (2006) Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]. J Exp Bot 7:3953–3960CrossRefGoogle Scholar
  52. Urnov FD, Rebar EJ, Holmes MC, Zhang HS, Gregory PD (2010) Genome editing with engineered zinc finger nucleases. Nat Rev Genet 11:636–646CrossRefGoogle Scholar
  53. Wang M-L, Uruu G, Xiong L, He X, Nagai C, Cheah KT, Hu JS, Nan G-L, Sipes BS, Atkinson HJ, Moore PH, Rohrbach KG, Paull RE (2009) Production of transgenic pineapple plants via adventitious bud regeneration. In Vitro Cell Dev Biol Plant 45:112–121CrossRefGoogle Scholar
  54. Wei H, Wang M-L, Moore PH, Albert HH (2003) Comparative expression analysis of two sugarcane polyubiquitin promoters and flanking sequences in transgenic plants. J Plant Physiol 160:1241–1251CrossRefGoogle Scholar
  55. Yabor L, Arzola M, Aragón C, Hernández M, Arencibia A, Lorenzo JC (2006) Biochemical side effects of genetic transformation of pineapple. Plant Cell Tissue Organ Cult 86:63–67CrossRefGoogle Scholar
  56. Yabor L, Espinosa P, Arencibia AD, Lorenzo JC (2006) Pineapple [Ananas comosus (L.) Merr.]. In Agrobacterium protocols, vol 2. Methods Mol Biol 344:219–226PubMedGoogle Scholar
  57. Yabor L, Valle B, Rodríguez RC, Aragón C, Papenbrock J, Tebbe CC, Lorenzo JC (2016) The third vegetative generation of a field-grown transgenic pineapple clone shows minor side effects of transformation on plant physiological parameters. Plant Cell Tissue Organ Cult 125:303–308CrossRefGoogle Scholar
  58. Zhou Y, O'Hare TJ, Jobin-Decor M, Underhill SJ, Wills RB, Graham MW (2003) Transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart. Plant Biotechnol J 1:463–478CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Hawaii Agriculture Research CenterKuniaUSA
  2. 2.Department of Tropical Plants and Soil Sciences, College of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluUSA

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