The filament perforation subarachnoid hemorrhage (SAH) mouse model mimics a clinical situation with aneurysmal SAH in humans. In this model, subarachnoid clot induces vasospasm, which has a peak at 3 days after SAH. Moreover, a sudden increase in intracranial pressure immediately after the arterial perforation induces cortical neuronal damage similar to aneurysmal SAH in humans. Therefore, this model is suitable for researches on the various pathophysiologies of SAH. Although uncontrollable volume of SAH basically causes high mortality rate in this model, our techniques perforating the anterior cerebral artery can lead to less mortality and relatively reproducible state after SAH. The complications and limitations in this model are also discussed.
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Kamii H, Kato I, Kinouchi H, Chan PH, Epstein CJ, Akabane A, Okamoto H, Yoshimoto T. Amelioration of vasospasm after subarachnoid hemorrhage in trans-genic mice overexpressing CuZn-superoxide dismutase. Stroke 1999;30:867–872.
Saito A, Kamii H, Kato I, Takasawa S, Kondo T, Chan PH, Okamoto H, Yoshimoto T. Transgenic CuZn-superoxide dismutase inhibits NO synthase induction in experimental subarachnoid hemorrhage. Stroke 2001;32:1652–1657.
Parra A, McGirt MJ, Sheng H, Laskowitz DT, Pearlstein RD, Warner DS. Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: methodological analysis. Neurol Res 2002;24:510–516.
McGirt MJ, Parra A, Sheng H, Higuchi Y, Oury TD, Laskowitz DT, Pearlstein RD, Warner DS. Attenuation of cerebral vasospasm after subarachnoid hemorrhage in mice overexpressing extracellular superoxide dismutase. Stroke 2002;33:2317– 2323.
Mino M, Kamii H, Fujimura M, Kondo T, Takasawa S, Okamoto H, Yoshimoto T. Temporal changes of neurogenesis in the mouse hippocampus after experimental subarachnoid hemorrhage. Neurol Res 2003;25:839–845.
Bederson JB, Germano IM, Guarino L. Cortical blood flow and cerebral perfusion pressure in a new noncraniotomy model of subarachnoid hemorrhage in the rat. Stroke 1995;26:1086–1092.
Veelken JA, Laing RJ, Jakubowski J. The Sheffield model of subarachnoid hemorrhage in rats. Stroke 1995;26:1279–1284.
Prunell GF, Mathiesen T, Diemer NH, Svendgaard NA. Experimental subarachnoid hemorrhage: subarachnoid blood volume, mortality rate, neuronal death, cerebral blood flow, and perfusion pressure in three different rat models. Neurosurgery 2003;52:165–176.
Lin CL, Calisaneller T, Ukita N, Dumont AS, Kassell NF, Lee KS. A murine model of subarachnoid hemorrhage-induced cerebral vasospasm. J Neurosci Methods 2003;123:89–97.
Lin CL, Dumont AS, Calisaneller T, Kwan AL, Hwong SL, Lee KS. Monoclonal antibody against E selectin attenuates subarachnoid hemorrhage-induced cerebral vasospasm. Surg Neurol 2005;64:201–206.
Cahill J, Calvert JW, Zhang JH. Mechanisms of early brain injury after subarach-noid hemorrhage. J Cereb Blood Flow Metab 2006;26:1341–1353.
Kamii H, Kinouchi H, Sharp FR, Chan PH. A model of transient focal cerebral ischemia in the mouse. In: Ohnishi ST, Ohnishi T, eds. Central Nervous System Trauma: Research Techniques. Boca Raton, Fla: CRC Press, 1995:139–146.
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Kamii, H., Tominaga, T. (2009). Filament Perforation Subarachnoid Hemorrhage: Mouse Model. In: Chen, J., Xu, Z.C., Xu, XM., Zhang, J.H. (eds) Animal Models of Acute Neurological Injuries. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-185-1_23
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DOI: https://doi.org/10.1007/978-1-60327-185-1_23
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