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Making Friends in Out-of-the- Way Places: How Cells of the Immune System Get Together and How They Conduct Their Business as Revealed by Intravital Imaging

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National Institute of Allergy and Infectious Diseases, NIH

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Abstract

During embryonic and early post-natal development, many cells migrate over substantial distances, changing their spatial relationship to other cells of the same or distinct differentiation state and engaging in cross-talk that helps establish the tissue and organ structure of the adult. However, once this body plan has been established, such active migration and positional exchange is rare in the undiseased state. Even in epithelial tissues that undergo constant renewal, there is an absence of free cell movement; rather, underlying cells replicate and push the older cells to the surface (skin) or apex (villus) where they are sloughed, having followed a fixed path of movement from birth to death.

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Acknowledgements

The authors thank other members of the LBS for their help in the conduct of some of the studies described here, especially Ina Ifrim for her efforts as we introduced 2P instruments into our research program. We also thank our external collaborators on studies mentioned here (especially Nicolas Glaichenhaus, Maria Rescigno, Alan Sher and Antonio Rothfuchs) for working with us to develop the stories we have described in this review. Thanks also to Owen Schwartz of the Biological Imaging Facility of the RTB, NIAID, NIH for his help, advice, and assistance in some of the studies from our laboratory, to Thorsten Mempel and Uli von Andrian for their generous assistance when we were developing the 2P intravital imaging methods used in our laboratory, and to the many colleagues who have created and generously made available genetically modified mouse strains critical to our investigations. This research was supported by the Intramural Research Program of NIAID, NIH.

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Authors and Affiliations

  1. Laboratory of Immunology, Lymphocyte Biology Section, Program in Systems Immunology and Infectious Disease Modeling, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA

    Ronald N. Germain, Marcello Chieppa, Jackson G. Egen, Masaru Ishii & Hai Qi

  2. INSERM U344, IPMC, CNRS, Université de Nice-Sophia-Antipolis., Valbonne, France

    Marc Bajénoff

  3. Research Center, Novartis Vaccines and Diagnostics, Via Fiorentina 1, Siena, Italy

    Flora Castellino

  4. Division of Pediatric Hematology/Oncology, Rainbow Babies & Children’s Hospital, Case Western Reserve University School of Medicine, Cleveland, OH, USA

    Alex Y. C. Huang

  5. Biological Imaging Facility, Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA

    Lily Y. Koo

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  1. Ronald N. Germain
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  1. Dept. Health & Human Services, National Institute of Health, Rockledge Drive 6610, Bethesda, 20892, Maryland, USA

    Vassil St. Georgiev

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Germain, R.N. et al. (2010). Making Friends in Out-of-the- Way Places: How Cells of the Immune System Get Together and How They Conduct Their Business as Revealed by Intravital Imaging. In: Georgiev, V. (eds) National Institute of Allergy and Infectious Diseases, NIH. Infectious Disease. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-512-5_21

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