Intracerebral hemorrhage (ICH) has been modeled in the laboratory with the intracerebral injection of autologous blood, injection of emboli, or proteolysis of the basal lamina surrounding cerebral capillaries by bacterial collagenase (BC). Selection of the model depends on the concept of the pathophysiology behind the bleeding. Modeling a rapidly progressive bleed without excessive tissue destruction can be done by the injection of autologous blood. Injection of small blood clots into the carotid artery of the rabbit mimics an embolic stroke with hemorrhagic transformation. BC produces a highly reproducible model of intracerebral bleeding that evolves over several hours, produces inflammation around the lesion, and slowly resolves, simulating the process that has been observed to occur in human studies of serially imaged patients with ICH. BC initiates an injury that is propagated by the production of endogenous proteolytic enzymes of the matrix metalloproteinase (MMP) gene family. The inflammatory response, which is seen in both the BC model and the autologous blood model, is an important part of the pathological changes produced in the brain by the disruption of the blood vessels and brain edema. MMP inhibitors reduce the damage to the brain by the hemorrhagic mass lesion. A number of other therapeutic agents reduce injury in BC-induced ICH.
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Grossetete, M., Rosenberg, G.A. (2009). Bacterial Collagenase Injection Intracerebral Hemorrhage Rat Model. In: Chen, J., Xu, Z.C., Xu, XM., Zhang, J.H. (eds) Animal Models of Acute Neurological Injuries. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-185-1_29
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DOI: https://doi.org/10.1007/978-1-60327-185-1_29
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