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Capillary Electrophoresis of Carbohydrates pp 237–267Cite as

Monosaccharide Compositional Analysis of Glycoproteins and Glycolipids: Utility in the Diagnosis/Prognosis of Diseases

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Abstract

Different analytical methods have been developed to effectively and accurately determine monosaccharide composition of glycoproteins and glycolipids in biological and biomedical samples. These methods included high-performance anion-exchange chromatography with pulse amperometric detection (HPAEC-PAD), high-performance liquid chromatography with fluorescence detection (HPLC-FL), and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This chapter reviews the development of these methods, and evaluates their potential application in the diagnosis or prognosis of diseases.

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Abbreviations

ABEE:

p-aminobenzoic acid ethyl ester

AMAC:

8-aminoacridone

ANDS:

7-aminonaphthalene-1,3-disulfonic acid

ANS:

2-aminonaphthalene-1-sulfonic acid

ANTS:

8-aminonaphthalene-1,3,6-trisulfonic acid

APTS:

1-aminopyrene-3,6,8-trisulfonic acid

CE:

capillary electrophoresis

CEC:

capillary electrochromatography

CE-LIF:

capillary electrophoresis with laser-induced fluorescence detection

CGE:

capillary gel electrophoresis

CZE:

capillary zone electrophoresis

DMB:

1,2-diamino-4,5-methylenedioxybenzene

EOF:

electro-osmotic flow

Gal:

galactose

GalN:

galactosamine

GalNAc:

N-acetylgalactosamine

GCC:

graphitized carbon column

Glc:

glucose

GlcNAc:

N-acetylglucosamine

GlcN:

glucosamine

GlcNc:

N-acetylglucosamine

HCl:

hydrochloric acid

HPAEC-PAD:

high-performance anion-exchange chromatography with pulse amperometric detection

HPLC-FL:

high-performance liquid chromatography with fluorescence detection

HPLC-MS:

high-performance liquid chromatography with mass spectrometry

Man:

mannose

ManN:

mannosamine

MECK:

micellar electrokinetic chromatography

MRM:

multiple reaction monitoring

NeuAc:

N-acetylneuraminic acid

NeuGc:

N-glycolylneuraminic acid

OPD:

O-phenyllenediamine 2 HCl

PAD:

pulsed amperometric detector

TFA:

trifluoroacetic acid.

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Acknowledgment

This work was supported by the Indiana Metabolomics and Cytomics Initiative (METACyt), funded by a grant from Eli Lilly Endowment.

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  1. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas, 79409-1061, USA

    Yehia Mechref

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  1. Yehia Mechref
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Correspondence to Yehia Mechref .

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  1. Dipto. Biologia, Università di Modena e Reggio Emilia, Via Campi 213/D, Modena, 41100, Italy

    Nicola Volpi

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Mechref, Y. (2011). Monosaccharide Compositional Analysis of Glycoproteins and Glycolipids: Utility in the Diagnosis/Prognosis of Diseases. In: Volpi, N. (eds) Capillary Electrophoresis of Carbohydrates. Humana Press. https://doi.org/10.1007/978-1-60761-875-1_10

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