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Cord Blood Stem Cell Expansion Ex Vivo: Current Status and Future Strategies

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Regenerative Medicine Using Pregnancy-Specific Biological Substances

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Abstract

Reconstitution of immune system with allogeneic hematopoietic stem cells (HSCs) appears to be critical for the cure of hematopoietic malignancies and advanced autoimmune diseases. Cord blood (CB) HSCs is an ideal resource for the reconstitution. However, the number of HSCs in each CB unit is not sufficient for the patients who require multiple HSC transplantations. Large ex vivo expansion of transplantable CB HSCs may overcome the difficulty. Ideally, the expanded CB HSCs should be safe without the risk of cell transformation, preserve the capability of self-renewal and multipotency of differentiation, and be competent in long-term repopulation. Although we are far away from the criteria, recently, ex vivo expansion of CB HSCs has been extensively investigated in line with the progress in stem cell biology, and would bring a huge hope for cure of diseases by transplantation of CB HSCs. In this chapter, we will review the recent progress in ex vivo expansion of CB HSCs as well as current understandings of HSCs, including cellular and molecular bases useful for ex vivo expansion of HSCs.

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Authors and Affiliations

  1. Department of Pathology and Comprehensive Cancer Center, Ohio State University, Columbus, OH, 43210, USA

    Jian-Xin Gao

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  1. Jian-Xin Gao
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  2. Quansheng Zhou
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Correspondence to Jian-Xin Gao .

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  1. Advanced Medical Research Inst., B.P.Poddar Hospital, Vidyasagar Hospital, Kolkata, India

    Niranjan Bhattacharya

  2. Dept. Obstetrics & Gynaecology, Boston University Medical Centre, E. Concord Street 85, Boston, 02118, Massachusetts, USA

    Phillip Stubblefield

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Gao, JX., Zhou, Q. (2011). Cord Blood Stem Cell Expansion Ex Vivo: Current Status and Future Strategies. In: Bhattacharya, N., Stubblefield, P. (eds) Regenerative Medicine Using Pregnancy-Specific Biological Substances. Springer, London. https://doi.org/10.1007/978-1-84882-718-9_26

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