In Vitro Performance and Analysis of Combination Anti-infective Evaluations

  • Robert W. Buckheit
  • R. Dwayne Lunsford
Part of the Infectious Disease book series (ID)

The evaluation of the activity of combinations of two or more anti-infective compounds has gained signifi cant prominence in light of the innate ability of many infectious organisms to rapidly acquire drug resistance. Pathogens react to the administration of anti-infective agents by the outgrowth of preexisting infectious clones with resistance-engendering mutations and by the accumulation of new mutations to allow an escape from the suppressive effects of therapeutic drug regimens (1). Resistance emerges through the error-prone mechanisms of the replicative machinery and through the transmission of resistance elements (2, 3), rendering monotherapeutic drug strategies problematic. Combination chemotherapy signifi cantly decreases the risk that resistance will arise. In addition, a combination chemotherapy may ameliorate toxicity by permitting lower, less toxic, or nontoxic concentrations of synergistic drugs to be utilized.


Dose Response Curve Antimicrob Agent Combination Index Infectious Organism Fractional Inhibitory Concentration Index 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Robert W. Buckheit
    • 1
  • R. Dwayne Lunsford
    • 1
  1. 1.ImQuest BioSciences, Inc.FrederickUSA

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