Skip to main content
SpringerLink
Log in
Book cover

Progress in Anti-Cancer Chemotherapy pp 153–161Cite as

Molecular Diagnosis to Predict the Metastatic Potential of Human Prostate Cancer

  • Chapter

  • 60 Accesses

Part of the book series: Progress in Anti-Cancer Chemotherapy ((ANTI-CANCER,volume 4))

Abstract

The major cause of death from prostate cancer is due to metastases that are resistant to therapy. The prognosis and choice of therapy for most patients with prostate cancer are based on the clinical stage, serum prostate-specific antigen (PSA), and histological grade (Gleason score) of the tumor. Since metastases often develop in patients with clinically localized disease which is treated with radical prostatectomy, there is an urgent need to identify new molecular markers of prostate cancer metastasis. To produce metastasis, prostate cancer cells must complete a series of sequential and selective steps which are regulated by discrete and independent genes. We developed a rapid technique for detecting the activity of genes involved in the formation of metastasis: vascularization, invasion, adhesion, and proliferation. Human prostate cancer cells growing in culture or in the prostate of nude mice as well as formalin-fixed paraffin-embedded archival specimens of primary human prostate cancers from patients were examined by a colorimetric in situ hybridization (ISH) technique detecting mRNA of E-cadherin (cell cohesion), collagenase type IV: collagenase type IV and MMP-9 (invasion), and vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) (angiogenesis). Intra- and intertumoral heterogeneity in gene expression was prominent. The relative expression of the MMP-9 and E-cadherin genes that regulate detachment and invasion of cancer cells respectively and the expression of angiogenic factor VEGF/VPF reliably predicted the metastatic potential of individual human prostate cancers.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Landis SH, Murray T, Bolden Wingo PA (1999) Cancer statistics 1999. Cancer 49:8–31

    CAS  Google Scholar 

  2. Soh S, Kattan MW, Berkman S, Wheeler TM, Scardino PT (1997) Has there been a recent shift in the pathological features and prognosis of patients treated with radical prostatectomy? J Urol 157:2212–2218

    Article  PubMed  CAS  Google Scholar 

  3. Partin AW, Kattan MW, Subond ENP, Walsh PC, Wojno KT, Oesterline JE et al (1997) The use of prostate-specific antigen, clinical stage, and Gleason score to predict pathological stage I men with localized prostate cancer: a multi-institutional update. J Am Med Assoc 277:1445–1451

    Article  CAS  Google Scholar 

  4. Gleason DF, Mellinger GT, The Veterans Administrative Cooperative Urologie Research Group (1998) Histologic grading and clinical staging of prostatic carcinoma. In: Tannenbaum M. (ed) Urologic pathology: the prostate. Lea & Febiger, Philadelphia, p 171

    Google Scholar 

  5. Zincke H, Oesterling JE, Blute ML, Bergstralh EJ, Myers RP, Barrett DM (1994) Long-term (15 years) results after radical prostatectomy for clinically localized (stage T2c or lower) prostate cancer. J Urol 152:1850–1857

    PubMed  CAS  Google Scholar 

  6. Kitadai Y, Ellis LM, Takahashi Y, Bucana CD, Anzai H, Tahara E, Fidler IJ (1995) Multiparametric in situ messenger RNA hybridization analysis to detect metastasis-related genes in surgical specimens of human colon carcinomas. Clin Cancer Res 1:1095–1102

    PubMed  CAS  Google Scholar 

  7. Fidler IJ (1990) Critical factors in the biology of human cancer metastasis: twenty-eighth GHA Clowes Memorial Award Lecture. Cancer Res 50:6130–6138

    PubMed  CAS  Google Scholar 

  8. Fidler IJ (1995) Modulation of the organ microenvironment for the treatment of cancer metastasis (commentary). J Natl Cancer Inst 87:1588–1592

    Article  PubMed  CAS  Google Scholar 

  9. Radinsky R (1993) Paracrine growth regulation of human colon carcinoma organ-specific metastasis. Cancer Metastasis Rev 12:345–361

    Article  PubMed  CAS  Google Scholar 

  10. Mendelsohn J (1990) The epidermal growth factor receptor as a target for therapy with antireceptor monoclonal antibodies. Semin Cancer Biol 1:339–344

    PubMed  CAS  Google Scholar 

  11. Radinsky R, Risin S, Fan D, Dong Z, Bielenberg DR, Bucana CD, Fidler IJ (1995) Level and function of epidermal growth factor receptor predict the metastatic potential of human colon carcinoma cells. Clin Cancer Res 1:19–31

    PubMed  CAS  Google Scholar 

  12. Folkman MJ (1992) The role of angiogenesis in tumor growth. Semin Cancer Biol 3:65–71

    PubMed  CAS  Google Scholar 

  13. Liotta LA, Stetler-Stevenson WG (1985) Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell 64:317–326

    Google Scholar 

  14. Singh RK, Gutman M, Bucana CD, Sanchez R, Llansa N, Fidler IJ (1995) Interferons-α and -β downregulate the expression of basic fibroblast growth factor in human carcinomas. Proc Natl Acad Sci USA 92:4562–4566

    Article  PubMed  CAS  Google Scholar 

  15. Singh RK, Gutman M, Radinsky R, Bucana CD, Fidler IJ (1994) Expression of interleukin-8 correlates with the metastatic potential of human melanoma cells in nude mice. Cancer Res 54:3242–3247

    PubMed  CAS  Google Scholar 

  16. Nakajima M, Morikawa K, Fabra A, Bucana CD, Fidler IJ (1990) Influence of the organ environment on extracellular matrix degradative activity and metastasis of human colon carcinoma cells. J Natl Cancer Inst 82:1890–1898

    Article  PubMed  CAS  Google Scholar 

  17. Boag AH, Young ID (1994) Increased expression of 72-kd type IV collagenase in prostatic adenocarcinoma. Am J Pathol 144:585–591

    PubMed  CAS  Google Scholar 

  18. Weinstein RS, Jakate SM, Dominguez JM, Lebovitz MD, Koukoulis GK, Kuszak JR, Dlusens LF, Grogan TM, Saclarides TJ, Roninson IB, Coon JS (1991) Relationship of the expression of the multidrug resistance gene product (P-glycoprotein) in human colon carcinoma to local tumor aggressiveness and lymph node metastasis. Cancer Res 51:2720–2726

    PubMed  CAS  Google Scholar 

  19. Shimoyama Y, Hirohashi S, Hirano S, Noguchi M, Shimasato Y, Takeichi M, Abe О (1989) Cadherin cell adhesion molecules in human epithelial tissues and carcinomas. Cancer Res 49:2128–2133

    PubMed  CAS  Google Scholar 

  20. Weinstein RS, Jakate SM Dominguez JM, Lebovitz MD, Koukoulis GK, Kuzak JR et al. (1991) Relationship of the expression of the multidrug resistance gene product (P-glycoprotein) in human colon carcinoma to local tumor aggressiveness and lymph node metastasis. Cancer Res 1:2720–2726

    Google Scholar 

  21. Theyer G, Schirmbock M, Thalhammer T, Sherwood ER, Baumgartner G, Hamilton G (1993) Role of the MDR-1 encoded multiple drug resistance phenotype in prostate cancer cell lines. J Urol 150:1544–1547

    PubMed  CAS  Google Scholar 

  22. Takeichi M. (1990) Cadherins: a molecular family important in selective cell-cell adhesion. Ann Rev Biochem 59:237–252

    Article  PubMed  CAS  Google Scholar 

  23. Mayer B, Johnson JP, Leitl F, Jauch KW, Heiss MM, Schildberg FW et al. (1993) E-cadherin expression in primary and metastatic gastric cancer: downregulation correlates with cellular dedifferentiation and glandular disintegration. Cancer Res 53:1690–1695

    PubMed  CAS  Google Scholar 

  24. Fidler IJ, Radinsky R (1990) Genetic control of cancer metastasis (editorial). J Natl Cancer Inst 82:166–168

    Article  PubMed  CAS  Google Scholar 

  25. Bucana CD, Radinsky R, Dong Z, Sanchez R, Brigati DJ, Fidler IJ (1993) A rapid colorimetric in situ mRNA hybridization technique using hyperbiotinylated oligonucleotide probes for analysis of mdr-1 in mouse colon carcinoma cells. J Histochem Cytochem 41:499–506

    Article  PubMed  CAS  Google Scholar 

  26. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448

    Article  PubMed  CAS  Google Scholar 

  27. Greene G, Kitadai Y, Pettaway CA, von Eschenbach AC, Fidler IJ (1997) Correlation of metastasis-related gene expression with metastatic potential in prostate carcinoma cells implanted in nude mice using an in situ mRNA hybridization technique. Am J Pathol 150:1571–1582

    PubMed  CAS  Google Scholar 

  28. Reed JA, Manahan LJ, Park CS, Brigati DJ (1992) Complete one-hour immunocytoche-mistry based on capillary action. Biotechniques 13:434–443

    PubMed  CAS  Google Scholar 

  29. Balbay MD, Pettaway CA, Kuniyasu H, Inoue K, Ramirez E, Li E et al. (1999) Highly metastatic human prostate cancer growing within the prostate of athymic mice overexpresses a vascular endothelial growth factor. Clin Cancer Res 5:783–789

    PubMed  CAS  Google Scholar 

  30. Kitadai Y, Ellis LM, Tucker SL, Greene GF, Bucana CD, Cleary KR, Takahashi Y, Tahara E, Fidler, IJ (1996) Multiparametric in situ mRNA hybridization analysis to predict disease recurrence in patients with colon carcinoma. Am J Pathol 149:1541–1551

    PubMed  CAS  Google Scholar 

  31. Anzai H, Kitadai Y, Bucana CD, Sanchez R, Omoto R, Fidler IJ (1996) Intratumoral heterogeneity and inverse correlation between expression of E-cadherin and collagenase type IV in human gastric carcinomas. Differentiation 60:119–127

    Article  PubMed  CAS  Google Scholar 

  32. Kuniyasu H, Ellis LM, Evans DB, Abbruzzese JL, Fenoglio CJ, Bucana CD, Cleary KR, Tahara E, Fidler IJ (1999) Relative expression of E-cadherin and type IV collagenase genes predicts disease outcome in patients with resectable pancreatic carcinoma. Clin Cancer Res 5:25–33

    PubMed  CAS  Google Scholar 

  33. Bastacky SI, Walsh PC, Epstein JI (1993) Relationship between perineural invasion on needle biopsy and radical prostatectomy capsular penetration in clinical stage В adenocarcinoma of the prostate. Am J Surg Pathol 17:336–341

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. I. J. Fidler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Kuniyasu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. F. Greene
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Troncoso
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. A. Pettaway
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Pitié-Salpétrière Hospital SOMPS, 47, bd de l’Hôpital, 75651, Paris Cedex 13, France

    David Khayat

  2. MD Anderson Cancer Center, Department of Breast Medical Oncology, University of Texas, 1515 Holcombe Boulevard, 77030, Houston, Texas, USA

    Gabriel N. Hortobagyi

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer-Verlag France

About this chapter

Cite this chapter

Fidler, I.J., Kuniyasu, H., Greene, G.F., Troncoso, P., Pettaway, C.A. (2000). Molecular Diagnosis to Predict the Metastatic Potential of Human Prostate Cancer. In: Khayat, D., Hortobagyi, G.N. (eds) Progress in Anti-Cancer Chemotherapy. Progress in Anti-Cancer Chemotherapy, vol 4. Springer, Paris. https://doi.org/10.1007/978-2-8178-0920-5_10

Download citation

  • DOI: https://doi.org/10.1007/978-2-8178-0920-5_10

  • Publisher Name: Springer, Paris

  • Print ISBN: 978-2-287-59692-6

  • Online ISBN: 978-2-8178-0920-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation