Abstract
Emerging technology has the potential to provide solutions to the devastating complications of illnesses, for people of all ages and genders and all backgrounds. Nevertheless, there are difficulties. Perhaps the most challenging area is transplantation, and in particular using stem cells. Transplantation implies contact, hence surface interactions, between the stem cell and the host tissue. Attachment and spreading of a cell on a substratum are the first part of the process that leads to the ultimate assimilation of the new cell into the host tissue. Together with confocal microscopy, we have exploited a uniquely powerful non-invasive optical technique and a 3-D microfluidic system by integrating a hydrogel scaffold into a PDMS device for cell growth, with co-culture capability to quantity attachment and spreading, and determine how the cell environment (the substratum, which might be tissue or an artificial non-living implant (prosthesis); the complex liquid medium bathing the cell; and the possible presence of congeners) influence attachment and spreading. This novel microfluidic platform has proven to be a versatile and powerful tool to study cell migration for various biological applications. This chapter highlights an overview of the application of Micro/Nanotechnology to stem cell research and technology.
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Notes
- 1.
The aim of the study was to examine of the potential of ESCs to differentiate into dopamine neurons and integration within the host brain.
- 2.
Iron oxide nanoparticles are used of a wide variety of physical, chemical and biological processes such as diagnostic medicine.
References
Moore MT, Levine SR (2006) People help drive progress. In: Lanza R (ed) Essentials of stem cell biology. Elsevier, Burlington, p 513
Poole CP, Owens FJ (2003) Introduction to nanotechnology. Wiley, New York
Ozin GA, Arsenault AC (2005) Nanochemistry. RSC Publishing, Cambridge
Ramsden JJ (2005) What is nanotechnology? Nanotechnol Precept 1:3–17
Feynman RP (1959) There’s plenty of room at the bottom. http://www.zyvex.com/nanotech/feynman.html. Accessed 10th Sept 2007
Binnih B, Rohrer H (1982) Scanning tunneling microscopy. Helv Phys Acta 55:38–47
Niemeyer C (2004) Nanobiotechnology: concepts, applications and perspectives. Wiley-VCH, Weinheim
Baharvand H, Zare N (2008) Nanotechnology application in stem cell biology and technology. In: Reisner D, Bronzino J (eds) Bionanotechnology. CRC Press, Boca Raton, p 1–43
Khademhosseini A, Langer R, Borenstein J, Vacanti J (2006) Microscale technologies for tissue engineering and biology. Proc Natl Acad Sci U S A 103:2480–2487
Khademhosseini A (2008) Micro- and nanoengineering of the cell microenvironment: technologies and applications. Artech House, Norwood
Lanza R, Gearhart J, Hogan B, Melton D, Pedersen R (2006) Essentials of stem cell biology. Academic Press, New York
Zamir E, Geiger B (2001) Components of cell-matrix adhesions. J Cell Sci 114:3577–3579
Ramsden JJ (2008) Biomedical Surfaces, 1st edn. Artech House, Norwood
Pritinder K, Li A (2000) Adhesive properties of human basal epidermal cells: an analysis of keratinocyte stem cells, transit amplifying cells and postmitotic differentiating cells. J Invest Dermatol 114:413–420
McColl J, Horvath R, Aref A, Larcombe L, Morgan S, Chianella I, Yakubov G, Ramsden J (2009) Polyphenol control of cell spreading on glycoprotein substrates. J Biomater Sci Polym Ed 20:841–851
Pennisi E (1998) How a growth control pathway takes a wrong turn to cancer. Science 281:1439–1441
Dinesh S (2006) Stem cell attachment to layer-by-layer assembled TiO2 nanoparticle thin films. Biomaterials 27:4294–4303
Griffith LG (2002) Emerging design principles in biomaterials and scaffolds for tissue engineering. Ann N Y Acad Sci 961:83–95
Bissell MJ, Barcellos-Hoff MH (1987) The influence of extracellular matrix on gene expression: is structure the message? J Cell Sci Suppl 8:327–343
Smith LA, Ma PX (2004) Nano-fibrous scaffolds for tissue engineering. Coll Surf B 39:125–131
Dalby MJ, Riehle M, Sutherland S, Agheli H, Curtis A (2004) Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography. Biomaterials 25:5415–5422
Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920–926
Zuwei Ma, Masaya T (2005) Potential of nanofiber matrix as tissue engineering scaffolds. Tissue Eng 11:101–109
Cukierman E (2001) Taking cell-matrix adhesions to the third dimension. Science 294:1708–1712
Nur EKA (2005) Three dimensional nanofibrillar surfaces induce activation of Rac. Biochem Biophys Res Commun 331:428–434
Senesi GS, D’Aloia E , Gristina R, Favia P, d’Agostino R (2007) Surface characterization of plasma deposited nano-structured fluorocarbon coatings for promoting in vitro cell growth. Surf sci 601:1019–1025
Meng J, Li S, Jie M, Hua K, Guangjin Z, Wang C, Xu L, Xie S, Xu H (2006) Using single-walled carbon nanotubes nonwoven films as scaffolds to enhance long-term cell proliferation in vitro. J Biomed Mater Res Part A 79A:298–306
Chavany C, Behmoaras T, Puisieux F, Helene C (1994) Adsorption of oligonucleotides onto polyisohexycya nanocryslate nanoparticles protects them against nucleases and increases their cellular uptake. Pharm Res 11:1370–1378
Janes K, Calvo P, Alonso MJ (2001) Polysacharaide colloidal particles as delivery systems for macromolecules. Adv Drug Deliv Rev 47:83–97
Braydich-Stolle L, Hussain S, Schlager J, Hofmann M (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci 88:412–419
Flemming RG, Murphy CJ, Abrams GA, Goodman SL, Nealey P (1998) Effect of synthetic micro-and nano-structured surfaces on cell behaviour. Biomaterials 20:573–588
Rosenthal S, Tolinson I, Adkins E (2001) Targeting cell surface receptor with ligand-conjugated nanocrystals. J Am Chem Soc 124:4586–4594
Paradise M, Goswami T (2007) Carbon nanotubes—Production and industrial applications. Mater Design 28:1477–1489
Chen J, Hamon MA, Hu H, Chen Y, Rao A, Eklund P, Haddon PC (1998) Solution properties of single-walled carbon nanotubes. Sceince 282:95–98
Mattson M, Haddon RC, Rao AM (2000) Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth. J Mol Neurosci 123:3838
Hui H, Ni Y, Montana V, Haddon R, Parpura V (2004) Chemically functionalized carbon nanotubes as substate for neuronal growth. Nano Lett 4:507–511
Lovat V, Pantarotto D, Lagostena L (2005) Carbon nanotube substrates boost neuronal electrical signalling. Nano Lett 5:1107–1110
Li WJ (2002) Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J Biomed Mater Res 60:613–621
Li WJ (2003) Biological response of chondrocytes cultured in three-dimensional nanofibrous poly (epsilon-caprolactone) scaffolds. J Biomed Mater Res 67A:1105–1114
Ansari F, Horvath R, Aref A, Ramsden JJ (2008) Bacterial adsorption onto a thin Fe3O4 magnetic nanofilms. In: 11th annual nanotechnology conference and trade show, Boston, MA, U S A, June 1–5, p 66
Berry CC, Curtis A (2003) Functionalisation of magnetic nanoparticles for applications in biomedicine. J Phys D Appl Phys 36:198–206
Riviere C (2005) Iron oxide nanoparticle-labeled rat smooth muscle cells: cardiac MR imaging for cell graft monitoring and quantitation. Radiology 235:959–967
Shapiro EM (2006) In vivo detection of single cells by MRI. Magn Reson Med 55:242–249
Hoehn M (2002) Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proc Natl Acad Sci U S A 99:16267–16272
Payne AG (2004) Using immunomagnetic technology and other means to facilitate stem cell homing. Med Hypotheses 62:718–720
Bjorklund LM (2002) Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model. Proc Natl Acad Sci 99:2344–2349
Bulte J, Kraitchman D (2004) Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed 17:484–499
Guo P (2005) RNA nanotechnology: engineering, assembly and applications in detection, gene delivery and therapy. J Nanosci Nanotechnol 5:1964–1982
Vo-Dinh T (2006) Nanoprobes and nanobiosensors for monitoring and imaging individual living cells. Nanomed Nanotechnol Biol Med 2:22–30
Ramsden JJ (1995) Experimental methods for investigating protein adsorption kinetics at surfaces. Q Rev Biophys 27:41–105
Gryte DM, Ward MD, Hu W-S (1993) Real-time measurement of anchorage-dependent cell adhesion using a quartz crystal microbalance. Biotechnol Prog 9:105–108
Fredrikkson C, Kihlmann S, Rodahl M, Kasemo B (1998) The piezoelectric quartz crystal mass and dissipation sensor: a means of studying cell adhesion. Langmuir 14:248–251
Nimeri G, Fredrikkson C, Elwing H, Liu L, Rodahl M, Kasemo B (1998) Neutrophil interaction with protein–coated surfaces studied by an extended quartz crystal microbalance technique. Coll Surf B Biointerfaces 11:255–264
Li S-Y, Ramsden JJ, Prenosil JE, Heinzle E (1994) Measurement of adhesion and spreading kinetics of baby hamster kidney and hybridoma cells using an integrated optical method. Biotechnol Prog 10:520–524
Hug TS, Prenosil JE, Morbidelli M (2000) Optical waveguide lightmode spectroscopy as a new method to study adhesion of anchorage-dependent cells as an indicator of metabolic state. Biosens Bioelectron 16:865–874
Taylor AC (1961) Attachment and spreading of cells in culture. Exp Cell Res Suppl 8:154–173
Hanein D et al (1993) Selective interactions of cels with crystal surfaces. J Cell Sci 104:275–288
Ramsden JJ (1995) Optical method for measurement of number and shape of attached cells in real time. Cytometry 19:97–102
Aref A, Horvath R, McColl J, Ramsden, JJ ( 2009) Optical monitoring of stem cell-substratum interactions. J Biomed Opt 14:010501
Aref AR, Jeremy JR (2010) Nanotechnology applied to stem cell- substratum interactions: models and experiment. VDM Verlag, Saarbrücken
Aref A, Horvath R, Ramsden JJ (2010) Spreading kinetics for quantifying cell state during stem cell differentiation. J Biol Phys Chem 10:145–151
Cacace MG, Landau EM, Ramsden JJ (1997) The Hofmeister series: salt and solvent effects on interfacial phenomena. Q Rev Biophys 30:241–278
Shi L, Ardehali R, Caldwell KD, Valint P (2000) Mucin coating on polymeric material surfaces to suppress bacterial adhesion. Coll Surf B 17:229–239
Ramsden JJ, Bachmanova GI, Archakov AI (1994) Kinetic evidence for protein clustering at a surface. Phys Rev E 50:5072–5076
Ramsden JJ, Statist J (1993) Review of new experimental techniques for investigating random sequential adsorption. Physics 73:853–877
Ramsden JJ, Mate M (1998) Journal chemistry Society. Faraday Trans 94:783–788
Levenberg S, Khademhosseini A, Langer R (2006) Embryonic stem cell in tissue engineering. In: Lanza R (ed) Essentials of stem cell biology. Elsevier, San Diego
Gonsalves K, Craig RH, Cato TL, Lakshmi SN (2008) Biomedical nanostructures. Wiley, Hoboken
Revell PA (2006) The biological effects of nanoparticles. Nanotechnol Precept 2:283–298
Solter D, Skreb N, Damjanov I (1970) Extra uterine growth of mouse egg cylinders results in malignant teratoma. Nature 227:503–504
Shvedova AA (2003) Exposure to carbon nanotube material: assessment nanotube cytotoxicity using human keratinocyte cells. J Toxicol Environ Health 66:1909–1926
McKenzie JL (2004) Decreased functions of astrocytes on carbon nanofiber materials. Biomaterials 25:1309–1317
Cui D (2005) Effect of single wall carbon nanotubes on human HEK293 cells. Toxicol Lett 155:73–85
Vickerman V, Blundo J, Chung S, Kamm R (2008) Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. Lap Chip 8(9):1468–1477
Whitesides G, Mc Donald (2002) Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. Acc Chem Res 35(7):491–499
Sia S, Whitesides G (2003) Microfluidic devices fabricated in Poly (dimethylsiloxane) for biological studies. Electrophoresis 24(21):3563–3576
Thiery JP, Hervé Acloque R, Huang YJ, Angela M (2009) Epithelial-mesenchymal transitions in development and disease. Cell 25(139):871–890
Chua KN, Ma J, Thiery JP (2008) Targeted therapies in control of EMT in carcinoma and fibrosis. Drug Discov Today 4:261–267
Valentinuzzi MA (2004) Primer for bioengineering. World Scientific Publishing Co., Inc., Hackensack
Horvath R, Henrik CP, Nina S (2005) Monitoring of living cell attachment and spreading using reverse symmetry waveguide sensing. Appl Phys Lett 86:071101
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Aref, A.R. (2012). Application of Micro/Nanotechnology to Stem Cell Research and Technology. In: Baharvand, H., Aghdami, N. (eds) Advances in Stem Cell Research. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-940-2_10
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