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Measuring Oxidative Stress in Cell Cultures, Animals and Humans: Analysis and Validation of Oxidatively Damaged DNA

  • Hanna L. Karlsson
  • Lennart MöllerEmail author
Chapter
Part of the Oxidative Stress in Applied Basic Research and Clinical Practice book series (OXISTRESS)

Abstract

An association between oxidative stress and different diseases has been described in the literature, but there are obvious shortcomings with methods available to assess oxidative stress. This chapter focuses on the analysis of markers for oxidative stress with special attention on biomarkers for analysis of oxidatively damaged DNA. Such markers have often been used, but the levels in healthy and diseased subjects show high variation in the literature. Therefore, various efforts that have been undertaken, or are ongoing, which aim to validate different methods for analysis of oxidatively damaged DNA, will be discussed. It is concluded that measuring oxidatively damaged DNA is a useful biomarker for oxidative stress and that further validation of this biomarker is important. Oxidatively damaged DNA can be used as a marker to monitor oxidative stress in different diseases or following different exposures. However, it may also have the potential to act as a biomarker of disease development risk and may be used clinically to assess efficacy of therapy.

Keywords

Comet assay DNA damage Oxidative stress Proteins 

Notes

Acknowledgements

The authors of this paper are also partners in ECNIS (Environmental Cancer Risk, Nutrition and Individual Susceptibility), a network of excellence operating within the European Union 6th framework program, priority 5: “Food Quality and Safety” (Contract No. 513943), as well as ESCODD and ECVAG.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Unit for Analytical Toxicology, Department of Biosciences and NutritionNovum, Karolinska InstitutetStockholmSweden

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