Abstract
Restoring peripheral nerve damage can be considered being an important research topic in regenerative medicine. Consequently, neuronal tissue engineering science investigates the so-called nerve conduits as promising approach to re-establish neuronal function, serving as guidance for axonal growth in vivo and possibly bridging damaged or severed nerves. In this regard, significant research has been conducted using certain biomolecules like the nerve growth factor (NGF) protein to stimulate intrinsic regenerative processes. Nevertheless, the damaged nerve tissue lacks the organotypic arrangement of cells and extracellular matrix. Here, a common approach in tissue engineering uses oriented biomaterial structures as nerve conduits with prearranged autologous neuronal cells or other cells supporting axonal growth to allow for better control of tissue-specific regeneration.
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Acknowledgements
This chapter is dedicated to our children Hanna and Emma.
The authors would like to thank the following persons for technical assistance: M. Dilger for sample preparation and staining, C. Große-Siestrup for supervision of the animal study under registration number G0445-08, F. Schmidt for STEM imaging and H. Riesemeier for supervision of the BAMline at BESSY.
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Zehbe, R., Zehbe, K. (2018). Nervous Tissue and Neuronal Cells: Patterning by Electrophoresis for Highly Resolved 3D Images in Tissue Engineering. In: Giuliani, A., Cedola, A. (eds) Advanced High-Resolution Tomography in Regenerative Medicine. Fundamental Biomedical Technologies. Springer, Cham. https://doi.org/10.1007/978-3-030-00368-5_14
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DOI: https://doi.org/10.1007/978-3-030-00368-5_14
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