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Advances in Stem Cell Research pp 29–48Cite as

Biomanufacturing Human Pluripotent Stem Cells for Therapeutic Applications

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) can be propagated indefinitely and have the potential to differentiate to all cell types of the body. In addition, patient-specific human iPSCs and hESCs containing point mutations in genes that cause disease can be used to study diseases that have no adequate human in vitro or animal models. Harnessing the potential of these cells holds promise for future applications in cell therapy and regenerative medicine. Common methods of expanding and differentiating human pluripotent stem cells (hPSCs), including hESC and iPSC, require serum, mouse or human feeder cells, or feeder-conditioned medium. These methods are labor intensive and hard to scale, and sources of variability including growth factor fluctuations during preparation and culture complicate large-scale hPSC bioprocesses. Biomanufacturing cells from hPSCs requires development of fully defined, xeno-free culture medium and substrates formulated with human-derived, human recombinant proteins or chemically synthetic substrates under cGMP and improved processes for monitoring cell status and genomic stability during expansion and differentiation. In addition, robust and scalable differentiation methods must be developed. This chapter discusses recent progress and remaining challenges facing production of hPSC-derived cells.

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Acknowledgments

The authors thank members of the Palecek lab for helpful discussions of the topic matter. Support for this chapter was provided by the National Science Foundation grant EFRI-0735903 and National Institute of Biomedical Imaging and Bioengineering grant R01EB007534.

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  1. Department of Chemical and Biological Engineering, University of Wisconsin, Madison, WI, USA

    Xiaojun Lian & Sean P. Palecek

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  1. Xiaojun Lian
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Correspondence to Sean P. Palecek .

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  1. Royan Institute, Department of Developmental Biology, University of Science and Culture, PO Box 19395-4644, Tehran, Iran

    Hossein Baharvand

  2. , Regenerative Medicine, Royan Institute for Stem Cell Biology an, P.O. Box: 19395-4644, Tehran, Iran

    Nasser Aghdami

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Lian, X., Palecek, S.P. (2012). Biomanufacturing Human Pluripotent Stem Cells for Therapeutic Applications. In: Baharvand, H., Aghdami, N. (eds) Advances in Stem Cell Research. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-940-2_3

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