Skip to main content
SpringerLink
Log in

Mobilization, purification and ex vivo expansion of human peripheral blood progenitor cells

  • Conference paper
Book cover Cancer Treatment An Update

  • 71 Accesses

Abstract

Peripheral blood progenitor cells (PBPCs) have been used with increasing frequency for autografting following high-dose chemotherapy [1]. Successful transplantation of PBPCs with complete and sustained engraftment has been shown in a variety of disorders, including ANLL, lymphoma, neuroblastoma, breast cancer, and other solid tumors [1–8]. When compared with autologous bone marrow transplantation, the advantages of this modality include a more rapid restoration of neutrophils and platelets, the possibility of autografting when bone marrow aspiration is hampered by tumor cell infiltration, fibrosis or hypoplasia following radio-/chemotherapy, and the possibility of reduced contamination with malignant cells in disseminated cancer [9]. The actual level of malignant cell contamination, however, is a matter of debate [10] as well as the exact number of mononuclear cells, CFU-GM colonies or CD34+ cells which have to be transplanted in order to allow a complete and sustained hematopoietic reconstitution [11, 12].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Kessinger A, Armitage JO (1991) The evolving role of autologous peripheral stem cell transplantation following high-dose chemotherapy for malignancies. Blood 77: 211–213

    PubMed  CAS  Google Scholar 

  2. Körbling M, Holle R, Haas R, Knauf W, Dörken B, Ho AD, Kuse R, Pralle H, Fliedner TM, Hunstein W (1990) Autologous blood stem-cell transplantation in patients with advanced Hodgkin’s disease and prior radiation to the pelvic site. J Clin Oncol 8: 978–985

    PubMed  Google Scholar 

  3. Kessinger A, Armitage JO, Smith DM, Landmark JD, Bierman PJ, Weisenburger DD (1989) High-dose therapy and autologous peripheral blood stem cell transplantation for patients with lymphoma. Blood 74: 1260–1266

    PubMed  CAS  Google Scholar 

  4. Körbling M, Dörken B, Ho AD et al (1986) Autologous transplantation of blood-derived hematopoietic stem cells after myeloablative therapy in a patient with Bur-kitt’s lymphoma. Blood 72: 529–532

    Google Scholar 

  5. Juttner CA, To LB, Ho JQK et al (1988) Early lympho-hematopoietic recovery after autografting using peripheral blood stem cells in acute non-lymphoblastic leukemia. Transplant Proc 20: 40

    PubMed  CAS  Google Scholar 

  6. Sheridan WP, Begley CG, Juttner C, Szer J, To LB, Maher D, McGrath KM, Morstyn G, Fox RM (1992) Effect of peripheral-blood progenitor cells mobilised by filgrastim (G-CSF) on platelet recovery after high-dose chemotherapy. Lancet i: 640

    Article  Google Scholar 

  7. Siena S, Bregni M, Brando B, Belli N, Ravagnani F, Gondola L, Stern AC, Landsdorp PM, Bonadonna G, Gianni AM (1991) Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. Blood 77: 400–406

    PubMed  CAS  Google Scholar 

  8. Elias AD, Ayash L, Anderson C, Hund M, Wheller C, Schwartz G, Telpler I, Mazanet R, Lynch C, Pap S, Pelaez J, Reich E, Critchlow J, Demetri G, Bibbo J, Schnipper L, Griffin JD, Frei E, Antman H (1992) Mobilization of peripheral blood progenitor cells by chemotherapy and granulocyte-macrophage colony-stimulating factor for hematologic support after high-dose intensification in breast cancer. Blood 79: 3036–3042

    PubMed  CAS  Google Scholar 

  9. To LB, Russel J, Moore S, Juttner CA (1987) Residual leukemia cannot be detected in very early remission peripheral blood stem cell collections in acute non-lymphoblastic leukemia. Leuk Res 11: 327

    Article  PubMed  CAS  Google Scholar 

  10. Moss TJ, Sandes DG, Lasky LC, Bostrom B (1990) Contamination of peripheral blood stem cell harvests by circulating neuroblastoma cells. Blood 75: 1879–1883

    Google Scholar 

  11. Lasky LC, Ash RC, Kersey JH, Zanjani ED, McCullough J (1982) Collection of pluripotential hematopoietic stem cells by cytapheresis. Blood 59: 822

    PubMed  CAS  Google Scholar 

  12. Juttner CA, To LB, Haylock DN, Cyson PG, Thorp D, Dart GW, Ho JQK, Horvath N, Bardy P (1989) Autologous blood stem cell transplantation. Transplant Proc 21: 2929

    PubMed  CAS  Google Scholar 

  13. Richman CM, Weiner RS, Yankee RA (1976) Increase in circulating stem cells following chemotherapy in man. Blood 47: 1031

    PubMed  CAS  Google Scholar 

  14. Abrams RA, McCormack, Bowles C, Deisseroth AB (1981) Cyclophosphamide treatment expands the circulating haemopoietic stem cell pool in dogs. J Clin Invest 67: 1392

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  15. To LB, Shepperd M, Haylock DN, Dyson PG, Charles P, Thorp L, Dale BM, Dart GW, Roberts MM, Sage RE (1989) Single high doses of cyclophosphamide enable the collection of high numbers of haemopoietic stem cells from the peripheral blood. Exp Hematol 18: 442

    Google Scholar 

  16. Haas R, Ho AD, Bredthauer U, Cayeux S, Egerer G, Knauf W, Hunstein W (1990) Successful autologous transplantation of blood stem cells mobilized with rhGM-CSF. Exp Hematol 18: 94

    PubMed  CAS  Google Scholar 

  17. Socinski MA, Cannistra SA, Elias A, Antman KH, Schnipper L, Griffin JD (1988) GM-CSF expands the circulating haemopoietic progenitor cell compartment in man. Lancet ii: 1194–1200

    Article  Google Scholar 

  18. Villeval JL, Dührsen U, Morstyn G, Metcalf D (1990) Effect of recombinant human GM-CSF on progenitor cells in patients with advanced malignancies. Br J Hematol 74: 36

    Article  CAS  Google Scholar 

  19. Dührsen U, Villeval JL, Boyd J, Kannourakis G, Morstyn G, Metcalf D (1988) Effects of recombinant human G-CSF on hematopoietic progenitor cells in cancer patients. Blood 72: 2074

    PubMed  Google Scholar 

  20. Pettengell R, Demuynck H, Testa NG, Dexter TM (1992) The engraftment capacity of peripheral blood progenitor cells mobilized with chemotherapy ± G-CSF. Int J Cell Clon 10: 59–61

    Article  Google Scholar 

  21. Gianni AM, Siena S, Bregni M, Tarella C, Stern AC, Pileri A, Bonadonna G (1989) Granulocyte-macrophage colony-stimulating factor to harvest circulating haemopoietic stem cells for autotransplantation. Lancet ii: 580–585

    Article  Google Scholar 

  22. Kanz L, Brugger W, Bross KJ, Frisch J, Schulz G, Mertelsmann R (1991) IL-3+ GM-CSF induced mobilization of myeloid, erythroid, multilineage as well as megakaryocytic progenitor cells following polychemotherapy in cancer patients. Blood 78: 1222 (abstr)

    Google Scholar 

  23. Brugger W, Bross J, Frisch J, Dern P, Weber B, Mertelsmann R, Kanz L (1992) Mobilization of PBPCs by sequential administration of IL-3 and GM-CSF following poly-chemotherapy with etoposide, Ifosfamide and Cisplatin. Blood 79: 1193–1200

    PubMed  CAS  Google Scholar 

  24. Kanz L, Brugger W, Bross J, Mertelsmann R (1992) Correlation analyses between CD34+ cells and clonogenic progenitors mobilized into the peripheral blood by IL-3+ GM-CSF following polychemotherapy in cancer patients. Int J Cell Cloning 10: 68–70

    Article  Google Scholar 

  25. Brugger W, Frisch J, Schulz G, Mertelsmann R, Kanz L (1992) Sequential administration of IL-3 and GM-CSF following standard-dose combination chemotherapy with Etoposide, Ifosfamide and Cisplatin. J Clin Oncol 10: 1193–1200

    Google Scholar 

  26. Brugger W, Birken R, Bertz H, Hecht T, Pressler, Frisch J, Schulz G, Mertelsmann R, Kanz L (1993) Peripheral blood progenitor cells mobilized by chemotherapy plus G-CSF accelerate both neutrophil and platelet recovery after high-dose VP16, Ifosfamide, and Cisplatin. Br J Hematol (in press)

    Google Scholar 

  27. Brugger W, Möcklin W, Heimfeld S, Berenson RJ, Mertelsmann R, Kanz L (1993) Ex vivo expansion of enriched peripheral blood CD34+ progenitor cells by stem cell factor, interleukin-1/3, lnterleukin-6, Interleukin-3, Interferon-gamma, and Erythropoietin. Blood (in press)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hematology and Oncology, Albert-Ludwigs University Medical Center, 7800, Freiburg, Germany

    W. Brugger, R. Mertelsmann & L. Kanz

Authors
  1. W. Brugger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Mertelsmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Kanz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Hôpital Saint-Louis, 1, avenue Vellefaux, 75475, Paris Cedex 10, France

    P. Banzet

  2. The Mount Sinai Medical Center, 1 G. Levy Place, New York, NY, 10029-6574, USA

    J. F. Holland

  3. Service d’Oncologie Médicale, Hôpital Pitié-Salpétrière Pavillon Jacquart, 47, boulevard de l’Hôpital, 75651, Paris Cedex 13, France

    D. Khayat

  4. Hôpital Pitié-Salpétrière Pavillon Jacquart, 47, boulevard de l’Hôpital, 75651, Paris Cedex 13, France

    M. Weil

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer-Verlag France

About this paper

Cite this paper

Brugger, W., Mertelsmann, R., Kanz, L. (1994). Mobilization, purification and ex vivo expansion of human peripheral blood progenitor cells. In: Banzet, P., Holland, J.F., Khayat, D., Weil, M. (eds) Cancer Treatment An Update. Springer, Paris. https://doi.org/10.1007/978-2-8178-0765-2_6

Download citation

  • DOI: https://doi.org/10.1007/978-2-8178-0765-2_6

  • Publisher Name: Springer, Paris

  • Print ISBN: 978-2-8178-0767-6

  • Online ISBN: 978-2-8178-0765-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation