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Analysis of Simple Carbohydrates by Capillary Electrophoresis and Capillary Electrophoresis–Mass Spectrometry

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Capillary Electrophoresis of Carbohydrates

Abstract

An overview of the application of capillary electrophoresis and ­capillary electrophoresis–mass spectrometry in the analysis of simple carbohydrates without any previous derivatization step is given. Besides electrolyte systems for ­carbohydrate separation, detection techniques employed in capillary electrophoresis, such as ­spectrophotometric detection, electrochemical detection, and mass spectrometric ­detection, are discussed, as are less common detection techniques. Thus, the chapter focuses on the assessment of the strong and weak points of these ­detection ­techniques for carbohydrate analysis. A table included in this chapter gives a ­comprehensive listing of capillary electrophoresis and capillary ­electrophoresis–mass spectrometry ­applications in the fields of carbohydrate analysis published within the last decade.

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Abbreviations

AU:

absorbence units

CAPS:

3-cyclohexylamino-1-propane-sulfonic acid

CE:

capillary electrophoresis

CTAB:

cetyltrimethylammonium bromide

CTAH:

cetyltrimethylammonium hydroxide

EOF:

electroendoosmotic flow

FT-IR:

Fourier transform infrared spectroscopy

GC:

gas chromatography

HDB:

1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene hexadimethrine bromide)

HPLC:

high-performance liquid chromatography

LOD:

limit of detection

LOQ:

limit of quantification

MTAB:

myristylmethylammonium bromide

NAA:

1-naphthylacetic acid

PAD:

pulsed amperometric detection

PDC:

2,6-pyridinedicarboxylic acid

PNP:

p-nitrophenol

SIM:

selected ion monitoring

TTAOH:

tetradecyltrimethylammonium hydroxide

TIC:

total ion current

TMP:

thermomechanical pulp

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Johannes Kepler-University Linz, Altenbergerstrasse 69, A-4040, Linz, Austria

    Christian W. Klampfl

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  1. Christian W. Klampfl
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  2. Markus Himmelsbach
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  3. Wolfgang Buchberger
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Correspondence to Christian W. Klampfl .

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  1. Dipto. Biologia, Università di Modena e Reggio Emilia, Via Campi 213/D, Modena, 41100, Italy

    Nicola Volpi

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Klampfl, C.W., Himmelsbach, M., Buchberger, W. (2011). Analysis of Simple Carbohydrates by Capillary Electrophoresis and Capillary Electrophoresis–Mass Spectrometry. In: Volpi, N. (eds) Capillary Electrophoresis of Carbohydrates. Humana Press. https://doi.org/10.1007/978-1-60761-875-1_1

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