Skip to main content

Introduction to Electron Paramagnetic Resonance Spectroscopy

  • Chapter
  • First Online:
Biomedical Applications of Biophysics

Part of the book series: Handbook of Modern Biophysics ((HBBT,volume 3))

Abstract

The acronym EPR describes a spectroscopic technique known as electron paramagnetic resonance.In several, especially older, textbooks and research papers the alternative names ESR(electron spin resonance) or EMR (electron magnetic resonance) can be sometimes encountered.The development of EPR spectroscopy was slowed during the few decades following its inventiondue to the unavailability of microwave components for higher frequencies. Today, however, EPR technologies are enjoying very rapid development in all areas of application. Such regained interest is related to the fact that EPR not only proved to be an extremely powerful spectroscopic technique but to an increasing amount of related techniques that were born around EPR methodology. Recent developments in the area of instrumentation followed by the commercialization of high-end EPR spectrometers have attracted many new adepts who benefit from exploring this technique in their specific fields of research.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 179.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 229.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Further Study

  1. Wertz JE, Bolton JR. 2007. Electron paramagnetic resonance: elementary theory and practical applications. New York: Chapman and Hall.

    Google Scholar 

  2. Atherton NM. 1993. Principles of electron spin resonance. Ellis Horwood Series in Physical Chemistry. Chichester: Ellis Horwood and PTR Prentice Hall.

    Google Scholar 

  3. Bersohn M, Baird JC. 1966. An introduction to electron paramagnetic resonance. New York: W.A. Benjamin.

    Google Scholar 

  4. Gordy W. 1980. Theory and applications of electron spin resonance. New York: John Wiley & Sons.

    Google Scholar 

  5. Poole CP. 1983. Electron spin resonance. Mineola, NY; Dover.

    Google Scholar 

  6. Schweiger A, Jeschke G. 2001. Principles of pulse electron paramagnetic resonance. Oxford: Oxford UP.

    Google Scholar 

  7. Eaton GR, Eaton SS, Salikhov KM. 1998. Foundations of modern EPR. Singapore: World Scientific.

    Book  Google Scholar 

  8. . An excellent compilation on the common EPR techniques at the introductory level is provided on the Bruker webpage: <http://www.bruker-biospin.com/whatisepr.html>.

  9. Abragam A, Bliney B. 1986. Electron paramagnetic resonance of transition ions. Oxford: Clarendon.

    Google Scholar 

  10. Mabbs FE, Collison D. 1992. Electron paramagnetic resonance of transition metal compounds. Studies in Inorganic Chemistry. Kidlington, Oxfordshire: Elsevier Science.

    Google Scholar 

  11. Pilbrow JR. 1990. Transition-ion electron paramagnetic resonance. Oxford: Clarendon.

    Google Scholar 

References

  1. Hristova D. 2005. PhD Thesis, University of Basel, Switzerland.

    Google Scholar 

  2. Inanami O, Hashida S, Iizuka D, Horiuchi M, Hiraoka W, Shimoyama Y, Nakamura H, Inagaki F, Kuwabara M. 2005. Conformational change in full-length mouse prion: a site-directed spin-labeling study. Biochem Biophys Res Commun 335:785-792.

    Article  CAS  PubMed  Google Scholar 

  3. Smith SR, Pala I, Benore-Parsons M. 2006. Riboflavin binding protein contains a type II copper binding site. J Inorg Biochem 100:1730-1733.

    Article  CAS  PubMed  Google Scholar 

  4. Chevallet M, Dupuis A, Issartel J-P, Lunardi J, van Belzen R, Albracht SPJ. 2003. Two EPR-detectable [4Fe- 4S] clusters, N2a and N2b, are bound to the NuoI (TYKY) subunit of NADH:ubiquinone oxidoreductase (Complex I) from Rhodobacter capsulatus. Biochim Biophys Acta Bioenerg 1557:51-66.

    Article  CAS  Google Scholar 

  5. Lefevre-Groboillot D, Frapart Y, Desbois A, Zimmermann JL, Boucher JL, Gorren AC, Mayer B, Stuehr DJ, Mansuy D. 2003. Two modes of binding of N-hydroxyguanidines to NO synthases: first evidence for the formation of iron-N-hydroxyguanidine complexes and key role of tetrahydrobiopterin in determining the binding mode. Biochemistry 42:3858-3867.

    Article  CAS  PubMed  Google Scholar 

  6. Hollenberg PF, Hager LP, Blumberg WE, Peisach J. 1980. An electron paramagnetic resonance study of the high and low spin forms of chloroperoxidase. J Biol Chem 255:4801-4807.

    CAS  PubMed  Google Scholar 

  7. Chang CH, Svedruzic D, Ozarowski A, Walker L, Yeagle G, Britt RD, Angerhofer A, Richards NG. 2004. EPR spectroscopic characterization of the manganese center and a free radical in the oxalate decarboxylase reaction: identification of a tyrosyl radical during turnover. J Biol Chem 279:52840-52849.

    Article  CAS  PubMed  Google Scholar 

  8. Britt RD, Campbell KA, Peloquin JM, Gilchrist ML, Aznar CP, Dicus MM, Robblee J, Messinger J. 2004. Recent pulsed EPR studies of the Photosystem II oxygen-evolving complex: implications as to water oxidation mechanisms. Biochim Biophys Acta Bioenerg 1655:158-171.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Marcin Brynda .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Springer Science+Business Media, LLC

About this chapter

Cite this chapter

Brynda, M. (2010). Introduction to Electron Paramagnetic Resonance Spectroscopy. In: Jue, T. (eds) Biomedical Applications of Biophysics. Handbook of Modern Biophysics, vol 3. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-233-9_3

Download citation

Publish with us

Policies and ethics