Abstract
To prolong the stage of undifferentiation, human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have traditionally been isolated and cultured using feeder layers, such as mouse embryonic fibroblasts (MEFs) or foreskin fibroblasts, with medium supplemented by fetal bovine serum (FBS). For research purposes, these conditions are preferable and are often referred to as the gold standard. This chapter describes the colony morphology of undifferentiated hESCs and iPSCs cultured with MEFs or human foreskin fibroblasts.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Aguilar-Gallardo C, Poo M, Gomez E, Galan A, Sanchez E, Marques-Mari A, Ruiz V, Medrano J, Riboldi M, Valbuena D, Simon C (2010) Derivation, characterization, differentiation, and registration of seven human embryonic stem cell lines (VAL-3, -4, -5, -6M, -7, -8, and -9) on human feeder. In Vitro Cell Dev Biol Anim 46(3–4):317–326
Amit M, Margulets V, Segev H, Shariki C, Laevsky I, Coleman R, Itskovitz-Eldor J (2003) Human feeder layers for human embryonic stem cells. Biol Reprod 68:2150–2156
Cheng L, Hammond H, Ye Z, Zhan X, Dravid G (2003) Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells 21:131–142
Crook JM, Peura TT, Kravets L, Bosman AG, Buzzard JJ, Horne R, Hentze H, Dunn NR, Zweigerdt R, Chua F, Upshall A, Colman A (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1:490–494
Ellerström C, Strehl R, Moya K, Andersson K, Bergh C, Lundin K, Hyllner J, Semb H (2006) Derivation of a xeno-free human embryonic stem cell line. Stem Cells 24:2170–2176
Genbacev O, Krtolica A, Zdravkovic T, Brunette E, Powell S, Nath A, Caceres E, McMaster M, McDonagh S, Li Y, Mandalam R, Lebkowski J, Fisher SJ (2005) Serum-free derivation of human embryonic stem cell lines on human placental fibroblast feeders. Fertil Steril 83:1517–1529
Hovatta O, Mikkola M, Gertow K, Stromberg AM, Inzunza J, Hreinsson J, Rozell B, Blennow E, Andang M, Ahrlund-Richter L (2003) A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 18:1404–1409
Ilic D, Giritharan G, Zdravkovic T, Caceres E, Genbacev O, Fisher SJ, Krtolica A (2009) Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. Stem Cells Dev 18:1343–1350
Inzunza J, Gertow K, Strömberg MA, Matilainen E, Blennow E, Skottman H, Wolbank S, Ahrlund-Richter L, Hovatta O (2005) Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells. Stem Cells 23:544–549
Richards M, Fong CY, Chan WK, Wong PC, Bongso A (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20:933–936
Simón C, Escobedo C, Valbuena D, Genbacev O, Galan A, Krtolica A, Asensi A, Sánchez E, Esplugues J, Fisher S, Pellicer A (2005) First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions. Fertil Steril 83:246–249
Ström S, Holm F, Bergström R, Strömberg AM, Hovatta O (2010) Derivation of 30 human embryonic stem cell lines. In Vitro Cell Dev Biol Anim 46(3–4):337–344
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Tecirlioglu RT, Nguyen L, Koh K, Trounson AO, Michalska AE (2010) Derivation and maintenance of human embryonic stem cell line on human adult skin fibroblast feeder cells in serum replacement medium. In Vitro Cell Dev Biol Anim 46(3–4):231–235
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147 [erratum in Science 1998;282:1827]
Valbuena D, Galán A, Sánchez E, Poo ME, Gómez E, Sánchez-Luengo S, Melguizo D, García A, Ruiz V, Moreno R, Pellicer A, Simón C (2006) Derivation and characterization of three new Spanish human embryonic stem cell lines (VAL -3 -4 -5) on human feeder and in serum-free conditions. Reprod Biomed Online 13:875–886
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Amit, M., Itskovitz-Eldor, J. (2012). Morphology of Human Embryonic and Induced Pluripotent Stem Cell Colonies Cultured with Feeders. In: Amit, M., Itskovitz-Eldor, J. (eds) Atlas of Human Pluripotent Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-548-0_2
Download citation
DOI: https://doi.org/10.1007/978-1-61779-548-0_2
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-547-3
Online ISBN: 978-1-61779-548-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)