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Biomedical Applications of Biophysics pp 137–154Cite as

Introduction to Modern Techniques in Mass Spectrometry

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Part of the book series: Handbook of Modern Biophysics ((HBBT,volume 3))

Abstract

Mass spectrometry has emerged as an invaluable technique with a wide array of applications ranging from clinical to biodefense. With the development of different ionization techniques and mass analyzers, even challenging samples can be analyzed, thereby making mass spectrometry an important analytical tool in the field of biophysics. Mass spectrometry is the only technique that offers the combination of high sensitivity (attomole) with structural information. While other analytical techniques may provide higher sensitivity, these techniques do not provide structural information. Conversely, other techniques may provide more complete structures but have significantly less sensitivity. The different ionization techniques allow for the examination of analytes ranging from small metabolites to large macromolecular assemblies. In this chapter the major components are described rather than the possible applications, which would require volumes. With the major concepts in hand, the student is encouraged to read specific reviews regarding the kinds of applications of interest to the researcher.

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Authors and Affiliations

  1. Department of Chemistry, University of California Davis, One Shields Avenue, Davis, California, 95616, USA

    Caroline S. Chu & Carlito B. Lebrilla

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  1. Caroline S. Chu
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  2. Carlito B. Lebrilla
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Correspondence to Carlito B. Lebrilla .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Medicine, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA

    Thomas Jue Ph.D.

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Chu, C.S., Lebrilla, C.B. (2010). Introduction to Modern Techniques in Mass Spectrometry. In: Jue, T. (eds) Biomedical Applications of Biophysics. Handbook of Modern Biophysics, vol 3. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-233-9_6

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