Abstract
This chapter covers the use of a stereotaxic atlas and cranial landmarks to determine the spatial coordinates of a brain structure and the handling of a stereotaxic apparatus. From a short definition of stereotaxy, the reader, already familiar with the three-dimensional reference system described in Chap. 2, is first guided through a presentation of the main atlases available for rats and mice and a detailed description of a conventional stereotaxic apparatus. A short section notably addresses the issue of stereotaxic precision and describes the principle of a vernier and its use for the measurement of the distances and positions of stereotaxic holders and micropositioners. The main part of this chapter then covers the methodology for a correct determination of the anteroposterior, mediolateral, and dorso-ventral coordinates of a brain structure from the cranial landmarks bregma and lambda. Importantly, this section also addresses the topographical organization of blood vessels that may prevent or constrain the insertion of foreign material such as cannulae at a given set of coordinates. Examples are given with two brain structures, the substantia nigra pars reticulata (SNr), approached vertically, and the vertical limb of the diagonal band of Broca, approached with a 10° mediolateral angle. Illustrated with figures this chapter is aimed to help users in performing accurate determination and measurements of stereotaxic coordinates.
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References
Albe-Fessard D, Stutinsky F, Libouan S (1966) Atlas stéréotaxique du diencéphale du rat blanc. Éditions du Centre National de la Recherche Scientifique, Paris
Altman J, Bayer SA (1994) Atlas of prenatal rat brain development. CRC Press, Inc., Boca Raton
Cooley RK (1990) Stereotaxic surgery in the rat: a photographic series. A.J. Kirby Co, London
Dashwood MR, Loesch A (2010) Endothelin-1 as a neuropeptide: neurotransmitter or vascular effects? J Cell Commun Signal 4(1):51–62
De La Porte C (1993) Technical possibilities and limitations of stereotaxy. Acta Neurochir (Wien) 124(1):3–6
Franklin KBJ, Paxinos G (2008) The mouse brain in stereotaxic coordinates, 3rd edn. Academic/Elsevier, Oxford
König JFR, Klippel RA (1963) The rat brain: a stereotaxic atlas of the forebrain and the lower parts of the brain stem. Williams and Wilkins, Baltimore
Lehmann A, Gautier M, Ghilini G, Henry J, Langlois R, Laplante S (1974) Atlas stéréotaxique du cerveau de la souris. Éditions du Centre de la Recherche Scientifique, Paris
Montemurro DG, Dukelow RH (1971) A stereotaxic atlas of the diencephalon and related structures of the mouse. Mount Kisco/Future, New York
Paxinos G, Franklin KBJ (2001) The mouse brain in stereotaxic coordinates, 2nd edn. Elsevier, New York
Paxinos G, Franklin KBJ (2003) The mouse brain in stereotaxic coordinates: compact, Compact 2nd edn. Elsevier, New York
Paxinos G, Franklin KBJ (2012) The mouse brain in stereotaxic coordinates, Compact 4th edn. Elsevier, New York
Paxinos G, Watson C (1998) The rat brain in stereotaxic coordinates, Compact 4th edn. Academic, New York
Paxinos G, Watson C (2004) The rat brain in stereotaxic coordinates, 5th edn. Academic, San Diego
Paxinos G, Watson C (2007) The rat brain in stereotaxic coordinates, 6th edn. Academic, San Diego
Paxinos G, Watson C (2013) The rat brain in stereotaxic coordinates, 7th edn. Academic, San Diego. ISBN 9780123919496
Pellegrino LJ, Cushman AJ (1967) A stereotaxic atlas of the rat brain. Appleton-Century-Crofts, New York
Schambra UB, Lauder JM, Silver J (1991) Atlas of the prenatal mouse brain. Academic, New York
Sidman RL, Angevine JB Jr, Taber PE (1971) Atlas of the mouse brain and spinal cord. Harvard University Press, Cambridge. Mass
Swanson L (2003) Brain maps: structure of the rat brain, 3rd edn. Elsevier, New York
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Ferry, B., Gervasoni, D., Vogt, C. (2014). Stereotaxic Approach of a Target Structure. In: Stereotaxic Neurosurgery in Laboratory Rodent. Springer, Paris. https://doi.org/10.1007/978-2-8178-0472-9_4
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DOI: https://doi.org/10.1007/978-2-8178-0472-9_4
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