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Diagnosis and Treatment of Metronidazole-Resistant Trichomonas vaginalis Infection

  • Chapter
Antimicrobial Drug Resistance

Part of the book series: Infectious Disease ((ID))

Trichomonas vaginalis is one of four protozoan species of the family Trichomonadidae known to parasitize humans. Members of this family are characterized by their variable morphology, being spheroid or ovoid in form in axenic culture, but assuming an ameboid shape on contact with other cells (4, 101). Trichomonads reproduce by longitudinal binary fi ssion and lack a cystic stage, although large, round “pseudocysts” have been known to form under unfavorable conditions. All Trichomonadidae possess fi ve anterior fl agella, four of which are free moving. The fi fth recumbent fl agellum is anchored along the organism as a part of the undulating membrane. This membrane extends along at least half the length of the organism, and is supported by a noncontractile costa. Motility, described as “bobbing” or “quivering”, is characteristic of this family of organisms (38, 39).

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References

  1. Ackers, J. P. (1990) Immunologic aspects of human trichomoniasis, in Trichomonads parasitic in humans (Honigberg, B. M., ed.), Springer, New York, NY, pp. 36–52.

    Google Scholar 

  2. Alderete, J. F. and Provenzano, D. (1997) The vagina has reducing environment sufficient for activation of Trichomonas vaginalis cysteine proteinases. Genitourin. Med. 73, 291–296.

    PubMed  CAS  Google Scholar 

  3. Alper, M. M., Barwin, B. N., McLean, W. M., McGilveray, I. J., and Sved, S. (1985) Systemic absorption of metronidazole by the vaginal route. Obstet. Gynecol. 65(6), 781–784.

    PubMed  CAS  Google Scholar 

  4. Arroyo, R., Gonzalez-Robles, A., Martinez-Palomo, A., and Alderete, J. F. (1993) Signalling of Trichomonas vaginalis for amoeboid transformation and adhesion synthesis follows cytoadherence. Mol. Microbiol. 7, 299–309.

    PubMed  CAS  Google Scholar 

  5. Beach, D. H., Holz, G. G. Jr., Singh, B. N., and Lindmark, D. G. (1990) Fatty acid and sterol metabolism of Trichomonas vaginalis and Tritrichomonas foetus. Mol. Biochem. Parasitol. 38, 175–190.

    PubMed  CAS  Google Scholar 

  6. Bouma, M., Snowdon, J. D., Fairlamb, A. H., and Ackers, J. P. (1998) Activity of disulfiram ((bis)diethylthiocarbamoyl)disulphide) and didiocarb (diethyldithiocarbamate) against metronidazole sensitive and resistant Trichomonas vaginalis and Tritrichomonas foetus. J. Antimicrob. Chemother. 42, 817–820.

    PubMed  CAS  Google Scholar 

  7. Bramley, M. and Kinghorn, G. (1979) Do oral contraceptives inhibit Trichomonas vaginalis? Sex. Transm. Dis. 6, 261–263.

    PubMed  CAS  Google Scholar 

  8. Cameron, D. E. and Padian, N. S. (1990) Sexual transmission of HIV and the epidemiology of other sexually transmitted diseases. AIDS 4(Suppl 1), S99–S103.

    PubMed  Google Scholar 

  9. Campero, C. M., Hirst, R. G., Ladds, P. W., Vaughn, J. A., Emery, D. L., and Watson, D. L. (1990) Measurement of antibody in serum and genital fluids of bulls with ELISA after vaccination and challenge with Tritrichomonas foetus. Aust. Vet. J. 67(5), 175–178.

    PubMed  CAS  Google Scholar 

  10. Carney, J. A., Unadkat, P., Yule, A., et al. (1988) A rapid agglutination test for the diagnosis of Trichomonas vaginalis infection. J. Clin. Pathol. 41, 806–808.

    PubMed  CAS  Google Scholar 

  11. Caro-Paton, T., Carvajal, A., Diego, I. M., Martin-Arias, L. H., Requejo, A. A., and Pinilla, E. R. (1997) Is metronidazole tera-togenic? A meta-analysis. Br. J. Clin. Pharmacol. 44, 179–182.

    PubMed  CAS  Google Scholar 

  12. Chen, K. C., Amsel, R., Eschenbach, D. A., and Holmes, K. K. (1982) Biochemical diagnosis of vaginitis: determination of diamines in vaginal fluid. J. Infect. Dis. 145, 337–345.

    PubMed  CAS  Google Scholar 

  13. Chen, M. Y., Smith, N. A., Fox, E. F., Bingham, J. S., and Barlow, D. (2001) Acetarsol pessaries in the treatment of metronidazole-resist-ant Trichomonas vaginalis. Int. J. STD AIDS 10, 277–280.

    Google Scholar 

  14. Cleveland, L. R. (1928) Tritrichomonas fecalis nov. sp. of man; its ability to grow and multiply indefinitely in faeces diluted with tap water and in frogs and tadpoles. Am. J. Hyg. 8, 232–255.

    Google Scholar 

  15. Conner, T. H., Stoeckel, M., Evrard, J., and Legator, M. S. (1977) The contribution of metronidazole and two metabolites to the mutagenic activity detected in the urine in treated humans and mice. Cancer Res. 37, 629–633.

    CAS  Google Scholar 

  16. Cosar, C. and Julou, L. (1959) Activity of 1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole (8823 RP) against experimental Trichomonas vaginalis infection. Ann. Inst. Pasteur. 96, 238–241.

    CAS  Google Scholar 

  17. Crowell, A. L., Stephens, C. E., Kumar, A., Boykin, D. W., and Secor, W. E. (2004) Activities of dicationic compounds against Trichomonas vaginalis. Antimicrob. Agents Chemother. 48(9), 3602–3605.

    PubMed  CAS  Google Scholar 

  18. Cunningham, F. E., Kraus, D. M., Brubaker, L., and Fischer, J. H. (1994) Pharmacokinetics of intravaginal metronidazole gel. J. Clin. Pharmacol. 34, 1060–1065.

    PubMed  CAS  Google Scholar 

  19. Czeizel, A. E. and Rockenbauer, M. (1998) A population based case — control teratologic study of oral metronidazole treatment during pregnancy. Br. J. Obstet. Gynecol. 105, 322–327.

    CAS  Google Scholar 

  20. Dobell, C. C. (1939) The common flagellate of the human mouth, Trichomonas tenax (O.F.M.): its discovery and its nomenclature. Parasitology 31, 13–146.

    Google Scholar 

  21. Dubini, F., Riviera, L., Cocuzza, C., and Bellotti, M. G. (1992) Antibacterial, antimycotic, and trichomonicidal activity of a new nitroimidazole (EU 11100). J. Chemother. 4(6), 342–346.

    PubMed  CAS  Google Scholar 

  22. Dunne, R. L., Dunne, L. A., Upcroft, P., O'Donoghue, P. J., and Upcroft, J. A. (2003) Drug resistance in the sexually transmitted parasitic protozoan Trichomonas vaginalis. Cell Res. 13(4), 239–249.

    PubMed  CAS  Google Scholar 

  23. Edwards, D. I. (1980) Mechanisms of selective toxicity of met-ronidazole and other nitroimidazole drugs. Br. J. Vener. Dis. 56, 285–290.

    PubMed  CAS  Google Scholar 

  24. Edwards, D. I. (1993) Nitroimidazole drugs — action and resistance mechanisms. I. Mechanisms of action. J. Antimicrob. Chemother. 31, 9–20.

    PubMed  CAS  Google Scholar 

  25. Ellis, J. E., Yartlett, N., Cole, D., Humphreys, M. J., and Lloyd, D. (1994) Antioxidant defences in the microaerophilic protozoan Trichomonas vaginalis: comparison of metronidazole-resistant and sensitive strains. Microbiology 140, 2489–2494.

    PubMed  CAS  Google Scholar 

  26. Fouts, A. C. and Kraus, S. J. (1980) Trichomonas vaginalis: a re-evaluation of its clinical presentation and laboratory diagnosis. J. Infect. Dis. 141, 137–143.

    PubMed  CAS  Google Scholar 

  27. Gallai, Z. and Sylvestre, L. (1966) The present status of urogential trichomoniasis: a general review of the literature. Appl. Ther. 8, 773–778.

    PubMed  CAS  Google Scholar 

  28. Gerbase, A. C., Rowley, J. T., Heymann, D. H., Berkeley, S. B. F., and Piot, P. (1998) Global prevalence and incidence estimates of selected curable STDs. Sex. Transm. Dis. 74(Suppl 1), S12–S16.

    Google Scholar 

  29. Grossman, J. H. and Galask, R. P. (1990) Persistent vaginitis caused by metronidazole resistant Trichomonas. Obstet. Gynecol. 76, 521–522.

    Google Scholar 

  30. Gupta, P. K. and Frost, J. K. (1990) Cytopathology and his-topathology of the female genital tract in T. vaginalis infection, in Trichomonads parasitic in humans (Honigberg, B. M., ed.), Springer, New York, NY, pp. 274–290.

    Google Scholar 

  31. Heine, P. and MacGregor, J. A. (1993) Trichomonas vaginalis: a re-emerging pathogen. Clin. Obstet. Gynecol. 36, 137–144.

    PubMed  CAS  Google Scholar 

  32. Heyworth, P. G., Gutteridge, W. E., and Ginger, C. D. (1982) Purine metabolism in Trichomonas vaginalis. FEBS Lett. 141, 106–110.

    PubMed  CAS  Google Scholar 

  33. Heyworth, P. G., Gutteridge, W. E., and Ginger, C. D. (1982) Pyrimadine metabolism in Trichomonas vaginalis. FEBS Lett. 176, 55–60.

    Google Scholar 

  34. Hipp, S. S., Kirkwood, M. W., and Hassan, H. A. (1979) Screening for Trichomonas vaginalis infection by use of acridine orange fluorescent microscopy. Sex. Transm. Dis. 6, 235–238.

    PubMed  CAS  Google Scholar 

  35. Hoffmann, B., Kazanawska, W., Kilczewski, W., and Krach, J. (1963) Serologic diagnosis of Trichomonas infection. Med. Dosw. Mikrobiol. 15, 91–99.

    PubMed  CAS  Google Scholar 

  36. Honigberg, B. M. (1978) Trichomonads of importance in human medicine, in Parasitic protozoa, vol. 2 (Krieger, J. P., ed.), Academic, New York, NY, pp. 275.

    Google Scholar 

  37. Honigberg, B. M. (1987) Immunology of trichomonads, with emphasis on Trichomonas vaginalis: a review. Acta Univ. Carol. Biol. 30, 321–336.

    Google Scholar 

  38. Honigberg, B. M. and King, V. M. (1964) Structure of Trichomonas vaginalis Donne. J. Parasitol. 50, 34–364.

    Google Scholar 

  39. Honigberg, B. M. and Brugerolle, G. (1990) Structure, in Trichomonads parasitic in humans (Honigberg, B. M., ed.), Springer, New York, NY, pp 5–35.

    Google Scholar 

  40. Ings, R. M., McFadzean, J. A., and Ormerod, W. E. (1974) The mode of action of metronidazole in Trichomonas vaginalis and other micro-organisms. Biochem. Pharmacol. 23, 1421–1429.

    PubMed  CAS  Google Scholar 

  41. Jaakmees, H. P., Teras, J. K., Roigas, E. M., Nigeson, U. K., and Tompel, H. J. (1966) Complement fixing antibodies in the blood sera of men infested with Trichomonas vaginalis. Wiad. Parazytol. 12, 378–384.

    Google Scholar 

  42. Jirovec, O. and Petru, M. (1968) Trichomonas vaginalis and tricho-moniasis. Adv. Parasitol. 6, 117–188.

    PubMed  CAS  Google Scholar 

  43. Johnson, P. J., Lahti, C. J., and Bradley, P. J. (1993) Biogenesis of the hydrogenosome in the anaerobic protest Trichomonas vagina-lis. J. Parasitol. 79, 644–670.

    Google Scholar 

  44. Kane, P. O., McFadzean, J. A., and Squires, S. (1961) Absorption and excretion of metronidazole. Part II. Studies on primary failures. Br. J. Vener. Dis. 37, 276–277.

    PubMed  CAS  Google Scholar 

  45. Kerscher, L. and Oesterhelt, D. (1982) Pyruvate-ferredoxin oxi-doreductase — new findings on an ancient enzyme. Trends Biochem. Sci. 7, 371–374.

    CAS  Google Scholar 

  46. Kozlowska, D. and Wichrowska, B. (1976) The effect of chlorine and its compounds used for disinfection on Trichomonas vaginalis. Wiad. Parazytol. 22, 433–435.

    PubMed  CAS  Google Scholar 

  47. Kramar, J. and Kucera, K. (1966) Immunofluorescence demonstration of antibodies in urogenital trichomoniasis. J. Hyg. Epidemiol. Microbiol. Immunol. 10, 85–88.

    PubMed  CAS  Google Scholar 

  48. Krieger, J. N. (1984) Prostatitis syndromes: pathophysiology, differential diagnosis, and treatment. Sex. Transm. Dis. 11, 100–112.

    PubMed  CAS  Google Scholar 

  49. Krieger, J. N. (1990) Epidemiology and clinical manifectations of urogenital trichomoniasis in men, in Trichomonads parasitic in humans (Honigberg, B. M., ed.), Springer, New York, NY, pp 235–245.

    Google Scholar 

  50. Krieger, J. N. and Rein, M. F. (1982) Zinc sensitivities of Trichomonas vaginalis: in vitro studies and clinical implications. J. Infect. Dis. 146, 341–345.

    PubMed  CAS  Google Scholar 

  51. Krieger, J. N., Tam, M. R., Stevenes, C. E., Nielsen, I. O., Hale, J., Kiviat, N. B., and Holmes, K. K. (1988) Diagnosis of trichomo-niasis: comparison of conventional wet-mount examination with cytologic studies, cultures, and monoclonal antibody staining of direct specimens. JAMA 259, 1223–1227.

    PubMed  CAS  Google Scholar 

  52. Krieger, J. N., Jenny, C., Verdon, M., et al. (1993) Clinical manifestations of trichomoniasis in men. Ann. Intern. Med. 188, 844–849.

    Google Scholar 

  53. Kulda, J. (1999) Trichomonads, hydrogenosomes, and drug resistance. Int. J. Parasitol. 29, 199–212.

    PubMed  CAS  Google Scholar 

  54. Kulda, J., Tachezy, J., and Czerasovova, A. (1993) In vitro induces anaerobic resistance to metronidazole in Trichomonas vaginalis. J. Eukaryot. Microbiol. 40, 262–269.

    PubMed  CAS  Google Scholar 

  55. Kvasnicka, W. G., Hanks, D., Huang, J. C., Hall, M. R., Sandblom, D., Chu, H. J., Chavez, L., and Acree, W. M. (1992) Clinical evaluation of the efficacy of inoculating cattle with a vaccine containing Tritrichomonas foetus. Am. J. Vet. Res. 53(11), 2023–2027.

    PubMed  CAS  Google Scholar 

  56. Laga, M., Manoka, A. T., Kivuvu, M., Malele, B., Tuliza, M., Nzila, N., Goeman, J. B., Batter, F. V., Alary, M., et al. (1993) Non-ulcerative sexually transmitted diseases as risk factors to HIV-1 transmission in women: results from a cohort study. AIDS 7, 95–102.

    PubMed  CAS  Google Scholar 

  57. Land, K. M., Clemens, D. L., and Johnson, P. J. (2001) Loss of multiple hydrogenosomal proteins associated with organelle metabolism and high-level drug resistance in trichomonads. Exp. Parasitol. 97, 102–110.

    PubMed  CAS  Google Scholar 

  58. Land, K. M., Delgadillo-Correa, M. J., Tachezy, J., Nanacova, S., Hseih, C. L., Sutak, R., and Johnson, P. J. (2004) Targeted gene replacement of a ferredoxin gene in Trichomonas vaginalis does not lead to metronidazole resistance. Mol. Microbiol. 51(1), 115–120.

    PubMed  CAS  Google Scholar 

  59. Lehker, M. W., Arroyo, R., and Aldrete, J. F. (1991) The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan parasite Trichomonas vaginalis. J. Exp. Med. 171, 2165–2170.

    Google Scholar 

  60. Lindmark, D. G. and Muller, M. (1976) Antitrichomonad action, mutagenicity, and reduction of metronidazole and other nitroimi-dazoles. Antimicrob. Agents Chemother. 10, 476–482.

    PubMed  CAS  Google Scholar 

  61. Lindmark, D. G., Muller, M., and Shio, H. (1975) Hydrogenosomes in Trichomonas vaginalis. J. Parasitol. 61, 522–554.

    Google Scholar 

  62. Linstead, D. and Cranshaw, M. A. (1983) The pathway of arginine catabolism in the parasitic flagellate Trichomonas vaginalis. Mol. Biochem. Parasitol. 8, 241–252.

    PubMed  CAS  Google Scholar 

  63. Lloyd, D. and Kristensen, B. (1985) Metronidazole inhibition of hydrogen production in vivo in drug- sensitive and -resistant strains of Trichomonas vaginalis. J. Gen. Microbiol. 131, 849–853.

    PubMed  CAS  Google Scholar 

  64. Lossick, J. G. (1990) Epidemiology of urogenital trichomoniasis. Therapy of urogenital trichomoniasis, in Trichomonads parasitic in humans (Honigberg, B. M., ed.), Springer, New York, NY, pp. 324–341.

    Google Scholar 

  65. Lossick, J. G. (1990) Treatment of sexually transmitted diseases. Rev. Infect. Dis. 12(Suppl 6), S665–S681.

    PubMed  Google Scholar 

  66. Lossick, J. G. and Kent, H. L. (1991) Trichomoniasis: trends in diagnosis and management. Am. J. Obstet. Gynecol. 165(4), 1217–1222.

    PubMed  CAS  Google Scholar 

  67. Lossick, J. G., Muller, M., and Gorrell, T. E. (1986) In vitro drug susceptibility and doses of metronidazole required for cure in cases of refractory vaginal trichomoniasis. J. Infect. Dis. 153, 948–955.

    PubMed  CAS  Google Scholar 

  68. Lushbaugh, W. B., Cleary, J. D., and Finley, R. W. (1995) Cytotoxicity of hamycin for Trichomonas vaginalis, HeLa and BHK-21. J. Antimicrob. Chemother. 36, 795–802.

    PubMed  CAS  Google Scholar 

  69. Mack, S. R. and Muller, M. (1980) End products of carbohydrate metabolism in Trichomonas vaginalis. Comp. Biochem. Physiol. 67, 213–216.

    Google Scholar 

  70. Malagoli, M., Ross, T., Baggio, A., Zandomeneghi, G., Zanca, A., Casolari, C., and Castelli, M. (2002) In vitro study of chemotherapeutic activity of sulphimidazole on some sensitive and metronidazole resistant Trichomonas vaginalis strains. Pharmacol. Res. 46, 469–472.

    PubMed  CAS  Google Scholar 

  71. Malla, N., Gupta, I., Sokhey, C., Sehgal, R., Ganguly, N. K., and Mahajan, R. J. (1988) In vitro evaluation of metronidazole and tinidazole on strains of Trichomonas vaginalis. Indian J. Med. Microbiol. 6, 297–301.

    Google Scholar 

  72. Martin, D. H. and Bowie, W. R. (1999) Urethritis in males, in Sexually transmitted disease, 3rd ed. (Holmes, K. K., Mardh, P. A., Sparling, P. F., and Weisner, P. J., eds.) McGraw-Hill, New York, NY, pp. 833–845.

    Google Scholar 

  73. Mason, P. R., Super, H., and Fripp, P. J. (1976) Comparison of four techniques for the routine diagnosis of Trichomonas vaginalis infection. J. Clin. Pathol. 29, 154–157.

    PubMed  CAS  Google Scholar 

  74. McGrory, T. and Garber, G. E. (1992) Mouse intravaginal infection with Trichomonas vaginalis and role of Lactobacillus acidophilus in sustaining infection. Infect. Immun. 60(6), 2375–2379.

    PubMed  CAS  Google Scholar 

  75. McMillan, A. (1990) Laboratory diagnostic methods and cryop-reservation of trichomonads, in Trichomonads parasitic in humans (Honigberg, B. M., ed.), Springer, New York, N Y, pp 297–310.

    Google Scholar 

  76. Meysick, K. and Garber, G. E. (1995) Trichomonas vaginalis. Curr. Opin. Infect. Dis. 8, 22–25.

    Google Scholar 

  77. Muller, M. (1986) Reductive activation of nitroimidazoles in anaerobic microorganisms. Biochem. Pharmacol. 35, 37–41.

    PubMed  CAS  Google Scholar 

  78. Muresu, R., Rubino, S., Rizzu, P., Baldini, A., Colombo, M., and Capuccinelli, P. (1994) A new method for identification of Trichomonas vaginalis by fluorescent DNA in-situ hybridization. J. Clin. Microbiol. 32, 1018–1022.

    PubMed  CAS  Google Scholar 

  79. Nagesha, C. W., Ananthakrishna, N. C., and Sulochana, P. (1970) Clinical and laboratory studies on vaginal trichomoniasis. Am. J. Obstet. Gynecol. 106, 933–935.

    PubMed  CAS  Google Scholar 

  80. Narcisi, E. M. and Secor, W. E. (1996) In vitro effect of tinidazole and furazolidone on metronidazole-resistant Trichomonas vagina-lis. Antimicrob. Agents Chemother. 40(5), 1121–1125.

    PubMed  CAS  Google Scholar 

  81. Nielson, R. (1969) Trichomonas vaginalis I: survival in solid Stuart's medium. Br. J. Vener. Dis. 45, 328–331.

    Google Scholar 

  82. O'Connor, B. H. and Adler, M. W. (1979) Current approaches to the diagnosis and reporting of trichomoniasis and candidosis. Br. J. Vener. Dis. 55, 52–57.

    PubMed  Google Scholar 

  83. Perez-Reyes, E., Kalyanaraman, B., and Mason, R. P. (1980) The reductive metabolism of metronidazole and ronidazole by aerobic liver microsomes. Mol. Pharmacol. 17, 239–244.

    PubMed  CAS  Google Scholar 

  84. Petrin, D., Delgaty, K., Bhatt, R., and Garber, G. E. (1998) Clinical and microbiological aspects of Trichomonas vaginalis. Clin. Micrbiol. Rev. 11(2), 300–317.

    CAS  Google Scholar 

  85. Poppe, W. A. J. (2001) Nitroimidazole resistant vaginal trichomo-niasis treated with paramomycin. Eur. J. Obstet. Gynecol. Reprod. Biol. 96, 119–120.

    PubMed  CAS  Google Scholar 

  86. Rasoloson, D., Tomkova, E., Cammack, R., Kulda, J., and Tachezy, J. (2001) Metronidazole resistant strains of Trichomonas vaginalis display increased sensitivity to oxygen. Parasitology 123(1), 45–56.

    PubMed  CAS  Google Scholar 

  87. Rayner, C. F. A. (1968) Comparison of culture media for the growth of Trichomonas vaginalis. Br. J. Vener. Dis. 44, 63–66.

    PubMed  CAS  Google Scholar 

  88. Rein, M. F. (1977) Trichomoniasis in VD clinic women. Paper presented at the Annual Meeting of the American Public Health Association, Washington DC, November 1.

    Google Scholar 

  89. Rein, M. F. and Chapel, T. A. (1975) Trichomoniasis, candidiasis, and the minor venereal diseases. Clin. Obstet. Gynecol. 18, 73–88.

    PubMed  CAS  Google Scholar 

  90. Rein, M. F. and Holmes, K. K. (1983) “Nonspecific vaginitis” vulvovaginal candidiasis and trichomoniasis: clinical features, diagnosis, and management, in Current clinical topics in infectious diseases, vol. 4 (Remington, J. S. and Swartz, M. N., eds.) McGraw-Hill, New York, NY, pp. 281–315.

    Google Scholar 

  91. Rein, M. F. and Muller, M. (1999) Trichomonas vaginalis and tri-chomiasis, in Sexually transmitted disease, 3rd ed. (Holmes, K. K., Mardh, P. A., Sparling, P. F., and Weisner, P. J., eds.) McGraw-Hill, New York, NY, pp. 481–492.

    Google Scholar 

  92. Riley, D. E., Roberts, M. C., Takayama, T., and Krieger, J. N. (1992) Development of a polymerase chain reaction-based diagnosis of Trichomonas vaginalis. J. Clin. Microbiol. 30, 465–472.

    PubMed  CAS  Google Scholar 

  93. Rodgerson, E. B. (1972) Vulvovaginal papillomas and Trichomonas vaginalis. Obstet. Gynecol. 40, 327–333.

    PubMed  CAS  Google Scholar 

  94. Rosa, F. W., Baum, C., and Shaw, M. (1987) Pregnancy outcomes after first-trimester vaginitis drug therapy. Obstet. Gynecol. 69(5), 751–755.

    PubMed  CAS  Google Scholar 

  95. Sorvillo, F. and Kerndt, P. (1998) Trichomonas vaginalis and amplification of HIV-1 transmission. Lancet 351, 213–214.

    PubMed  CAS  Google Scholar 

  96. Street, D. A., Taylor-Robinson, D., Ackers, J. P., Hanna, N. F., and McMillan, A. (1982) Evaluation of an enzyme-linked immuno-sorbent assay for the detection of antibody to Trichomonas vagina-lis in sera and vaginal secretions. B r. J. Vener. Dis. 58, 330–333.

    CAS  Google Scholar 

  97. Teras, J. K., Jaakmees, H. P., Nigeson, U. K., Roigas, E. M., and Tompel, H. J. (1966) Dependence of serologic reactions on the sero-types of Trichomonas vaginalis. Wiad. Parazytol. 12, 364–369.

    Google Scholar 

  98. Tsukahara, T. (1961) Respiratory metabolism of Trichomonas vaginalis. Jpn. J. Microbiol. 5, 157–169.

    PubMed  CAS  Google Scholar 

  99. Vidakovic, M., Crossnoe, C. R., Neidre, C., Kim, K., Krause, K. L., and Germanas, J. P. (2003) Reactivity of reduced [2Fe-2S] ferredoxins parallel host susceptibility to nitroimidazoles. Antimicrob. Agents Chemother. 47(1), 302–308.

    PubMed  CAS  Google Scholar 

  100. Vontver, L. A. and Eschenbach, D. A. (1981) The role of Gardnerella vaginalis in nonpesific vaginitis. Clin. Obstet. Gynecol. 24, 439–460.

    PubMed  CAS  Google Scholar 

  101. Warton, A. and Honigberg, B. M. (1979) Structure of trichomonads as revealed by scanning electron microscopy. J. Protozool. 26, 5–62.

    Google Scholar 

  102. Watson, P. G. and Pattman, R. S. (1996) Arsenical pessaries in the successful elimination of metronidazole-resistant Trichomonas vaginalis. Int. J. STD AIDS 10, 277–280.

    Google Scholar 

  103. Weinberger, M. W. and Harger, J. H. (1993) Accuracy of the Papanicolaou smear in the diagnosis of asymptomatic infection with Trichomonas vaginalis. Obstet. Gynecol. 82, 425–429.

    PubMed  CAS  Google Scholar 

  104. Wenrich, D. H. and Saxe, L. H. (1950) Trichomonas microti, n. sp. (Protozoa, Mastigophora). J. Parasitol. 36, 261–269.

    PubMed  CAS  Google Scholar 

  105. Werness, B. A. (1989) Cytopathology of sexually transmitted diseases. Clin. Lab. Med. 9, 559.

    PubMed  CAS  Google Scholar 

  106. Weston, T. E. and Nicol, C. S. (1963) Natural history of trichomo-nal infection in males. Br. J. Vener. Dis. 39, 251–257.

    PubMed  CAS  Google Scholar 

  107. Whittington, M. J. (1951) The survival of Trichononas vaginalis at temperatures below 37°C. J. Hyg. 49, 400–409.

    CAS  Google Scholar 

  108. Whittington, M. J. (1957) Epidemiology of Trichomonas vagi-nalis infections in the light of improved diagnostic methods. Br. J. Vener. Dis. 33, 80–91.

    PubMed  CAS  Google Scholar 

  109. Wolner-Hanssen, P., Krieger, J. N., Stevens, C. E., Kiviat, N. B., Koutsky, L., Critchlow, T., DeRouen, T., Hillier, S., and Holmes, K. K. (1989) Clinical manifestations of vaginal trichomoniasis. JAMA 261(4), 571–576.

    PubMed  CAS  Google Scholar 

  110. Yarlett, N., Rowlands, C. C., Yarlett, N. C., Evans, J. C., and Lloyd, D. (1987) Reduction of niridazole by metronidazole resistant and susceptible strains of Trichomonas vaginalis. Parasitology 94, 93–99.

    PubMed  CAS  Google Scholar 

  111. Yule, A., Gellan, M. C. A., Driel, J. D., et al. (1987) Detection of Trichomonas vaginalis antigen in women by enzyme immunoassay. J. Clin. Pathol. 40, 566–568.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Sarah L. Cudmore

  2. Division of Infectious Diseases, Ottawa Hospital, General Campus, Ottawa, ON, Canada

    Gary E. Garber

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  1. Sarah L. Cudmore
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  2. Gary E. Garber
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  1. Executive Vice President & Chief Medical Officer, Idenix Pharmaceuticals, Cambridge, MA, USA

    Douglas L. Mayers MD

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Cudmore, S.L., Garber, G.E. (2009). Diagnosis and Treatment of Metronidazole-Resistant Trichomonas vaginalis Infection. In: Mayers, D.L. (eds) Antimicrobial Drug Resistance. Infectious Disease. Humana Press. https://doi.org/10.1007/978-1-60327-595-8_28

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  • DOI: https://doi.org/10.1007/978-1-60327-595-8_28

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