Abstract
The adult human heart has limited regenerative capacity. Loss of myocardium, most commonly through ischemic injury, results in the clinical syndrome of heart failure. Current therapies are largely palliative and the only treatment for end-stage heart failure with established long-term efficacy is transplantation. Consequently, there has been significant interest in developing novel regenerative strategies. Use of human embryonic stem cell (hESC) therapy is complicated by ethical and technical problems including possible host immune rejection of transplanted cells. Induced pluripotent stem cells (iPSCs) share many properties with hESC but do not require the ethically problematic destruction of embryos. Furthermore, as iPSC can be generated from somatic cells of specific patients, it is theoretically possible to perform allogenic cell transplantation or to create patient-specific in vitro disease models using iPSC-derived cardiac myocytes. In this chapter, we discuss the developmental potential of hESC and iPSC for cardiomyogenesis, including existing methods for differentiation and maturation.
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References
Abdel-Latif A, Bolli R, Tleyjeh IM et al (2007) Adult bone marrow-derived cells for cardiac repair: a systematic review and meta-analysis. Arch Intern Med 167:989–997
Abdul Kadir S, Ali N, Mioulane M et al (2009) Embryonic stem cell-derived cardiomyocytes as a model to study fetal arrhythmia related to maternal disease. J Cell Mol Med 13:3730–3741
Abraham MR, Henrikson CA, Tung L et al (2005) Antiarrhythmic engineering of skeletal myoblasts for cardiac transplantation. Circ Res 97:159–167
Adewumi O, Aflatoonian B, Ahrlund-Richter L et al (2007) Characterization of human embryonic stem cell lines by the international stem cell initiative. Nat Biotechnol 25:803–816
Ameen C, Strehl R, Bjorquist P et al (2008) Human embryonic stem cells: current technologies and emerging industrial applications. Crit Rev Oncol Hematol 65:54–80
Baharvand H, Piryaei A, Rohani R et al (2006) Ultrastructural comparison of developing mouse embryonic stem cell- and in vivo-derived cardiomyocytes. Cell Biol Int 30:800–807
Bassani JW, Yuan W, Bers DM (1995) Fractional SR Ca release is regulated by trigger Ca and SR Ca content in cardiac myocytes. Am J Physiol 268:C1313–C1319
Beard NA, Laver DR, Dulhunty AF (2004) Calsequestrin and the calcium release channel of skeletal and cardiac muscle. Prog Biophys Mol Biol 85:33–69
Bearzi C, Rota M, Hosoda T et al (2007) Human cardiac stem cells. Proc Natl Acad Sci USA 104:14068–14073
Beltrami AP, Barlucchi L, Torella D et al (2003) Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 114:763–776
Bergmann O, Bhardwaj RD, Bernard S et al (2009) Evidence for cardiomyocyte renewal in humans. Science 324:98–102
Bers DM (2002) Cardiac excitation-contraction coupling. Nature 415:198–205
Bers DM, Despa S (2009) Na+ transport in cardiac myocytes; Implications for excitation-contraction coupling. IUBMB Life 61:215–221
Bers DM, Despa S, Bossuyt J (2006) Regulation of Ca2+ and Na+ in normal and failing cardiac myocytes. Ann N Y Acad Sci 1080:165–177
Bi W, Drake CJ, Schwarz JJ (1999) The transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 and VEGF. Dev Biol 211:255–267
Boheler K, Czyz J, Tweedie D et al (2002) Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res 91:189–201
Bonneux L, Barendregt JJ, Meeter K et al (1994) Estimating clinical morbidity due to ischemic heart disease and congestive heart failure: the future rise of heart failure. Am J Public Health 84:20–28
Brand NJ, Dabhade N, Yacoub M et al (1991) Determination of the 5′ exon structure of the human cardiac alpha-myosin heavy chain gene. Biochem Biophys Res Commun 179:1255–1258
Brette F, Orchard C (2003) T-tubule function in mammalian cardiac myocytes. Circ Res 92:1182–1192
Brette F, Orchard C (2007) Resurgence of cardiac t-tubule research. Physiology (Bethesda) 22:167–173
Burridge PW, Anderson D, Priddle H et al (2007) Improved human embryonic stem cell embryoid body homogeneity and cardiomyocyte differentiation from a novel V-96 plate aggregation system highlights interline variability. Stem Cells 25:929–938
Cao F, Wagner RA, Wilson KD et al (2008) Transcriptional and functional profiling of human embryonic stem cell-derived cardiomyocytes. PLoS One 3:e3474
Carvajal-Vergara X, Sevilla A, D’Souza SL et al (2010) Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome. Nature 465:808–812
Caspi O, Itzhaki I, Kehat I et al (2009) In vitro electrophysiological drug testing using human embryonic stem cell derived cardiomyocytes. Stem Cells Dev 18:161–172
Chambers I, Colby D, Robertson M et al (2003) Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113:643–655
Chen QZ, Ishii H, Thouas GA et al (2010) An elastomeric patch derived from poly(glycerol sebacate) for delivery of embryonic stem cells to the heart. Biomaterials 31:3885–3893
Chin M, Mason M, Xie W et al (2009) Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell 5:111–123
Clegg AJ, Scott DA, Loveman E et al (2006) Clinical and cost-effectiveness of left ventricular assist devices as a bridge to heart transplantation for people with end-stage heart failure: a systematic review and economic evaluation. Eur Heart J 27:2929–2938
Cohen ED, Wang Z, Lepore JJ et al (2007) Wnt/beta-catenin signaling promotes expansion of Isl-1-positive cardiac progenitor cells through regulation of FGF signaling. J Clin Invest 117:1794–1804
de Boer TP, van Veen TAB, Houtman MJC et al (2006) Inhibition of cardiomyocyte automaticity by electrotonic application of inward rectifier current from Kir2.1 expressing cells. Med Biol Eng Comput 44:537–542
De Miguel MP, Fuentes-Julian S, Alcaina Y (2010) Pluripotent stem cells: origin, maintenance and induction. Stem Cell Rev 6:633–649
Deng J, Shoemaker R, Xie B et al (2009) Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming. Nat Biotechnol 27:353–360
Desplantez T, Dupont E, Severs NJ et al (2007) Gap junction channels and cardiac impulse propagation. J Membr Biol 218:13–28
DiFrancesco D (2010) The role of the funny current in pacemaker activity. Circ Res 106:434–446
Dolnikov K, Shilkrut M, Zeevi-Levin N et al (2006) Functional properties of human embryonic stem cell-derived cardiomyocytes: intracellular Ca2+ handling and the role of sarcoplasmic reticulum in the contraction. Stem Cells 24:236–245
Durocher D, Charron F, Warren R et al (1997) The cardiac transcription factors Nkx2-5 and GATA-4 are mutual cofactors. EMBO J 16:5687–5696
Eldstrom J, Choi WS, Steele DF et al (2003) SAP97 increases Kv1.5 currents through an indirect N-terminal mechanism. FEBS Lett 547:205–211
Filipczyk AA, Passier R, Rochat A et al (2007) Regulation of cardiomyocyte differentiation of embryonic stem cells by extracellular signalling. Cell Mol Life Sci 64:704–718
Flaherty MP, Abdel-Latif A, Li Q et al (2008) Noncanonical Wnt11 signaling is sufficient to induce cardiomyogenic differentiation in unfractionated bone marrow mononuclear cells. Circulation 117:2241–2252
Fu JD, Li J, Tweedie D et al (2006) Crucial role of the sarcoplasmic reticulum in the developmental regulation of Ca2+ transients and contraction in cardiomyocytes derived from embryonic stem cells. FASEB J 20:181–183
Fu JD, Jiang P, Rushing S et al (2010) Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes. Stem Cells Dev 19:773–782
Garg V, Kathiriya IS, Barnes R et al (2003) GATA4 mutations cause human congenital heart defects and reveal an interaction with TBX5. Nature 424:443–447
Gersh BJ, Simari RD, Behfar A et al (2009) Cardiac cell repair therapy: a clinical perspective. Mayo Clin Proc 84:876–892
Graichen R, Xu X, Braam SR et al (2008) Enhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK. Differentiation 76:357–370
Gridelli B, Remuzzi G (2000) Strategies for making more organs available for transplantation. N Engl J Med 343:404–410
Habib M, Caspi O, Gepstein L (2008) Human embryonic stem cells for cardiomyogenesis. J Mol Cell Cardiol 45:462–474
Hattori F, Chen H, Yamashita H et al (2010) Nongenetic method for purifying stem cell-derived cardiomyocytes. Nat Methods 7:61–66
He JQ, Ma Y, Lee Y et al (2003) Human embryonic stem cells develop into multiple types of cardiac myocytes: action potential characterization. Circ Res 93:32–39
Hescheler J, Fleischmann BK, Lentini S et al (1997) Embryonic stem cells: a model to study structural and functional properties in cardiomyogenesis. Cardiovasc Res 36:149–162
Hu BY, Weick JP, Yu J et al (2010) Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency. Proc Natl Acad Sci USA 107:4335–4340
Ieda M, Fu JD, Delgado-Olguin P et al (2010) Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 142:375–386
Itskovitz-Eldor J, Schuldiner M, Karsenti D et al (2000) Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med 6:88–95
Kattman SJ, Huber TL, Keller GM (2006) Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages. Dev Cell 11:723–732
Kehat I, Kenyagin-Karsenti D, Snir M et al (2001) Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest 108:407–414
Kleber AG, Rudy Y (2004) Basic mechanisms of cardiac impulse propagation and associated arrhythmias. Physiol Rev 84:431–488
Kolossov E, Lu Z, Drobinskaya I et al (2005) Identification and characterization of embryonic stem cell-derived pacemaker and atrial cardiomyocytes. FASEB J 19:577–579
Kong CW, Akar FG, Li RA (2010) Translational potential of human embryonic and induced pluripotent stem cells for myocardial repair: insights from experimental models. Thromb Haemost 104:30–38
Kubalak SW, Miller-Hance WC, O’Brien TX et al (1994) Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis. J Biol Chem 269:16961–16970
Kuratomi S, Ohmori Y, Ito M et al (2009) The cardiac pacemaker-specific channel Hcn4 is a direct transcriptional target of MEF2. Cardiovasc Res 83:682–687
Laflamme MA, Chen KY, Naumova AV et al (2007) Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol 25:1015–1024
Lee J, Terracciano CM (2010) Cell therapy for cardiac repair. Br Med Bull 94:65–80
Lev S, Kehat I, Gepstein L (2005) Differentiation pathways in human embryonic stem cell-derived cardiomyocytes. Ann N Y Acad Sci 1047:50–65
Lieu DK, Liu J, Siu CW et al (2009) Absence of transverse tubules contributes to non-uniform Ca(2+) wavefronts in mouse and human embryonic stem cell-derived cardiomyocytes. Stem Cells Dev 18:1493–1500
Liu J, Fu JD, Siu CW et al (2007) Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation. Stem Cells 25:3038–3044
Lo B, Parham L (2009) Ethical issues in stem cell research. Endocr Rev 30:204–213
Ludwig T, Thomson J (2007) Defined, feeder-independent medium for human embryonic stem cell culture. Curr Protoc Stem Cell Biol Chapter 1:Unit 1C.2
Lukyanenko V, Gyorke I, Gyorke S (1996) Regulation of calcium release by calcium inside the sarcoplasmic reticulum in ventricular myocytes. Pflugers Arch 432:1047–1054
Macera MJ, Szabo P, Wadgaonkar R et al (1992) Localization of the gene coding for ventricular myosin regulatory light chain (MYL2) to human chromosome 12q23-q24.3. Genomics 13:829–831
Marcellini S, Technau U, Smith JC et al (2003) Evolution of Brachyury proteins: identification of a novel regulatory domain conserved within bilateria. Dev Biol 260:352–361
Mauritz C, Schwanke K, Reppel M et al (2008) Generation of functional murine cardiac myocytes from induced pluripotent stem cells. Circulation 118:507–517
McMurray JJ, Stewart S (2000) Epidemiology, aetiology, and prognosis of heart failure. Heart 83:596–602
Menasche P, Hagege AA, Scorsin M et al (2001) Myoblast transplantation for heart failure. Lancet 357:279–280
Messina E, De Angelis L, Frati G et al (2004) Isolation and expansion of adult cardiac stem cells from human and murine heart. Circ Res 95:911–921
Mitsui K, Tokuzawa Y, Itoh H et al (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113:631–642
Moore JC, Fu J, Chan YC et al (2008) Distinct cardiogenic preferences of two human embryonic stem cell (hESC) lines are imprinted in their proteomes in the pluripotent state. Biochem Biophys Res Commun 372:553–558
Moretti A, Caron L, Nakano A et al (2006) Multipotent embryonic isl1+ progenitor cells lead to cardiac, smooth muscle, and endothelial cell diversification. Cell 127:1151–1165
Moretti A, Bellin M, Welling A et al (2010) Patient-specific induced pluripotent stem-cell models for long-QT syndrome. N Engl J Med 363:1397–1409
Muller M, Fleischmann BK, Selbert S et al (2000) Selection of ventricular-like cardiomyocytes from ES cells in vitro. FASEB J 14:2540–2548
Mummery C, Ward-van Oostwaard D, Doevendans P et al (2003) Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells. Circulation 107:2733–2740
Murry CE, Soonpaa MH, Reinecke H et al (2004) Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 428:664–668
Narazaki G, Uosaki H, Teranishi M et al (2008) Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells. Circulation 118:498–506
Nelson TJ, Martinez-Fernandez A, Yamada S et al (2009) Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells. Circulation 120:408–416
Niwa H, Miyazaki J, Smith AG (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24:372–376
Nostro MC, Cheng X, Keller GM et al (2008) Wnt, activin, and BMP signaling regulate distinct stages in the developmental pathway from embryonic stem cells to blood. Cell Stem Cell 2:60–71
Novak K (2010) Stem cell therapies: California Dreamin’? Cell 140:10–12
Olson EN (2001) Development. The path to the heart and the road not taken. Science 291:2327–2328
Olson EN (2004) A decade of discoveries in cardiac biology. Nat Med 10:467–474
Orlic D, Kajstura J, Chimenti S et al (2001) Bone marrow cells regenerate infarcted myocardium. Nature 410:701–705
Osafune K, Caron L, Borowiak M et al (2008) Marked differences in differentiation propensity among human embryonic stem cell lines. Nat Biotechnol 26:313–315
Otsuji TG, Minami I, Kurose Y et al (2010) Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: qualitative effects on electrophysiological responses to drugs. Stem Cell Res 4:201–213
Pal R, Khanna A (2007) Similar pattern in cardiac differentiation of human embryonic stem cell lines, BG01V and ReliCellhES1, under low serum concentration supplemented with bone morphogenetic protein-2. Differentiation 75:112–122
Pantalos GM, Marks JD, Riebman JB et al (1988) Hemodynamic and energetic assessment of calves implanted with a left-ventricular assist device (Lvad). Int J Artif Organs 11:119–126
Passier R, Oostwaard DW, Snapper J et al (2005) Increased cardiomyocyte differentiation from human embryonic stem cells in serum-free cultures. Stem Cells 23:772–780
Passier R, van Laake LW, Mummery CL (2008) Stem-cell-based therapy and lessons from the heart. Nature 453:322–329
Pekkanen-Mattila M, Kerkela E, Tanskanen JM et al (2009) Substantial variation in the cardiac differentiation of human embryonic stem cell lines derived and propagated under the same conditions – a comparison of multiple cell lines. Ann Med 41:360–370
Pekkanen-Mattila M, Chapman H, Kerkela E et al (2010a) Human embryonic stem cell-derived cardiomyocytes: demonstration of a portion of cardiac cells with fairly mature electrical phenotype. Exp Biol Med (Maywood) 235:522–530
Pekkanen-Mattila M, Pelto-Huikko M, Kujala V et al (2010b) Spatial and temporal expression pattern of germ layer markers during human embryonic stem cell differentiation in embryoid bodies. Histochem Cell Biol 133:595–606
Qyang Y, Martin-Puig S, Chiravuri M et al (2007) The renewal and differentiation of Isl1+ cardiovascular progenitors are controlled by a Wnt/beta-catenin pathway. Cell Stem Cell 1:165–179
Rubart M, Soonpaa MH, Nakajima H et al (2004) Spontaneous and evoked intracellular calcium transients in donor-derived myocytes following intracardiac myoblast transplantation. J Clin Invest 114:775–783
Saito Y, Nakao K, Arai H et al (1989) Augmented expression of atrial natriuretic polypeptide gene in ventricle of human failing heart. J Clin Invest 83:298–305
Saric T, Frenzel LP, Hescheler J (2008) Immunological barriers to embryonic stem cell-derived therapies. Cells Tissues Organs 188:78–90
Sartiani L, Bettiol E, Stillitano F et al (2007) Developmental changes in cardiomyocytes differentiated from human embryonic stem cells: a molecular and electrophysiological approach. Stem Cells 25:1136–1144
Satin J, Kehat I, Caspi O et al (2004) Mechanism of spontaneous excitability in human embryonic stem cell derived cardiomyocytes. J Physiol 559:479–496
Satin J, Itzhaki I, Rapoport S et al (2008) Calcium handling in human embryonic stem cell-derived cardiomyocytes. Stem Cells 26:1961–1972
Sato N, Meijer L, Skaltsounis L et al (2004) Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 10:55–63
Schuldiner M, Yanuka O, Itskovitz-Eldor J et al (2000) Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc Natl Acad Sci USA 97:11307–11312
Selvaraj V, Plane JM, Williams AJ et al (2010) Switching cell fate: the remarkable rise of induced pluripotent stem cells and lineage reprogramming technologies. Trends Biotechnol 28:214–223
Shannon TR, Ginsburg KS, Bers DM (2000) Potentiation of fractional sarcoplasmic reticulum calcium release by total and free intra-sarcoplasmic reticulum calcium concentration. Biophys J 78:334–343
Snir M, Kehat I, Gepstein A et al (2003) Assessment of the ultrastructural and proliferative properties of human embryonic stem cell-derived cardiomyocytes. Am J Physiol Heart Circ Physiol 285:H2355–H2363
Song LS, Guatimosim S, Gomez-Viquez L et al (2005) Calcium biology of the transverse tubules in heart. Ann N Y Acad Sci 1047:99–111
Tada M, Kirchberger MA, Repke DI et al (1974) The stimulation of calcium transport in cardiac sarcoplasmic reticulum by adenosine 3′:5′-monophosphate-dependent protein kinase. J Biol Chem 249:6174–6180
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676
Takahashi T, Lord B, Schulze PC et al (2003) Ascorbic acid enhances differentiation of embryonic stem cells into cardiac myocytes. Circulation 107:1912–1916
Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Tanaka T, Tohyama S, Murata M et al (2009) In vitro pharmacologic testing using human induced pluripotent stem cell-derived cardiomyocytes. Biochem Biophys Res Commun 385:497–502
Tansley P, Yacoub M, Rimoldi O et al (2004) Effect of left ventricular assist device combination therapy on myocardial blood flow in patients with end-stage dilated cardiomyopathy. J Heart Lung Transplant 23:1283–1289
Taylor DO, Edwards LB, Boucek MM et al (2007) Registry of the International Society for Heart and Lung Transplantation: twenty-fourth official adult heart transplant report – 2007. J Heart Lung Transplant 26:769–781
Thomson JA, Itskovitz-Eldor J, Shapiro SS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147
Townsend PJ, Farza H, MacGeoch C et al (1994) Human cardiac troponin T: identification of fetal isoforms and assignment of the TNNT2 locus to chromosome 1q. Genomics 21:311–316
Tran TH, Wang X, Browne C et al (2009) Wnt3a-induced mesoderm formation and cardiomyogenesis in human embryonic stem cells. Stem Cells 27:1869–1878
Ueno S, Weidinger G, Osugi T et al (2007) Biphasic role for Wnt/beta-catenin signaling in cardiac specification in zebrafish and embryonic stem cells. Proc Natl Acad Sci USA 104:9685–9690
Viatchenko-Karpinski S, Fleischmann BK, Liu Q et al (1999) Intracellular Ca2+ oscillations drive spontaneous contractions in cardiomyocytes during early development. Proc Natl Acad Sci USA 96:8259–8264
Vidarsson H, Hyllner J, Sartipy P (2010) Differentiation of human embryonic stem cells to cardiomyocytes for in vitro and in vivo applications. Stem Cell Rev 6:108–120
Vierbuchen T, Ostermeier A, Pang ZP et al (2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463:1035–1041
Willems E, Bushway PJ, Mercola M (2009) Natural and synthetic regulators of embryonic stem cell cardiogenesis. Pediatr Cardiol 30:635–642
Wollert KC, Drexler H (2010) Cell therapy for the treatment of coronary heart disease: a critical appraisal. Nat Rev Cardiol 7:204–215
Wu X, Ding S, Ding Q et al (2004) Small molecules that induce cardiomyogenesis in embryonic stem cells. J Am Chem Soc 126:1590–1591
Wu SM, Fujiwara Y, Cibulsky SM et al (2006) Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart. Cell 127:1137–1150
Xu C, Police S, Rao N et al (2002) Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ Res 91:501–508
Xu XQ, Graichen R, Soo SY et al (2008) Chemically defined medium supporting cardiomyocyte differentiation of human embryonic stem cells. Differentiation 76:958–970
Xue T, Cho H, Akar F et al (2005) Functional integration of electrically active cardiac derivatives from genetically engineered human embryonic stem cells with quiescent recipient ventricular cardiomyocytes: insights into the development of cell-based pacemakers. Circulation 111:11–20
Yamada S, Nelson TJ, Crespo-Diaz RJ et al (2008) Embryonic stem cell therapy of heart failure in genetic cardiomyopathy. Stem Cells 26:2644–2653
Yang L, Soonpaa MH, Adler ED et al (2008) Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 453:524–528
Yao S, Chen S, Clark J et al (2006) Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc Natl Acad Sci USA 103:6907–6912
Ying QL, Nichols J, Chambers I et al (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115:281–292
Yoshida Y, Yamanaka S (2010) Recent stem cell advances: induced pluripotent stem cells for disease modeling and stem cell-based regeneration. Circulation 122:80–87
Yu J, Vodyanik M, Smuga-Otto K et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920
Zaffran S, Frasch M (2002) Early signals in cardiac development. Circ Res 91:457–469
Zhang P, Li J, Tan Z et al (2008) Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood 111:1933–1941
Zhang J, Wilson GF, Soerens AG et al (2009) Functional cardiomyocytes derived from human induced pluripotent stem cells. Circ Res 104:e30–e41
Zwi L, Caspi O, Arbel G et al (2009) Cardiomyocyte differentiation of human induced pluripotent stem cells. Circulation 120:1513–1523
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Rao, C., Ali, N.N., Athanasiou, T., Terracciano, C., Harding, S. (2011). Phenotype and Developmental Potential of Cardiomyocytes from Induced Pluripotent Stem Cells and Human Embryonic Stem Cells. In: Ainscough, J., Yamanaka, S., Tada, T. (eds) Nuclear Reprogramming and Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-225-0_16
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