Experimental Models to Study Cigarette Smoke-Induced Oxidative Stress In Vitro and In Vitro in Preclinical Models, and in Smokers and Patients with Airways Disease

  • Hongwei Yao
  • Irfan RahmanEmail author
Part of the Oxidative Stress in Applied Basic Research and Clinical Practice book series (OXISTRESS)


Oxidative stress is one of the important hallmarks of various chronic inflammatory lung diseases including chronic obstructive pulmonary disease (COPD). Cigarette smoke contains high concentrations of oxidants/free radicals and different chemical compounds including reactive aldehydes, quinones, and semiquinones. It has been shown that cigarette smoke is the major risk factor for the development of COPD by inducing oxidative stress and lung inflammation. Therefore, studying cigarette smoke-induced oxidative stress in vitro and in vivo models is useful for understanding the pathogenesis of chronic inflammatory lung diseases, and developing the potential antioxidants and anti-inflammatory therapies to intervene in the progression of COPD/emphysema. In this chapter, we discuss these in vitro and in vivo experimental models induced by cigarette smoke exposures. Furthermore, human samples, such as exhaled breath condensate, induced sputum, and bronchoalveolar lavage from smokers and patients with COPD, are also useful for investigating the association of oxidative stress with the progression of COPD. However, all these models cannot mimic the exact phenotypic alterations seen in human smokers and COPD patients. Oxidative stress biomarkers are also used to monitor the progression and prognosis of cigarette smoke-induced diseases, such as COPD.


Biomarkers Cigarette smoke COPD Experimental models Oxidative stress 



This study was supported by the NIH R01-HL085613 and NIEHS Environmental Health Science Center grant ES-01247.


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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Environmental Medicine, Lung Biology and Disease ProgramUniversity of Rochester Medical CenterRochesterUSA

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