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Methods for Inducing Pluripotency

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Regenerating the Heart

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

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Abstract

Induced pluripotent stem (iPS) cells are embryonic stem-like cells produced by forcing expression of a minimal number of key factors in differentiated somatic cells. In many ways, they are indistinguishable from embryonic stem cells in that they can differentiate into any cell type in the body. This development has led to worldwide excitement over the possibility to develop cell-based therapies for a variety of degenerative diseases using cells derived from and thus genetically matched to the patient they are aimed to treat. This chapter reviews the scientific foundation that has led to the ability to create iPS cells and the current methods used to make them, as well as the studies that have been conducted to help decipher the molecular pathways involved. The evolution of steps developed in recent years to improve both the efficiency and the safety of the process for clinical and in vitro diagnostic use is also presented.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Department of Biology and Biotechnology, Bioengineering Institute, Worcester Polytechnic Institute, Worcester, MA, 01609, USA

    Raymond L. Page

  2. Cellthera, Inc., Southbridge, MA, 01550, USA

    Raymond L. Page

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  2. Christopher Malcuit
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  3. Tanja Dominko
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Correspondence to Raymond L. Page .

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Editors and Affiliations

  1. Health Sciences Center, Dept. Physiology & Biophysics, Stony Brook University, Stony Brook, 11794-8661, New York, USA

    Ira S. Cohen

  2. Worcester Polytechnic Institute, Institute Rd. 100, Worcester, 01609-2280, Massachusetts, USA

    Glenn R. Gaudette

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© 2011 Springer Science+Business Media, LLC

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Page, R.L., Malcuit, C., Dominko, T. (2011). Methods for Inducing Pluripotency. In: Cohen, I., Gaudette, G. (eds) Regenerating the Heart. Stem Cell Biology and Regenerative Medicine. Humana Press. https://doi.org/10.1007/978-1-61779-021-8_13

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